Research Article of American Journal of Cancer Research and Reviews
BIOACTIVITY OF Caulerpa taxifolia (Vahl) Agardh (1817) IN METABOLISM AND VASCULOGENE OF TUMOR CELLS
Evandro Valentim da Silva1*, Eunice Ribeiro2, Wagnaer Mateus Vaz da Silva1, Dazziane Farias Santos1, Renê Rodrigues Melo2, Márcia Raquel Cedrim Vieira2, Danielle Maria Bastos de Souza3, José Ferreira Silva Neto3, George Chaves Jimenez3, Ivone Antonia de Souza1 and Fálba Bernadete Ramos dos Anjos1.
Federal University of Pernambuco1, Universitaria City, Recife, PE, Brazil. Northern College of Parana, Brazil2. University Federal Rural of Pernambuco, Brazil3.
Among many mechanisms, stimulation of angiogenesis leads to increased secretion of vaso-inducing factors and decreased inhibitors. The present study evaluated the interference of Caulerpa taxifolia hydroalcoholic (EHA – 50 μg / mL) and methanolic (EM – 50 μg / mL) extracts in energy metabolism and Angiogenesis of embryos of Gallus gallus domestic L. Rhode Island Red Eggs Red of four lots, were incubated at 37º C, with automatic turning, for the study of energy metabolism and angiogenesis. The groups were distributed and divided into control groups, treated EHA (50 μg / mL) and ME (50 μg / mL), and control enriched with ω-3, with and without tumor. In the 288 hour stage, development was halted. The vessels were quantified and characterized morphologically and the embryos photographed, fixed and processed. The results of energetic metabolism indicated that there was no significant difference between control and normal treated groups, however, animals with EHA and ME induced tumors and those with ω-3 enriched presented a significant response when compared to control (p≤0.05). It was observed that the physical properties and the structural integrity of the bark of the control animals showed formation abnormalities: rough, rough and soft bark. Morphometric parameters were not significant between groups (p≤0.05). In relation to vasculogenesis and angiogenesis there was a significant reduction between normal and tumorous groups. The vessels showed a slight reduction of the caliber when compared to the control group (p≤0.05). The microscopic appearance of the amniotic membrane of the organisms treated with EHA and ME from the normal and tumor groups maintained the morphology preserved throughout the treatment. Cardiovascular tissue from tumor-containing embryos had bleeding points and a congestion in the lumen of the vessel, perhaps due to the presence of malignant cells. Microscopy of the medulla was preserved. In the control embryos it was poorly conserved, with congested vessels in the mantle region, possibly due to interference of the tumor in the analyzed tissue. These biocomposites also had an anisculogenic and antiangiogenic effect, without compromising the macroscopic and microscopic anatomy of the organisms treated during the embryonic development. In view of the present, it is concluded that the compounds present in extracts of Caulerpa taxifolia can be considered a potential antitumor agent of marine origin, which may act on the intrinsic mechanisms related to the extracellular matrix, stabilizing and remodeling vascular structure and the capacity of induction of neovasculogenesis.
Keywords: Seaweed.Caulerpa taxifolia, Angiogenesis. Vasculogenesis. Antitumoral.
How to cite this article:
Evandro Valentim da Silva, Eunice Ribeiro, Wagnaer Mateus Vaz da Silva, Dazziane Farias Santos, Renê Rodrigues Melo, Márcia Raquel Cedrim Vieira, Danielle Maria Bastos de Souza, José Ferreira Silva Neto, George Chaves Jimenez, Ivone Antonia de Souza and Fálba Bernadete Ramos dos Anjos. .BIOACTIVITY OF Caulerpa taxifolia (Vahl) Agardh (1817) IN METABOLISM AND VASCULOGENE OF TUMOR CELLS.. American Journal of Cancer Research and Reviews, 2019,3:9. DOI:10.28933/ajocrr-2019-05-1105
1. ARGILÉS, J.M.; BUSQUETS, S.; LOPES-SORIANO, F.J. The pivotal role of cytokines in muscle wasting during cancer. The Int. J. of Biochemistry & Cell Biology. 37: 2036– 2046, 2005.
2. Wound Healing. Plastic and Reconstructive Sugery, 117, 12S – 34S, 2006.
3. BROUILLARD, P.; VIKKULA, M. Vascular malformations: localized defects in vascular morphogenesis. Clin Genet, v. 63, 340 – 351, 2003.
4. CAMPS, C., IRANZO, V., BREMNES, R.M., SIRERA, R. Anorexia-cachexia syndrome in cancer: implications of the ubiquitin-proteasome pathway. Support Care Cancer 14: 1173-1183, 2006.
5. CAPP, c., ZENNIG, N., WAJNER, S., MAIA, a. l. Papel do fator de crescimento endotelial vascular nos carcinomas de tireoide the role of vascular endothelial growth factor in tumor. Rev. HCPA 2009;29 (1):51-59.
