IMPACTS OF IMMUNOSUPPRESSION ON THE GRAVITY OF COVID-19 IN INDIVIDUALS CO-INFECTED WITH THE HUMAN IMMUNODEFICIENCY VIRUS (HIV)
Objective: To identify the levels of severity of COVID-19 in individuals co-infected with the Human Immunodeficiency Virus (HIV). Methods: Expanded summary of the literature review type. The searches were carried out in the main databases of the BVS and CAPES. The above authors declare that they do not have conflict of interest in this study. Results: Most individuals were being treated with antiretrovirals (83-100%) and, therefore, most had a TCD4 lymphocyte count above 200 cells/µL and a viral load ranging between 67-100%. Comorbidities ranged from 33-70% and deaths from 0-14%. The severity picture was mostly mild, at a minimum proportion of 63%, not significantly differing from the population with COVID-19 in general, which on average remains in a mild symptomatic picture, around 80%. Therefore, the percentage of deaths and more severe levels of the disease were lower than expected due to the immune deficiency of HIV positive people. Conclusion: It is rash to make generalizations about the topic or to safely close the hypothesis that the immunosuppression of individuals with HIV, co-infected with SARS-CoV-2 has an increased risk to acquire the most severe form of COVID-19. This is because the studies found in the literature did not have a sample of participants significant enough.
PARKINSONIA ACULEATA AS A POTENTIAL PHYTOTHERAPIC FACTOR IN THE INFLAMMATORY AND DYSLIPIDEMIC CONTROL OF WISTAR RATS
Objective: The study evaluated the hypolipidemic and anti-inflammatory potential of the hydroalcoholic extract of P. Aculeata (HEPa/EtOAC) in Wistar rats fed with Westernized diet. Methods: The experiment was approved by the Ethics Committee (Federal University of Pernambuco-23076.027165 / 2010-21). The hydroalcoholic extract (HEPa/EtOAc) was obtained from the aerial parts of P. Aculeata and portioned by acetate/ethanol in different concentrations. 36 male Wistar rats were initially divided into two groups: Control (C, n = 6), fed a commercial diet (26g / 100g of protein; 63g / 100g of carbohydrates; 11g / 100g of lipids) and Westernized (W; n = 30) – fed an experimental diet (15g / 100g of protein; 51g / 100g of carbohydrates; 34 g/100g of lipids) receving water ad libitum. After 4 months of diet, W group was arbitrarily redistributed into six subgroups according to water supplementation, or different extracts concentrations: (n = 6 animals/group): W- distilled water (1mL/kg; po); WG – genfibrozil (140 mg/kg; p.o.); W35- 35 mg/kg; W70 – 70 mg /kg; p.o. and W140- 140 mg/kg; p.o for 30 days. The lipid profile and pro-inflammatory cytokines were analyzed. Results: The westernized diet caused dyslipidemia and the treatment with HEPa-EtOAc (140mg / kg), promoted a significant reduction in the concentration of pro-inflammatory cytokines, in total cholesterol, in the LDLc and serum triglycerides. Conclusion: The reduction of dyslipidemia and pro-inflammatory cytokines from the of P. Aculeata extract corroborate the ethnopharmacological potential of the plant, already used by the population for hypoglycemic purposes.
Infectious diseases are the most common causes of morbidity and mortality in developing countries. Nowadays, medicinal plants play a major role in treatment of infectious diseases and they are easily available and more affordable as compared to synthetic compounds. The emerging trends of multidrug resistance among several groups of microorganisms against different classes of antibiotics led different researchers to develop efficient drugs from plant sources to counter multidrug resistant strains. This study investigated two different concentration of methanol extracts of stem bark of Prosopis cineraria to determine their efficacy against multidrug resistant microbes. Powdered barks of the tree were treated with methanol using hot extraction method. Crude methanol extracts of the bark of P. cineraria was investigated for their antibacterial activity against a wide range of bacteria (both gram-positive and gram-negative) by disc diffusion method. Ciprofloxacin was used as standard. Multidrug resistant (MDR) strains of Bacillus subtilis (ATCC 6633), E. coli (ATCC 8739), Salmonella enterica (ATCC 14028), Staphylococcus aureus (ATCC 6538), Pseudomonas aeruginosa (ATCC 27853) were used in the study. The methanolic bark extracts of P. cineraria showed a remarkable inhibition of the microorganisms. The potency shown by these extracts recommends their use against multidrug resistant microorganisms. The present study suggests that the methanol extract of the stem bark of P. cineraria exhibited a potential antibacterial activity against the tested microorganisms and could be a potential source of new antimicrobial agents.
