Research Article of American Journal of Histology and Cytology
EVALUATION OF HEMOLYTIC ACTIVITY OF YANGAMBIN ISOLATED FROM Ocotea duckei VATTIMO-GIL
Maria Isabel de Assis Lima Castro1*, Marta Gerusa Soares de Lucena1,2, Camilla Vila Nova Soares Silva3, Eliete Cavalcanti da Silva1,2
1Postgraduate Program in Morphotechnology – Federal University of Pernambuco, 2Department of Histology and Embryology – Federal University of Pernambuco, 3Multiprofessional Residency Program in Health Surveillance – Recife Health Department
Yangambin, a lignan isolated from the leaves of Ocotea duckei Vattimo-Gil, has several pharmacological activities described in literature. However, few information about its toxicity has been reported. Red cells represent about 90% of blood cells and have interesting structural and molecular characteristics as experimental models in toxicological studies. Thus, the aim of this study was to evaluate the hemolytic action of yangambin in sheep blood. The hemolysis assay was performed at concentrations of 50, 25 and 12.5 µg/mL of yangambin in triplicate and hemolysis percentage was defined through the absorbance resulting from the test concentrations compared to the positive control. The results showed that yangambin did not cause hemolysis at the concentrations tested, therefore it did not cause damage to the plasma membrane of sheep erythrocytes.
Keywords: Hemolytic activity, cytotoxicity, Yangambin.
How to cite this article:
Maria Isabel de Assis Lima Castro, Marta Gerusa Soares de Lucena, Camilla Vila Nova Soares Silva3, Eliete Cavalcanti da Silva.EVALUATION OF HEMOLYTIC ACTIVITY OF YANGAMBIN ISOLATED FROM Ocotea duckei VATTIMO-GIL. American Journal of Histology and Cytology, 2021, 4:9. DOI: 10.28933/ajohc-2021-05-1805
1. Monte-Neto RL, Sousa LMA, Dias CS, Barbosa-Filho JM, Oliveira MR, Figueiredo RCBQ. Morphological and physiological changes in leishmania promastigotes induced by yangambin, a lignan obtained from Ocotea duckei. Exp Parasitol, 127 (2011) 215.
2. Algharably EAH, Kreutz R, Gundert-Remy U. Importance of in vitro conditions for modeling the in vivo dose in humans by in vitro–in vivo extrapolation (IVIVE). Archives of Toxicology, 1:1-7, 2019.
3. International Organization for Standardization (ISO). ISO 10993-5 Biological evaluation of medical devices – Tests for in vitro cytotoxicity. Stwitzerland; 2010.
4. Sarkar S, Bose D, Giri RP, Mukhopadhyay MK, Chakrabarti A. Status of Membrane Asymmetry in Erythrocytes: Role of Spectrin. Biochemical and Biophysical Roles of Cell Surface Molecules, 3–11, 2018.
5. Flood SL, Burkholder JM. Chattonella subsalsa (Raphidophyceae) growth and hemolytic activity in response to agriculturally-derived estuarine contaminants. Harmful Algae, V. 76: 66-79, 2018.
6. Souza TC, Guedes AS, Santos LCS. Estudo fotoquímico e avaliação in vitro da atividade hemolítica de extratos aquosos do Illicium verum utilizado para o tratamento dos sintomas clínicos da dengue. Revista Diálogos & Ciência (D&C), v. 2, n. 40 (17), p.141-157, 2017.
7. Barbosa-Filho JM, Vargas MRW, Silva IG, França IS, Morais LCSL, Cunha EVL, Silva MS, Souza MFV, Chaves MCO, Almeida RN, Agra MF. Ocotea duckei: Exceptional source of yangambin and other furofuran lignans. Anais da Academia Brasileira de Ciencias, 71: 231-238, 1999
8. Dacie JV; Lewis SM. Practical Haematology. 5th edition. Churchill Livingstone: Edinburgh, London, New York, 1975.
9. Aparicio RM, García-Celma MJ, Vinardell MP, Mitjans M. 2005. In vitro studies of the hemolytic activity of microemulsions in human erythrocytes. Journal of Pharmaceutical and Biomedical Analysis. 39: 1063-1067.
10. Dandapat S, Kumar AMP; Sinha MP. Therapeutic efficacy of Cinnamomum tamala (Buch.- Ham.) and Aegle marmelos (L.) leaf. Balneo Res J, 5 (2014) 113.
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