EVALUATION OF HEMOLYTIC ACTIVITY OF YANGAMBIN ISOLATED FROM Ocotea duckei VATTIMO-GIL


EVALUATION OF HEMOLYTIC ACTIVITY OF YANGAMBIN ISOLATED FROM Ocotea duckei VATTIMO-GIL


Maria Isabel de Assis Lima Castro1*, Marta Gerusa Soares de Lucena1,2, Camilla Vila Nova Soares Silva3, Eliete Cavalcanti da Silva1,2

1Postgraduate Program in Morphotechnology – Federal University of Pernambuco, 2Department of Histology and Embryology – Federal University of Pernambuco, 3Multiprofessional Residency Program in Health Surveillance – Recife Health Department


Yangambin, a lignan isolated from the leaves of Ocotea duckei Vattimo-Gil, has several pharmacological activities described in literature. However, few information about its toxicity has been reported. Red cells represent about 90% of blood cells and have interesting structural and molecular characteristics as experimental models in toxicological studies. Thus, the aim of this study was to evaluate the hemolytic action of yangambin in sheep blood. The hemolysis assay was performed at concentrations of 50, 25 and 12.5 µg/mL of yangambin in triplicate and hemolysis percentage was defined through the absorbance resulting from the test concentrations compared to the positive control. The results showed that yangambin did not cause hemolysis at the concentrations tested, therefore it did not cause damage to the plasma membrane of sheep erythrocytes.


Keywords: Hemolytic activity, cytotoxicity, Yangambin.

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How to cite this article:
Maria Isabel de Assis Lima Castro, Marta Gerusa Soares de Lucena, Camilla Vila Nova Soares Silva3, Eliete Cavalcanti da Silva.EVALUATION OF HEMOLYTIC ACTIVITY OF YANGAMBIN ISOLATED FROM Ocotea duckei VATTIMO-GIL. American Journal of Histology and Cytology, 2021, 4:9. DOI: 10.28933/ajohc-2021-05-1805


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