Evandro Valentim da Silva1*, Eunice Ribeiro2, Wagnaer Mateus Vaz da Silva1, Dazziane Farias Santos1, Renê Rodrigues Melo2, Márcia Raquel Cedrim Vieira2, Danielle Maria Bastos de Souza3, José Ferreira Silva Neto3, George Chaves Jimenez3, Ivone Antonia de Souza1 and Fálba Bernadete Ramos dos Anjos1.

Federal University of Pernambuco1, Universitaria City, Recife, PE, Brazil. Northern College of Parana, Brazil2. University Federal Rural of Pernambuco, Brazil3.

American Journal of Cancer Research and Reviews

Among many mechanisms, stimulation of angiogenesis leads to increased secretion of vaso-inducing factors and decreased inhibitors. The present study evaluated the interference of Caulerpa taxifolia hydroalcoholic (EHA – 50 μg / mL) and methanolic (EM – 50 μg / mL) extracts in energy metabolism and Angiogenesis of embryos of Gallus gallus domestic L. Rhode Island Red Eggs Red of four lots, were incubated at 37º C, with automatic turning, for the study of energy metabolism and angiogenesis. The groups were distributed and divided into control groups, treated EHA (50 μg / mL) and ME (50 μg / mL), and control enriched with ω-3, with and without tumor. In the 288 hour stage, development was halted. The vessels were quantified and characterized morphologically and the embryos photographed, fixed and processed. The results of energetic metabolism indicated that there was no significant difference between control and normal treated groups, however, animals with EHA and ME induced tumors and those with ω-3 enriched presented a significant response when compared to control (p≤0.05). It was observed that the physical properties and the structural integrity of the bark of the control animals showed formation abnormalities: rough, rough and soft bark. Morphometric parameters were not significant between groups (p≤0.05). In relation to vasculogenesis and angiogenesis there was a significant reduction between normal and tumorous groups. The vessels showed a slight reduction of the caliber when compared to the control group (p≤0.05). The microscopic appearance of the amniotic membrane of the organisms treated with EHA and ME from the normal and tumor groups maintained the morphology preserved throughout the treatment. Cardiovascular tissue from tumor-containing embryos had bleeding points and a congestion in the lumen of the vessel, perhaps due to the presence of malignant cells. Microscopy of the medulla was preserved. In the control embryos it was poorly conserved, with congested vessels in the mantle region, possibly due to interference of the tumor in the analyzed tissue. These biocomposites also had an anisculogenic and antiangiogenic effect, without compromising the macroscopic and microscopic anatomy of the organisms treated during the embryonic development. In view of the present, it is concluded that the compounds present in extracts of Caulerpa taxifolia can be considered a potential antitumor agent of marine origin, which may act on the intrinsic mechanisms related to the extracellular matrix, stabilizing and remodeling vascular structure and the capacity of induction of neovasculogenesis.

Keywords: Seaweed.Caulerpa taxifolia, Angiogenesis. Vasculogenesis. Antitumoral.

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Evandro Valentim da Silva, Eunice Ribeiro, Wagnaer Mateus Vaz da Silva, Dazziane Farias Santos, Renê Rodrigues Melo, Márcia Raquel Cedrim Vieira, Danielle Maria Bastos de Souza, José Ferreira Silva Neto, George Chaves Jimenez, Ivone Antonia de Souza and Fálba Bernadete Ramos dos Anjos. .BIOACTIVITY OF Caulerpa taxifolia (Vahl) Agardh (1817) IN METABOLISM AND VASCULOGENE OF TUMOR CELLS.. American Journal of Cancer Research and Reviews, 2019,3:9. DOI:10.28933/ajocrr-2019-05-1105

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