Research Article of International Journal of Animal Research
Molecular study for bovine herpes virus type 1 detection in Iranian cattle
Rassoul Hashemzehi1, Ayse Kilic2, Arman Akbarpour3, Esmaeil Mahmoudi3, Asghar Arshi4*
1. Department of Genetics, Fars Science & Research Branch, Islamic Azad University, Shiraz, Iran
2. Sivrice Vocational High School, University of Firat, 23119 Elazig-Turkey
3. Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
4. Young Researchers and Elite Club, Najafabad Branch, Islamic Azad University, Najafabad, Iran
Bovine herpes virus type 1 (BHV-1), the causative agent of infectious bovine rhinotracheitis, is a DNA virus. This pathogen is represents the most common viral pathogen found in cattle semen. The aim of the present study was to set up a of BHV-1 detection assay in bovine blood in Lorestan province using PCR assay. The blood samples of 285 cattle in Khoramabad, Azna, Aligoodarz, Borujerd and Poldokhtar were collected, total DNA was extracted and the region encoded the gI glycoprotein was amplified by PCR using specific primers. Out of 285 blood samples, 56 (19.64%) were positive for BHV-1 (468 bp). The highest and lowest frequencies of the bacterial infection were observed in Khoramabad and Borujerd cities with 21 and 12%, respectively. The results of this study demonstrated that PCR assay represent an excellent (suitable) alternative or additional tool for BHV-1 isolates detection. Finally the study revealed a high incidence of BHV-1 in the blood of Iranian cattle. Thus all cattle must be tested periodically for BHV-1 infection and antimicrobial drugs, to prevent BHV-1 occurrence in cattle must be used. The cattle must be free BHV-1 infection prior to use.
Keywords: Bovine herpes virus type 1, gI gene, PCR, Iran.
How to cite this article:
Rassoul Hashemzehi et al., Molecular study for bovine herpes virus type 1 detection in Iranian cattle. International Journal of Animal Research, 2017; 1:7. DOI:10.28933/ijar-2017-09-2601
1. Muylkens B, Thiry J, Kirten P, Schynts F, Thiry E. Bovine herpesvirus 1 infection and infectious bovine rhinotracheitis. Vet Res 2007; 38(2): 181–209.
2. Nandi S, Kumar M, Manohar M, Chauhan RS. Bovine herpes virus infections in cattle. Anim Heal Res Rev 2009; 10(1): 85–98.
3. Edwards S, White H, Nixon P. A study of the predominant genotypes of bovid herpesvirus 1 found in the U.K. Vet Microbiol 1990; 22(2–3): 213–223.
4. Miller JM, Whetstone CA, Van der Maaten MJ. Abortifacient property of bovine herpesvirus type 1 isolates that represent three subtypes determined by restriction endonuclease analysis of viral DNA. Am J Vet Res 1991; 52(3): 458–461.
5. Schudel AA, Carrillo BJ, Wyler R, Metzler AE. Infections of Calves with Antigenic Variants of Bovine Herpesvirus 1 (BHV-1) and Neurological Disease. J Vet Med Ser B Blackwell Publishing Ltd 1986; 33(1–10): 303–310.
6. Preston CM, Nicholl MJ. Induction of cellular stress overcomes the requirement of herpes simplex virus type 1 for immediate-early protein ICP0 and reactivates expression from quiescent viral genomes. J Virol American Society for Microbiology 2008; 82(23): 11775–11783.
7. Turin L, Russo S. BHV-1 infection in cattle: an update. Vet Bull. CAB International 2003; 73(8): 15–21.
8. Lata J, Kanani AN, Purohit JH, Joshi CG, Rank DN, Kumar V, Jain VK. Detection of bovine herpesvirus 1 (BHV-1) infection in semen of Indian breeding bulls by polymerase chain reaction and its characterization by DNA sequencing. Buffalo Bull. International Buffalo Information Center 2009; 28(2): 76–84.
9. Jones C, Chowdhury S. A review of the biology of bovine herpesvirus type 1 (BHV-1), its role as a cofactor in the bovine respiratory disease complex and development of improved vaccines. Anim Heal Res Rev 2007; 8(2): 187–205.