PREVALENCE AND ANTIBIOTIC RESISTANCE OF CAMPYLOBACTER COLI AND CAMPYLOBACTER JEJUNI IN GREEK SWINE FARMS
Campylobacter species are one of four key global causes of human diarrheal diseases, according to W.H.O. It is considered to be the most common bacterial cause of human gastroenteritis in the world. The objective of this study was to estimate the prevalence of Campylobacter coli (C. coli) and Campylobacter jejuni (C. jejuni) in Greek commercial swine farms, and describe the antimicrobial resistance of the isolated strains. A total of 1,000 rectal swabs (50 per farm) were collected from twenty swine farms in Greece. Ten rectal samples had been randomly collected from each of five age-groups (suckling piglets, nursery pigs, grower pigs, finisher pigs, sows). Isolation of Campylobacter spp. was performed using the ISO 10272-1:2017. A PCR method, based on the amplification of mapAC.jejuni and ceuEC.coli specific genes, was used for identification of the isolated strains. All isolates were tested for their susceptibility against gentamycin, erythromycin, ciprofloxacin, tetracycline and meropenem; EUCAST guidelines were used for the interpretation. The results showed that 16 out of the 20 farms (80%) and 491 (49%) of the samples were positive for Campylobacter spp. Prevalence of C.coli was 38% (95% CI 35.1-41.1) and of C.jejuni 10.9% (95% CI 9,1-13.0). Sows were 1.4 times more likely to be colonized by Campylobacter spp than sucking piglets (p
CURRENT PRACTICES IN GREEK BROILER FARMS AS RELATED TO THE TECHNICAL STATUS OF THE ESTABLISHMENTS AND EQUIPMENT
The aim of this study was to describe breeding and managerial practices performed in two main categories of broiler farms as related to their technical status i.e. years of functioning, facilities, equipment, etc. One hundred thirty two farms, randomly selected, sited in regions which represent more than 70% of the Greek broiler production were examined. A structured questionnaire was used to collect data by personal interviews with the farm managers including biosecurity measures, harvesting system, feeding and watering method, staff, microclimate in broiler house, antimicrobial therapy and information about the establishment and equipment of the farm as well as the genotype, age and population of the flock. First results indicate that biosecurity measures in farms in good technical status perceive more importance (presence of anteroom and foot bath, P≤0.05) than farms in bad status. Moreover, 93.3% of the farms in bad technical status use straw as bedding material in contrast with the 28.2 % of the farms in good status where rice hulls is used (P≤0.05). Both categories of farms rear Ross genotype while Cobb genotype is only reared in farms in good technical status (P≤0.05). Finally, water equipment differs significantly (P≤0.05) between the two farm categories. Nipple drinkers without a tray is used by 40.4% of the farms in good technical status while 58.3% the farms in low level of hygiene have water equipment with nipple drinkers with tray. This study presents preliminary results of a PhD thesis in progress aiming to investigate the prevalence of resistant strains of Escherichia Coli producing ß-lactamases in poultry farms.
Plants play a vital role in many health care systems, be it rural or an urban community. Plants became familiar as medicine due to the ancient ideologies and believes. Several plants parts of plants served as medicines to so many ailments including gastrointestinal ailments, due to the fact that their active ingredients are powerful against the microbes. Most of the microbes identified were gram negative bacteria as well as some gram positive. Some of the principal antibacterial components of plants were recommended being polar compounds. While most of the acknowledged components with antimicrobial activity extracted from plants were aromatic or saturated organic compounds which were more soluble in polar solvents such as water and methanol. As such plants materials in one way or the other are very active when dealing with microbes due to their active ingredients.
During buffaloe OSD spread in a village affiliating to Assiut Governorate-Egypt, 44 buffaloe cows hosted and owned sporadically were subjected to the study. From 43 buffaloe cows (had closed lesions either edematous or nodular) and a buffaloe cow (had open ulcerative lesion), Corynebacterium pseudotuberculosis equi (C. ps. equi) as 72% and Corynebacterium pseudotuberculosis ovis (C. ps. ovis) as 28% were isolated and identified. Blood sucking insects hosted on the infected buffaloe cows (22) louse fly (Hippobosca equine) and 20 Haematopinus eurysternus lice were included during the study where both C. ps. equi and C. ps. ovis were isolated from Hippobosca equina ( H equina) but failed to isolate any biovar of C. ps. from Haematopinus eurysternus lice (H. eur.). Moreover, C. ps. equi was isolated from two H equina pupae – lab deposited – as well as a H equina second generation fly concluding that there is endosymbiosis nature of C. ps. limited only to H. equina fly which can transmit C.ps. vertically.
