Albizia Lebbeck Stem Bark Aqueous Extract As Alternative To Antibiotic Feed Additives In Broiler Chicks Diets: Haematology, Serum Indices And Oxidative Status


Albizia Lebbeck Stem Bark Aqueous Extract As Alternative To Antibiotic Feed Additives In Broiler Chicks Diets: Haematology, Serum Indices And Oxidative Status


1*Alagbe, J.O., Ajagbe A.D2., Attama Jeremiah 3., Philemon, K.C4 and Bello, Kamoru. A5

1Sumitra Research Institute; 2Kogi State University; 3 University of Nigeria; 4 University of Abuja; 5Al-Hikmah University Ilorin


A total of Three hundred and seventy five (375) one day old (Ross 308) broiler chicks with mixed sex were used to examine the effects of Albizia lebbeck stem bark (ATSM) aqueous extract as alternative to antibiotic feed additives in broiler chicks diets: haematology, serum biochemical indices and oxidative status. Birds were divided to five treatments with five replicates of fifteen (15) birds in a completely randomized design. Treatment 1 (basal diet + 0 % ATSM), treatment 2 (basal diet +1.2 grams Oxytetracycline per litre of water), treatment 3 (basal diet + 10 ml ATSM per liter of water), treatment 4 (basal diet + 20 ml ATSM per litre of water) and treatment 5 (basal diet + 30 ml ATSM per liter of water) and the trial lasted for 56 days. Results on some haematological parameters revealed that red blood cell (RBC), pack cell volume (PCV), haemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), white blood cell (WBC) and its differentials were significantly ((P<0.05) different among the treatments. Total protein, glucose, urea, cholesterol, creatinine, aspartate aminotransaminase (AST) and alanine aminotransferase (ALT) were significantly ((P<0.05) affected by ATSM. Activities of superoxide dismutase (SDA), glutathione peroxidase (GPx), catalase (CAT) and malonyldialdehyde (MLA) were significantly influenced by ATSM (P<0.05). It was concluded that ATSM could be administered to broiler chicks at 30 ml/litre without any negative effect on the general performance of birds.


Keywords: Albizia lebbeck; broiler chicks; haematology; serum biochemical indices.

Free Full-text PDF


How to cite this article:
Alagbe, J.O., Ajagbe A.D., Attama Jeremiah, Philemon, K.C and Bello, Kamoru. A. Albizia Lebbeck Stem Bark Aqueous Extract As Alternative To Antibiotic Feed Additives In Broiler Chicks Diets: Haematology, Serum Indices And Oxidative Status.Advances in Research and Reviews, 2020, 1:12


References:

1. Teodora, P., Evgeni, P and Maya, I. (2020). Ef-fect of black soldier fly (Hermetia illucens) meals on the meat quality of broilers. Agriculture and Food Science. 29:177-188.
2. Aldi-Hachesoo, B., Talebi, A and Asri-Rezaei, S. (2012).Comparative study on blood profiles of indigenous and Ross-308 broiler breeders. Global Veterinary Journal 7:238-241.
3. Cho, J.H., Kim, H.J and Kim, I.H (2014). Effects of phytogenic feed additive on growth perfor-mance, digestibility, blood metabolites, intestinal microbiota, meat colour and relative weight after oral challenge with Clostridium perfringens in broilers. Livestock Science, 160:82-88.
4. Alagbe, J.O and Oluwafemi, R.A. (2019). Growth performance of weaner rabbits fed Noni (Morinda citrifolia) and Moringa olifera leaf mixture as par-tial replacement of soya bean meal. International Journal of Advanced Biological and Biomedical Research. 7(2): 185-195
5. Abdel-Wareth, A.A., Lohakare, J.D. (2014). Ef-fect of dietary supplementation of peppermint on performance, egg quality and serum metabol-ic profile of Hy-Line brown hens during the late laying period. Animal Feed Science and Tech-nology, 197:114-120.
6. Brosnan, J.T and Brosnan, M.E (2010). Creatine metabolism and the urea cycle. Journal of Mo-lecular Genetics and Metabolism. 100:S49–52.
7. Alagbe, J.O., Shittu, M.D and Eunice Abidemi Ojo (2020). Prospect of leaf extracts on the per-formance and blood profile of monogastric – A review. International Journal of Integrated Educa-tion. 3(7): 122-127.
8. Gupta, M., Mazumder, U., Umar, T., Gomathi, P and Kumar, R (2004). Antioxidant and hepato-protective effects of Bulhiniaracemosa against paracetamol and carbon tetrachloride induced liver damage in rats. Iran Journal of Pharmaco-logical Therapy 3:12-20.
9. Mc Donald, S.D., Prenzler, M and Robards, K (2001). Phenolic content and antioxidant activity of olive extracts. Journal of Food Chemistry 73:73-84.
10. Sharma, Y., Bilbis, L.S., Lawal, M., Hassan, S.W., Abbas, A.Y.I and Isezuo, S.A (2007). Acute and sub chronic toxicity studies of crude aqueous extract of Albizia chevalier. Asian Jour-nal of Biochemistry 2(4):224-236.
11. Labaran, I., Lukman, O., Afolabi, A.A., Jamil, D and Umar, M (2016). Analysis of some phyto-chemical and minerals found in aqueous stem bark extract of Albizia lebbeck. Dutse Journal of Pure and Applied Sciences. 2(1):231-237.
12. Gary, D.B and Richard, D.M (2002). Interrelation-ship between nutrition and immunity. VM 139 Se-ries at the Veterinary Medicine – Large Animal Clinical Sciences Dept, UF/IFAS Extension (2002).
13. Hollman, P.C. (2001). Evidence for health bene-fits of plant phenols: Local or systemic effects. Journal of Science Food Agriculture,s 81: 842-852.
14. Alagbe, J.O. (2020). Performance, hematology and serum biochemical parameters of weaner rabbits fed different levels of fermented Lage-naria brevifora whole fruit extract. Advances in Research and Reviews, 2020, 1:5.
15. Wu X, Ruan Z, Gao YL, Yin YL, Zhou XH, Wang L, Geng MM, Hou YQ, Wu G (2010). Dietary supplementation with L-arginine or N-carbamylglutamate enhances intestinal growth and heat shock protein-70 expression in wean-ling pigs fed a corn- and soybean meal-based diet. Amino Acids. 2010; 39:831–9
16. Ojewuyi, O.B, Ajiboye, T. O, Adebanjo, E. O, Ba-logun, A, Mohammed, A.O (2014). Proximate composition, phytochemical and mineral con-tents of young and mature Polyalthia longifolia Sonn.leaves Fountain Journal of Natural and Applied Sciences: 2014; 3(1): 10 – 19.
17. Baker D.H (2009). Advances in protein-amino ac-id nutrition of poultry. Amino Acids. 37:29–41.
18. Wu G, Bazer FW, Burghardt RC, Johnson GA, Kim SW, Knabe DA, Li P, Li XL, McKnight JR, et al. (2010).Proline and hydroxyproline metabo-lism: implications for animal and human nutrition. Amino Acids. Epub 2010 Aug 10.
19. Wu G, Bazer FW, Burghardt RC, Johnson GA, Kim SW, Knabe DA, Li XL, Satterfield MC, Smith SB, et al (2010). Functional amino acids in swine nutrition and production. In: Doppenberg J, edi-tor. Dynamics in animal nutrition. Wageningen (The Netherlands): Wageningen Academic Pub-lishers; p. 69–98.