6. COURNEYA, K., FRIEDENREICH, C.M. Relationship between exercise pattern across the cancer experience and current quality of life in colorectal cancer survivors. Journal of Alternative and Complementary Medicine 3: 215–226, 1997.
7. GRAÇA, B., LUNET, C., COELHO, A. S., MONTEIRO, G.; FREIRE, P., SPEIDEL, A., CARVALHO, L. ANGIOGÉNESE E CANCRO da biopatologia à terapêutica. Instituto de Anatomia Patológica. Faculdade de Medicina de Coimbra – Coimbra. ACTA MÉDICA PORTUGUESA 2004; 17: 76-93.
8. HIRATSUKA S, NAKAMURA K, IWAI S, MURAKAMI M, ITOH T, KIJIMA H, et al. MMP9 induction by vascular endothelial growth factor receptor-1 is involved in lung-specific metastasis. Cancer Cell. 2002;2 (4):289-300.
9. KOWANETZ, M.; FERRARA, N. Vascular Endothelial Growth Factor Signaling Pathways: Therapetic Perspective. Clinical Cancer Research, 12 (17), 5018 – 5022, 2006.
10. POLVERINI, P. J. Angiogenesis in health and disease: insights into basic mechanisms and therapeutic opportunities. Journal of Dental Education, Washington, v. 66, n. 8, p. 962-975, 2002.
11. RAVI D, RAMADAS K, MATHEW BS, NALINAKUMARI KR, NAIR MK, PILLAI MR. Angiogenesis during tumor progression in the oral cavity is related to reduce apoptosis and high tumor cell proliferation. Oral Oncol 1998; 34(6):543-8.
12. RODRIGUES, E. B.; ROSSI, E. E.; GRUMANN JUNIOR, A.; MEYER, C. H.; HO, A. C. Tratamento da forma neovascular de degeneração macular relacionada à idade com drogas antingiogênicas. Arquivos Brasileiros de Oftalmologia 5, 756 – 765, 2006.
13. SAKURAI E, ANAND A, AMBATI BK, VAN ROOIJEN N, AMBATI J. Macrophage depletion inhibits experimental choroidal neovascularization. Invest Ophthalmol Vis Sci. 2003;44(8):3578-85.
14. SANT’ANA, E. M.C. Estudo do efeito da Alternagina-C, uma desintegrina do veneno de Bothrops alternatus e de um peptídeo sintético derivado de sua estrutura, sobre a expressão de fatores de crescimento, angiogênese e cicatrização de lesão em pele de rato. Tese de Doutorado. Universidade Federal de São Carlos, São Carlos, 2008.
15. SATO, M.; TACHIBANA, T.; FURUSE, M. Heat production and lipid metabolism in broiler and layer chickens during embryonic development. Comparative Biochemistry and Physiology, v.143, p.382-388, 2006.
16. SHIBUYA, M. VEGF-VEGFR Signals in Health and Disease. Biomolecules and Therapeutics, Seoul, v. 22, n. 1, p. 1-9, 2014.
17. SOUZA GFM, FREITAS RA, MIRANDA JL. Angiogênese em carcinoma de células escamosas de língua e lábio inferior. Cienc Odontol Bras 2007;10(1):12-8.
18. SOUZA, W. F.; ARAÚJO, W. M.; de-FREITAS-JÚNIOR, J. C. M.; MORGADO-DÍAZ, J.A. Sinalização celular em câncer. Cienc. Cult. 66 (1), 30 – 33, 2014.
19. STOCKMANN, C.; SCHADENDORF, D.; KLOSE, R.; HELFRICH, I. The Impact of the Immune System on Tumor: Angiogenesis and Vascular Remodeling. Frontiers in Oncology, Lausanne, v. 4, n. p. 69, 2014.
20. STORKEBAUM E, CARMELIET P. VEGF: a critical player in neurodegeneration. J Clin Invest. 2004;113(1):14-8.
21. TAMMELA, T.; ENHOLM, B.; ALITALO, K. PAAVONEM The biology of vascular endotelial growth factors. Cardiovascular Research, 65, 550 – 563, 2005.
22. TAZAWA, H.; WHITTOW, G. C. Incubation physiology. In: WHITTOW, G. C. Sturkie’s Avian Physiology. 2000. Cap. 24, p.617-634.
23. TISDALE, M.J. Protein loss in cancer cachexia. Science 289:2293-2295, 2000.
24. USUI T, ISHIDA S, YAMASHIRO K, KAJI Y, POULAKI V, MOORE J, et al. VEGF164(165) as the pathological isoform: differential leukocyte and endothelial responses through VEGFR1 and VEGFR2. Invest Ophthalmol Vis Sci. 2004;45(2):36874.
25. ZETTER, B.R. “Angiogenesis and tumor metastasis”. Annual review of medicine. Palo Alto. 49:407-24, 1998.