Background: Rheumatic fever (RF) is a systemic, autoimmune and inflammatory, non-suppurative disease in genetically predisposed individuals with cardiac repercussions between 25-45 years old. It is estimated that 500,000 cases are 21,000 cases diagnosed in Latin America, accounting for 70% of cases of valvular disease in Brazil, predominantly in the northeast and southeast regions of the country. Commonly associated with underdeveloped regions and urban agglomerations, the predominance of this condition will be due to sociodemographic conditions, due to this, variables such as: level of schooling, sanitary conditions, primary health care and nutritional status of the susceptible are relevant to justify the predominance in these areas. Objectives: To outline the epidemiological profile of patients with mitral valvular disease with rheumatic etiology. Methodology: This is a descriptive-exploratory integrative review. The searches were carried out through electronic database PUBMED / MEDLINE and the Virtual Health Library (VHL) from August to September 2018. Of the 62 articles found, only 14 articles were selected because they were able to answer the guiding question: what sociodemographic characteristics are determinant for Streptococcus pyogenes infection? Results: The prevalence of pyogenes streptococcal infection in underdeveloped countries is due to poor sanitary conditions and poor access to primary health care that poorer areas face in the face of social inequality, which is indicated by the level of illiteracy, unemployment and lack of which contribute to the susceptibility to group A streptococcal infection. Conclusion: Social inequality is the determining factor for the prevalence of rheumatic fever in underdeveloped countries, further studies are required that encompass all the variables responsible in order to reduce infection with Streptococcus pyogens in childhood.
The effect of Transfer Factor as Immunotherapy comparing with the effect of BCG in Mice challenged with Mycobacterium tuberculosis
Background: Transfer Factor (TF) is an immune modulator which stimulates the cellular arm of the immune system (killer lymphocytes), activates immune cytokine synthesis and regulates immune function (Lawrence, 1955).TF is very effective in those diseases in which CMI plays a relevant role in protection and control of the disease, such as intracellular bacterial diseases (tuberculosis). ( Estrada Parra, et al1955). TF are low molecular weight products from immune cells that are able to transmit the ability to express delayed-type hypersensitivity (DTH) and cell mediated immunity (CMI) from sensitized donors to non immune recipients (Kirkpatrick, 2000). Objectives: The aim of this experimental study is to determine the protective efficacy of transfer factor (TF) as immunotherapy for mice in comparison to BCG. Materials and methods: A total number of 102 mice were examined for their immunopotency and protective efficacy of Transfer factor (TF) comparing to the protective efficacy of BCG single and second repeated dose against challenge dose of M. tuberculosis (107 CFU) . A number of 20 mice were immunize with the attenuated strain of M. bovis, Bacillus Calmette-Guérin (BCG). After 21 days of BCG spleens of 10 tuberculous mice were removed aseptically for the preparation of TF. To evaluate the effect of TF 3 groups of inbred BALB/c male mice were injected with TF and challenged with virulent M. tuberculosis, followed by another 3 groups of inbred BALB/c male mice which were immunized with BCG single and second repeated dose. All mice with BCG and TF were tested for tuberculin skin test (TST) so as to determine susceptibility and resistance against tuberculosis, susceptible groups of mice were challenged with virulent M. tuberculosis. Followed by study of humoral response by immunization of a group of mice with immune serum and challenged with M. tuberculosis H37Rv strain. Followed by an experiment of…
All around the world about1.7 millions of females are resulted with breast cancer and arround 522,000 deaths. Which is more common in developed countries than the developing ones. Breast cancer mostly occurs in cells from the lining of milk ducts and the lobules that supply the ducts with milk. Symptoms of Breast cancer includes a lump in the breast, dimpling of skin, change in shape of breast, fluids coming from nipple. Risk factor for development of breast cancer includes age, sex, obesity, lack of physical exercise, alcohol consumption, hormone replacement therapy during menopause, exposure to ionizing radiation, early age at first menstruation, and family history. Breast cancer is the most serious health problem in developed countries, proper diet, exercise and healthy life style can reduces the risk of breast cancer. Surgery and chemotherapy are the treatment for breast cancer, as chemotherapy has many side effects 50% weakness occurs due to cancer and 50% weakness occurs due to chemotherapy, hence herbal drugs or herbal treatment is the best option for breast cancer. Number of herbal drugs are effective in breast cancer In this study detailed study of the herbal drug used in breast cancer is discussed in detail.