20. McKnight JR, Satterfield MC, Jobgen WS, Smith SB, Spencer TE, Meininger CJ, McNeal CJ, Wu G (2010).Beneficial effects of L-arginine on re-ducing obesity: potential mechanisms and im-portant implications for human health. Amino Ac-ids. 39:349–57
21. Tan B, Yin Y, Kong X, Li P, Li X, Gao H, Li X, Huang R, Wu G. (2010). L-Arginine stimulates proliferation and prevents endotoxin-induced death of intestinal cells. Amino Acids. 38:1227–35.
22. Yin YL, Yao K, Liu ZJ, Gong M, Ruan Z, Deng D, Tan BE, Liu ZQ, Wu G.(2010). Supplementing L-leucine to a low-protein diet increases tissue protein synthesis in weanling pigs. Amino Acids. Epub 2010 May 15.
23. Palii S.S, Kays CE, Deval C, Bruhat A, Fafournoux P, Kilberg MS (2009). Specificity of amino acid regulated gene expression: analysis of gene subjected to either complete or single amino acid deprivation. Amino Acids. 37:79–88.
24. Marc Rhoads J, Wu G (2009). Glutamine, argi-nine, and leucine signalling in the intestine. Ami-no Acids. 37:111–22.
25. Kimura H (2010). Hydrogen sulphide: from brain to gut. Antioxidant Redox Signal. 12:1111–23.
26. Abdi-Hachesoo, B., Talebi, A and Asri-Rezaei, S. (2011). Comparative study on blood profile of indigenous and Ross-308 broiler breeders. Global Veterinary Journal, 7:238-241.
27. Fugdge, A.M. (2000). Laboratory Medicine: Avi-an and Exotic Pets. W.B Saunders Company, Philadelpia.
28. Islam, M.S., Lucky, N.S., Literak, I., Ahad, A., Rahman, M.M and Siddiui, M.S.I. (2004). Chang-es of haematological parameters of fayoumi, assil and local chickens reared in Sylhet region in Bangladesh. International Journal of Poultry Science, 3:144-147.
29. Obiakaonu, H.O., Okoli, I.C., Opara, M.N., Oko-ro, V.M.O., Ogbuewu, I.P., Etuk, E.B and Uded-ibie. A.B.I (2011). Haematological and serum bi-ochemical indices of starter broilers fed neem leaf meal. Online Journal of Animal and Feed Research, 1(4):150-154.
30. Subhadarsini, M and Silpa, M.G. (2020). Com-parative haematology and biochemical parame-ters of Indigenous broiler chicken. International Journal of Scientific Technology Research, 9(4):972-978.
31. Santi, D.U and Kim, I.H (2017). Efficacy of phy-togenic feed additive on performance produc-tion and health status of monogastric animals – A review. Annals of Animal Science. 17(4): 929-948.
32. Talebi, A., Asri-Rezaei, S., Rozeh-Chai, R and Sahraei, R. (2005). Comparative studies on hae-matological values of broiler strains (Ross, Cobb, Arbo-acres and Arian). International Jour-nal of Poultry Science, 4(8):573-579.
33. Livingston, M.L., Cowieson, A.J., Crespo, R., Hoang, V., Nogal, B and Browning, M. (2020). Effect of broiler genetics, age and gender on performance and blood chemistry. Heliyon 6 (2020) e 04400.
34. Omokore, E.O and Alagbe, J.O. (2019). Efficacy of dried Phyllantus amarus leaf meal as an herb-al feed additive on the growth performance, haematology and serum biochemistry of growing rabbits. International Journal of Academic Re-search and Development. 4(3): 97-104.
35. Obajuluwa, O.V., Sanwo, K.A., Egbeyale, L.T and Fafiolu, O.A. (2020). Performance, blood profile and gut morphometry of broiler chickens fed diets supplemented with Yohimbe (Pausynistalia yohimbe) and Larvacide. Journal of Veterianary and Animal Science
36. Mahipal, C., Ashak, K.P., Shalini, B., Narayan, D., Sunil, E.J and Kusumakar, S. (2015). Dietary supplementation of a novel phytogenic feed ad-ditive: effects on nutrient metabolism, antioxi-dant status and immune response of goats. An-imal Production Science, 2:21-28. http://dx.doi.org/10.1071/AN14770.
37. Olubukola, S.O., Anthony, J.A and Adewale, A (2015). Sub-chronic administration of methanolic whole fruit extracts of Lagenaria breviflora (Benth) induces mild toxicity in rats. Journal of Pharmacognosy and natural product. 11:516-521.
38. Alagbe, J.O. (2019). Haematology, serum bio-chemistry, relative organ weight and bacteria count of broiler chicken given different levels of Luffa aegyptiaca leaf extracts. International Jour-nal of Advanced Biological and Biomedical Re-search. 7(4):382-392.
39. Nse Abasi, N.E., Mary, E.W., Uduak, A and Edem, E.A.O (2014). Haematological parameters and factors affecting their values. Journal of Ag-ricultural Science, 2(1): 37-47.