10. Metzler AE, Matile H, Gassmann U, Engels M, Wyler R. European isolates of bovine herpesvirus 1: a comparison of restriction endonuclease sites, polypeptides, and reactivity with monoclonal antibodies. Arch Virol 1985; 85(1–2): 57–69.
11. van Oirschot JT. Bovine herpesvirus 1 in semen of bulls and the risk of transmission: A brief review. Vet Q 1995; 17(1): 29–33.
12. D’Arce RC., Almeida R., Silva T., Franco A., Spilki F, Roehe P., Arns CW. Restriction endonuclease and monoclonal antibody analysis of Brazilian isolates of bovine herpesviruses types 1 and 5. Vet Microbiol 2002; 88(4): 315–324.
13. Dragos A, Adriana A, Gheorghe S. Detection of Bovine Herpesvirus Type 1 by PCR Assay. Bull Univ Agric Sci Vet Med Cluj-Napoca Vet Med 2010; 67(2): 23–27.
14. Bahari A, Gharekhani J, Zandieh M, Sadeghi-Nasab A, Akbarein H, Karimi-Makhsous A, Yavari M. Serological study of bovine herpes virus type 1 in dairy herds of Hamedan province, Iran. Vet Res forum an Int Q J Faculty of Veterinary Medicine, Urmia University, Urmia, Iran 2013; 4(2): 111–114.
15. Haji Hajikolaei MR, Ghorbanpoor M, Solaymani M. The prevalence of Mycobacterium paratuberculosis infection in ileocecal valve of cattle slaughtered in Ahvaz abattoir, southern Iran. Iran J Vet Res 2006; 7(2): 77–80.
16. Sakhaee E, Khalili M, Kazemi nia S. Serological study of bovine viral respiratory diseases in dairy herds in Kerman province, Iran. Iran J Vet Res 2009; 10(1): 49–53.
17. Badiei K, Ghane M, Mostaghni K. Seroprevalence of Bovine Herpes Virus Type 1 in the Industrial Dairy Cattle Herds in Suburb of Shiraz-iran. Aust J Basic Appl Sci 2010; 4(10): 4650–4654.
18. Hemmat Zade F, Momtaz H, Tajbakhsh E, Safari H. A serological survey on bovine hrpesvirus 1 (BHV-1) in ChaharMahal and Bakhtiari province, Iran. Pajouhesh Va Sazandegi 2002; 38–43.
19. Morshedi A, Mahmoudian A, Dalir B, Gharahkhani A, Rahmati R. Detection of anti BHV-1 antibody in milk and serum by ELISA, comparison using of milk and serum ELISA for determine of BHV-1 infection in cattle. J Vet Res 2003; 58(3): 257–259.
20. Nahida Laiju M, Kabir G, Islam M, Hasanuzzaman M, Rahman M. Chromosomal Variation in Two Species of Hordeum. Asian J Plant Sci 2003; 2(2): 224–227.
21. Mahmoud M, Ahmed S. Prevalence of Bovine Herpesvirus1 in Sheep and Goats in Egypt. Glob Vet 2009; 3(6): 472–479.
22. Santurde G, Da Silva N, Villares R, Tabares E, Solana A, Bautista JM, Castro JM. Rapid and high sensitivity test for direct detection of bovine herpesvirus-1 genome in clinical samples. Vet Microbiol 1996; 49(1–2): 81–92.
23. Vilcek S, Nettleton PF, Herring JA, Herring AJ. Rapid detection of bovine herpesvirus 1 (BHV 1) using the polymerase chain reaction. Vet Microbiol 1994; 42(1): 53–64.
24. Bosch JC, De Jong MC, Franken P, Frankena K, Hage JJ, Kaashoek MJ, Maris-Veldhuis MA, Noordhuizen JP, Van der Poel WH, Verhoeff J. An inactivated gE-negative marker vaccine and an experimental gD-subunit vaccine reduce the incidence of bovine herpesvirus 1 infections in the field. Vaccine 1998; 16(2–3): 265–271.
25. Rocha MA, Barbosa EF, Guedes RM, Lage AP, Leite RC, Gouveia AM. Detection of BHV-1 in a naturally infected bovine fetus by a nested PCR assay. Vet Res Commun 1999; 23(2): 133–141.
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