DPPH Free Radical Scavenging Activity of Ethanolic Extracts of Twenty two Medicinal Species From South Algeria (Laghouat region)


DPPH Free Radical Scavenging Activity of Ethanolic Extracts of Twenty two Medicinal Species From South Algeria (Laghouat region)


Boulanouar BAKCHICHE* , Hadjira GUENANE, Maria REZZOUG and Abdelaziz GHERIB

Laboratoire de Génie des Procédés, Université Amar Telidji, Laghouat, Algeria


Global Journal of Energy and Environment

Free radicals scavenging Activity, total phenolic and flavonoids contents of Twenty two ethanolic extracts, from the botanical families Asteraceae, Lamiaceae, Amaranthaceae, Chenopodiaceae, Cupressaceae, Ericaceae and Rhamnaceae, collected from the Laghouat region (Algeria Sahara) were investigated. The 2,2-diphenyl-1-picrylhydrazyl radical assay was used to determine the antioxidant activity of the plant extracts, while the Folin–Ciocalteu method was used to determine the total phenolic content and flavonoids using AlCl3 method . The antioxidant capacity expressed as IC50 values ranged from 20 µg/ml for O. basilicum to 650 ± 8.60 µg/ml for A. iva. The total phenolic content ranged from 2.72 to 87.11 mg/g of dry weight of extract, expressed as gallic acid equivalents. The total flavonoid concentrations varied from 1.48 to 12.59 mg/g, expressed as rutin equivalents. The results of this study showed that there is no significant correlation between antioxidant activity and phenolic content of the studied plant materials and phenolic content could not be a good indicator of antioxidant capacity.


Keywords: Medicinal plants; Phenolic content; flavonoid content ; Antioxidant; DPPH.

Free Full-text PDF


How to cite this article:
Boulanouar BAKCHICHE, Hadjira GUENANE, Maria REZZOUG and Abdelaziz GHERIB. DPPH Free Radical Scavenging Activity of Ethanolic Extracts of Twenty two Medicinal Species From South Algeria (Laghouat region). Global Journal of Energy and Environment, 2019,1:3.


References:

1. Bakchiche B, Gherib A, Smail A, Custodia G, Graca M (2013). Antioxidant activities of eight Algerian plant extracts and two essential oils. Ind. Crops Prod. 46:85-96.
2. Hammoudi .R, Hajj Mahammed .M, Ramdane .F and Ouldelhadj .M. D. (2013).Antimicrobial activity of phenolic extract from Teucrium polium geyrii (Lamiaceae) plant. Pharmacognosy Communications.3 : 35-38.
3. Derwich, E.,Benziane, Z.,&Boukir, A. (2010). GC/MS analysis and antibacterial activity of the essential oil of Mentha pulegium grown in Morocco. Research Journal of Agriculture and Biological Sciences, 6(3),191–198.
4. Barkat M and Laib I, Antioxidant activity of the essential oil from the flowers of Lavandula stoechas.2012. J. Pharmacognosy Phytother., 4, 96-101.
5. Giweli A.A, Džamić A.M, Marina D. Soković, Mihailo S. Ristić, Petar D. Marin. 2013.Chemical composition, antioxidant and antimicrobial activities of essential oil of Thymus algeriensis wild-growing in Libya. Cent. Eur. J. Biol. 8(5), 504-511
6. Pop A.V, Tofană M, Sonia A , Vârban D, Nagy M , Borş M.D, Fărcaş A. 2015.Essential oil composition, phenolic content and antioxidant activity in Romanian Salvia officinalis L. Journal of Agroalimentary Processes and Technologies,21(3), 241-246
7. Znini M, Bouklah M, Majidi L, Kharchouf S ,Aouniti A, Bouyanzer A, Hammouti B, Costa J, Al-Dyab S.S. 2011. Chemical Composition and Inhibitory Effect of Mentha Spicata Essential Oil on the Corrosion of Steel in Molar Hydrochloric Acid . Int.J. Electrochem. Sci. 6, 691-697.
8. Touafek O. Nacer A. Kabouche A. Kabouche Z. and Bruneau. (2004). Chemical composition of the essential oil of rosmarinus officinalis cultivated in the algerian sahara. Chemistry of Natural Compounds, 40 : 28 – 29.
9. Akrout A, El Jani H, Amouri S, Neffati M (2010). Screening of antiradical and antibacterial activities of essential oils of Artemisia campestris L., Artemisia herba alba Asso and Thymus capitatus Hoff. Et Link. Recent Research in Science and Technology 2(1): 29-39
10. Sari, M., Sarri, D., Hendel, N., Boudjelal, A., 2012 .Ethnobotanical study of therapeutic plants used to treat arterial hypertension in the hodna region of Algeria. Glob. J. Res. Med. Plants Indig. Med.1,411–417.
11. Moussaoui, F., Alaoui, T., Aoudry, S., 2014. Census Ethnobotanical Study of Some Plants Used in Traditional Medicine in the City of Meknes. Am. J. Plant. Sci. 5, 2480–2496.
12. Boudjelal, A., Henchiri, C., Sari, M., Sarri, D., Hendel, N., Benkhaled, A., Ruberto, G., 2013. Herbalists and wild medicinal plants in M’Sila (North Algeria) : Anethnopharmacology survey. J. Ethnopharmacol. 148 : 395 – 402.
13. Djellouli, M., Moussaoui, A., Benmehdi, H., Ziane, L., Belabbes, A., Badraoui, M.,Slimani, N., Hamidi, N., 2013. Ethnopharmacological study and phytochemicalscreening of three plants (Asteraceae family) from the region of south west Algeria. Asian J. Nat. Appl. Sci. 2 (2), 159–165.
14. Sarri M, Mouyet F.Z, Benzianea M, Cheriet A., 2014.Traditional use of medicinal plants in a city at steppic character (M’sila, Algeria). Journal of Pharmacy & Pharmacognosy Research 2 (2), 31-35.
15. Meddour R and Meddour O.S .2015. Medicinal plants and their traditional uses in kabylia (Tizi- ouzou, Algeria). Arabian Journal of Medicinal and Aromatic Plants. 1(2) 137-151.
16. Elmhdwi M.F, Attitalla I.A and Khan B.A. 2015.Evaluation of Antibacterial Activity and Antioxidant Potential of Different Extracts from the Leaves of Juniperus Phoenicea.J Plant Pathol Microb 2015, 6:9-15
17. Dehliz L W, Acheuk F, Mlik R et al, (2016). Ethnobotanical study of some plants used in traditional medicine in the region of Oued Righ (Algerian Sahara). Journal of Medicinal Plants Studies; 4(2): 204-211.
18. Khacheba I, Djeridane A, Yousfi M. Twenty Traditional Algerian Plants Used i n Diabetes Therapy as Strong Inhibitors of -Amylase Activity. Hindawi Publishing Corporation. International Journal of Carbohydrate Chemistry, 2014;1-12.
19. Krimat S, Dob T, Lamari L, Boumeridja S, Chabane C, Metidji H. 2014.Antioxidant and antimicrobial activities of selected medicinal plants from Algeria. Journal of Coastal Life Medicine, 2(6): 478-483.
20. Djeridane,A., Yousfi,M., Nadjemi,B., Boutassouna,D., Stocker,P., &Vidal,N. (2006). Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry, 97 (4), 654 – 660.
21. Djidel S, Khennouf S, Baghiani A, Harzallah D, Arrar L.2009. Medicinal plants used traditionally in the Algerian folk medicine for gastrointestinal disorders and hypertension: total polyphenols, flavonoids and antioxidant activity. Acta Hortic;854:59-65.
22. Ouraïni D, Agoumi A, Ismaili -Alaoui M, Alaoui K, Cherrah Y, Amrani M, Belabbas M.A. 2005. Study of the activity of essential oils from aromatic plants antifungal properties in the different stages of development of dermatophytes ; Phytotherapy; 4: 147-157.
23. Muthu C, Ayyanar M, Raja N, and Ignacimuthu S. 2006. Medicinal plants used by traditional healers in Kancheepuram District of Tamil Nadu, India. Journal of Ethnobiology and Ethnomedicine, 2:43.
24. Boulos L. 1983. Medicinal plants of North Africa. Ed Reference Publications Inc Michigan.
25. Ali-Delille L. 2013. Les plantes médicinales d’Algérie. BERTI Edition, Alger, Algeria.
26. Ben Merabet, K., Abed, A., (1982): Quelques aspects de la pharmacopée traditionnelle algérienne. Le Pharmacien du Maghreb, 2: 18-28.
27. Brand-Williams, B., Cuvelier, M.E. and Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT, Food Science and Technology.28 :25-30.
28. Ahn, M.R., Kumazawa, S., Usui,Y., Nakamura,J., Matsuka,M., Zhu,F., et al., 2007. Antioxidant activity and constituents of propolis collected in various areas of China. Food Chem. 101, 1400–1409.
29. Slinkard, K., & Singleton, V.L. (1977). Total phenol analyses: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 28,49–55.