PATHOLOGICAL CHANGES IN THE RATS’ EYES AFTER THE ADMINISTRATION OF OIL MACHINERY FLUID THROUGH STEREOTAXIC SURGERY


Pathological Changes In The Rats’ Eyes After The Administration Of Oil Machinery Fluid Through Stereotaxic Surgery


Martha Lilia Tena-Suck1*, Alma Ortiz-Plata2, Joaquín Manjarrez3, Wilhelm Moreno4, Carmen Rubio4 , Francisca Fernández-Valverde2

1Instituto Nacional de Neurología y Neurocirugía. Departamento de Neuropatología. Ciudad de México. 2Instituto Nacional de Neurología y Neurocirugía. Laboratorio de Neuropatología experimental Ciudad de México. 3Instituto Nacional de Neurología y Neurocirugía. Laboratorio de formación Reticular. Ciudad de México. 4Instituto Nacional de Neurología y Neurocirugía.  Departamento de Neurofisiología Ciudad de México.


Purpose: To achieve a relationship between oil machinery fluid (OMF) and the damage this fluid produces in several eye structures. In neuro-oncology patients, we know there are several parasellar tumors; one of them is the craniopharyngiomas that can produce a cystic structure containing an oily material. It has been denominated as an oil machinery fluid (OMF); this fluid has not yet been widely studied. It is a widely held view that it produces toxic effects in the brain and other structures. This paper aims to see the toxicity of the OMF when administered directly in the brain and the changes produced in the rats’ eyes.

Methods: 30 Wistar rats were divided into three groups, control, sham and experimental; the oil machinery fluid was obtained directly from human patients during surgery. The oil machinery fluid was administered to the rat thalamus by stereotaxic surgery. The subjects were under observation after the surgery for five weeks and sacrificed once the observation period ended. Finally, immunohistochemistry was performed on tissue recovered from the eyes.

Results: We observed that in the experimental group, there was an increase in glucose levels, the coloration of the eyes changed to a pinkish color, the lenses changed opacity, there were histological changes in the retina, and a reduction of the diameter of the optic nerve in this group in comparison with the control group.

Conclusions: All the results observed in this model can be seen in human patients with craniopharyngiomas and diabetes. They are leading us to think that the oil machinery fluid alone can produce ocular changes by damaging several structures by the toxicity created by this fluid.


Keywords: Craniopharyngioma; oil machinery fluid; cyst structures; immunohistochemistry and Eye structures

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How to cite this article:
Martha Lilia Tena-Suck, Alma Ortiz-Plata, Joaquín Manjarrez, Wilhelm Moreno, Carmen Rubio. Pathological Changes In The Rats’ Eyes After The Administration Of Oil Machinery Fluid Through Stereotaxic Surgery. American Journal of Histology and Cytology, 2021, 4:13. DOI: 10.28933/ajohc-2021-10-2105


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