The Effectiveness of Cream of Centella asiatica Ethanol Extract in Chitosan Nanoparticles Compared with Centella asiatica Ethanol Extract Cream, Silver Sulfadiazine Cream, and Control on Superficial Dermal Burn Healing in Rats


The Effectiveness of Cream of Centella asiatica Ethanol Extract in Chitosan Nanoparticles Compared with Centella asiatica Ethanol Extract Cream, Silver Sulfadiazine Cream, and Control on Superficial Dermal Burn Healing in Rats


Fifinela Raissa, Kusmarinah Bramono, Sondang P. Sirait, Lia Damayanti, Rafika Indah Paramita

Dermatology and Venereology Study Program, Faculty of Medicine, Universitas Indonesia


Introduction: Centella asiatica extract is known to promote wound healing. Nowadays nanotechnology, especially nanoparticle is used to increase the bioavailability of active ingredients into the skin, one of which is chitosan nanoparticle. However, the effectiveness of Centella asiatica ethanol extract in chitosan nanoparticle (CAEECN) towards superficial dermal burn healing is not fully understood.

Aim: To determine the effectiveness of CAEECN cream compared to Centella asiatica ethanol extract (CAEE) cream, silver sulfadiazine (SSD) cream and control in superficial dermis burn healing in Sprague-Dawley rats.

Materials and Methods: A total of 20 male Sprague-Dawley rats were divided into 4 groups. After anesthetizing, 4 superficial dermis burns were made on the back of each rat and was given 4 treatments (CAEECN, CAEE, SSD, and control) afterward. Each group was sacrificed separately (respectively on day 3rd, 7th, 14th, and 21st post-burn induction). Several evaluations were macroscopic (wound size), and microscopic parameters (reepithelialization ratio and distance, wound contraction index, wound size, and angiogenesis).

Results: There was no significant difference in wound size between treatments on day 0, 3, 7, 14, and 21. But three active agents (CAEECN, CAEE, and SSD) treated group showed early decrease of wound size compare to control group on day-3. Microscopically, there were no significant differences in reepithelialization ratio and distance, wound contraction index, wound size, angiogenesis. Nevertheless, CAEECN treated group showed the smallest wound size on day-3 and highest angiogenesis on day 21.

Conclusion: Effectiveness of CAEECN in burn wound healing in rats showed no significant difference compared to CAEE, SSD, and control. But in three active agents (CAEECN, CAEE, and SSD) treated group, showed an early decrease of wound size compared to control group on day-3. Microscopically, CAEECN treated group showed the smallest wound size on day-3 and highest angiogenesis on day 21.


Keywords: Wound healing, re-epithelialization, fibroblasts, angiogenesis, nanotechnology


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How to cite this article:
Fifinela Raissa, Kusmarinah Bramono, Sondang P. Sirait, Lia Damayanti, Rafika Indah Paramita.The Effectiveness of Cream of Centella asiatica Ethanol Extract in Chitosan Nanoparticles Compared with Centella asiatica Ethanol Extract Cream, Silver Sulfadiazine Cream, and Control on Superficial Dermal Burn Healing in Rats.American Journal of Dermatological Research and Reviews, 2020, 3:19. DOI: 10.28933/ajodrr-2020-01-1505


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