40. Isaac, L. J., Abah, G., Akpan, B and Ekaette, I.U (2013). Haematological properties of different breeds and sexes of rabbits. Proceedings of the 18th Annual Conference of Animal Science As-sociation of Nigeria. (Pg. 24-27).
41. Uwaya, J.O., Okunrobo, L.O and Igbinaduwa, P.O (2016). Albizia zygia: A comparative investi-gation of phytochemical composition, proximate analysis and anti-seizure properties of methanol extracts and its leaves and stem bark. Journal of Phytochemistry 1(4): 23-27.
42. Ugwuene, M.C (2011). Effect of dietary palm kernel meal for maize on the haematology and serum chemistry of broiler turkey. Nigerian Jour-nal of Animal Science 13:93-103.
43. Lin J, Hunkapiller AA, Layton AC, Chang Y.J, Robbins KR (2003) Response of intestinal mi-crobiota to antibiotic growth promoters in chick-ens. Foodborne Pathog Dis 10:331–337.
44. Lan, PTN, le Binh T, Benno Y (2013). Impact of two probiotic Lactobacillus strains feeding on fecal lactobacilli and weight gains in chicken. J Gen Appl Microbiol 49:29–36
45. Oluwafemi, R.A., Isiaka Olawale and Alagbe, J.O. (2020). Recent trends in the utilization of medicinal plants as growth promoters in poultry nutrition- A review. Research in: Agricultural and Veterinary Sciences. 4(1): 5-11.
46. Alagbe, J.O. (2019). Growth performance and haemato-biochemical parameters of broilers fed different levels of Parkia biglobosa leaf extracts. Academic Journal of Life Sciences. 5(12): 107 – 115.
47. Abdel, J, Giallongo F, Frederick T, Pate J, Walusimbi S, Elias RJ, Wall EH, Bravo D, Hris-tov AN (2014) Effects of dietary Capsicum oleo-resin on productivity and immune responses in lactating dairy cows. J Dairy Sci 98:6327–6339
48. Cho, S, Alden N, Lee K (2014) .Pathways and functions of gut microbiota metabolism impact-ing host physiology. Current Opinion on Bio-technology. 36:137–145
49. Olafadehan, O.A., Oluwafemi, R.A and Alagbe, J.O. (2020). Performance, haemato-biochemical parameters of broiler chicks administered Rolfe (Daniellia oliveri) leaf extract as an antibiotic al-ternative. Advances in Research and Reviews, 2020, 1:4.
50. A.O.A.C.(2000). Association of Official Analyti-cal Chemists. Official Methods of Analysis 19th Edition Washington, D.C Pages 69-77.
51. Aduku, A.O (2004). Animal nutrition in the trop-ics: Feeds and feeding in monogastric and rumi-nant nutrition. Journal of Applied Poultry Re-search, 13: 628-638.
52. Duncan, D.B. (1955). Multiple range and multiple F-test. Biometrics 11(1):1-42.
53. National Research Council (1994). Nutrient re-quirement of poultry 9th Rev Edn, Washington D.C. National Academy Press.
54. Akande, T and Odunsi, A.A (2012). Nutritive val-ue and biochemical changes in broiler chickens fed detoxified castor kernel cake based diets. African Journal of Biotechnology, 11(12):2904-2911
55. OIabanji, R.O., GO Farinu., JA Akinlade., OO Ojebiyi., AA Odunsi and AA Akingbade (2007). Studies on Haematological and Serum Biochem-ical Characteristics of Weaner Rabbits Fed Dif-ferent Levels of Wild Sunflower (Tithonia diversi-folia Hemsl A. Gray) Leaf- Blood Meal Mixture. Internation Journal of Agriculture and Apiculture Research. 4 (1&2): 80-89.
56. Olafadehan, O.A., Oluwafemi, R.A and Alagbe, J.O (2020). Carcass quality, nutrient retention and caeca microbial population of broiler chicks administered Rolfe (Daniellia oliveri) leaf extract as an antibiotic alternative. Journal of Drug Dis-covery. 14(33):146-154.
57. Karuppannan, K (2013). Phytopharmacological properties of Albizia species: A review. Interna-tional Journal of Pharmacy and Pharmacological Sciences 10 (5): 3-9.
58. Mohammed, F., Singh, P.P and Irchhaiya, R (2012). Review on Albizia lebbecka potent herbal drugs. International Research Journal of Phar-macy 3(1): 63-68.
59. Alagbe, J.O and Soares, D.M. (2018). Effects of feeding different levels of Azolla pinnata, Polyal-thia longifolia, Tithonia diversifolia, Moringa olif-era, Azadiracta indica leaf meal infusion as an organic supplement on the performance and nu-trient retention of growing grass cutters. Greener Journal of Agricultural Sciences. 8(1):01-11.