Histological and ultrastructural studies of myocardium lesions produced by hair dye (para-phenylenediamine) in rats


Histological and ultrastructural studies of myocardium lesions produced by hair dye (para-phenylenediamine) in rats


Aisha D. Alalwani

Department of Biology, Faculty of Sciences, University of Jeddah, Saudi Arabia.


American Journal of Histology and Cytology

Para-phenylenediamine (PPD) is an aromatic compound and usually applied in a few commercial and mechanical products. Additionally, ladies apply henna for coloring their hair, which contains PPD as one of the components. Henna is used in East Africa, India, and Middle Eastern countries as a part of their culture. However, it is rarely found in western countries. The aim of this study was to examine the effect of two different doses of PPD topical application on the skin of female rats. The microscopic analysis indicates that the body and heart weights were affected. Histopathological studies on cardiac fibers showed that the treatment of rats with 1.5 ml of PPD resulted in increased cytoplasmic vacuolization and the loss of myocardial cells with a globular nucleus. Additionally, an increased dose of 3 ml PPD showed several signs of cardiotoxic effects on increased cytoplasmic vacuolization and myofibrillar loss. Congestion, focal necrosis, swollen mitochondria, and lymphoid infiltration were also observed. Considering the findings of this study, it is concluded that the chronic usage of PPD promotes histopathological alterations in heart tissue of rats.


Keywords: Para-phenylenediamine, rats, myocardium, cardiac fibers, histopathology.

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How to cite this article:
Aisha D. Alalwani. Histological and ultrastructural studies of myocardium lesions produced by hair dye (para-phenylenediamine) in rats.American Journal of Histology and Cytology, 2019, 2:6. DOI: 10.28933/ajohc-2019-08-0705


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