Research Article of International Journal of Animal Research
Relevance of probiotic, prebiotic and synbiotic supplementations on hemato-biochemical parameters, metabolic hormones, biometric measurements and carcass characteristics of sub-tropical Noemi lambs
S.F El-Mehanna1, M.M. Abdelsalam1, N.M. Hashem2, , K.E.M. El-Azrak2, M.M. Mansour3 and M.M Zeitoun1,2*
1Department of Animal Production and Breeding, College of Agriculture and Veterinary Medicine, Qassim University 51452, KSA
2Department of Animal and Fish Production, Faculty of Agriculture, Alexandria University, Alexandria, Egypt
3Department of Animal and Poultry Production, Faculty of Agriculture, Damanhour University, Egypt.
The effects of probiotic, prebiotic and synbiotic preparations on hemato-biochemical parameters, metabolic hormones, biometric measurements and carcass characteristics of growing lambs were studied. Twenty four growing Noemi male lambs were randomly allocated into 4 groups (n=6/group) in a complete randomized design and blocked according to their initial weights. Lambs in the first group were orally given 50 ml of physiological saline (0.9% NaCl) and served as control (CON), while lambs in other three groups were orally given 50 ml of aqueous dandelion extract (PRE) or fermented cow’s milk supplemented with lactic acid bacteria (PRO) or their mixture (1:1, SYN) every other day for 8 consecutive weeks. The results indicated that treatment had no effects on hematological parameters, except leukocytes number which was elevated (P<0.05) in all treated lambs compared to CON-lambs. Treatments had no effect on blood serum cholesterol, whereas treatment with PRO and SYN increased (P<0.05) blood serum glucose. Blood serum insulin and IGF-I concentrations were higher (P<0.05) in lambs given SYN than lambs in other groups. Different supplementations improved (P<0.05) finishing weight of lambs compared to control. There were no significant differences in carcass weight and dressing percentage among treatments. Values of most carcass measurements were not affected by treatment except a significant increase in chest depth in all supplemented lambs compared to control. Regarding to eye muscle area, as an indicator of tissue growth, there was a tendency of increase in the carcass of PRO and SYN lambs as compared to those of PRE and CON-lambs. Most offal weights were not affected by treatment, however a significant increase in weight of digestive tract was observed for PRO-group compared with CON, but other treatments did not show a significant effect. Yield of commercial cuts was not affected by treatment except for breast and loin cuts. The heaviest breast weight was found in carcass of lambs given PRO (P<0.05) and the heaviest weight of loin cut was in favor of those given SYN. The meat in carcass of control and PRE was lower than that of PRO and SYN with no significant differences. In conclusion, supplementing probiotics fermented cow’s milk to growing lambs improves their metabolic status and thus growth efficiency without a significant change in their carcass traits.
Keywords: Sheep, dandelion extract, lactobacillus bacteria, synbiotic, metabolism, carcass, hematology.
How to cite this article:
S.F El-Mehanna et al., Relevance of probiotic, prebiotic and synbiotic supplementations on hemato-biochemical parameters, metabolic hormones, biometric measurements and carcass characteristics of sub-tropical Noemi lambs. International Journal of Animal Research, 2017; 1:10. DOI:10.28933/ijar-2017-09-3001
1. Aattouri, N., Bouras, M., Tome, D., Marcos, A., Lemonnier, D., 2001. Oral ingestion of lactic acid bacteria by rats increases lymphocyte proliferation and interferon production. Brit. J. Nutr. 87, 367-373.
2. Abas , I., Kutay, H.C., Kahraman, R., Toker, N.Y., Özçelik, D., Ates, F., Kaçakci, A., 2007. Effects of organic acid and bacterial direct-fed microbial on fattening performance of Kivircik-male yearling lambs. Pakistan J. Nutr. 6 (2), 149-154.
3. Abdel-Salam, A.M., Zeitoun, M.M., Abdelsalam, M.M., 2014. Effect of synbiotic supplementation on growth performance blood metabolites, insulin and testosterone and wool traits of growing lambs. J. Biologic. Chem. 14, 292-298.
4. Abo El-Nor, S., Kholif, A., 1998. Effect of supplementation of live yeast culture in the diet on the productive performance of lactating buffaloes. Milchwissenschaft 53, 663-666.
5. Afolabi, K.D., Akinsoyinii, A.O., Olajide, R., Akinleye, S.B., 2010. Hematological parameters of the Nigerian local grower chickens fed varying dietary levels of palm kernel cake. Proc. of the 35th Annual Conf. of the Nig. Soc. for Anim. Prod., 247.
6. Alessawi, A.J.A., Al-Wazeer, A.A.M., 2001. Effect of addition of probiotic and black seeds to Awassi male lambs ration on some body and testes measurements. Kufa J. Vet. Med. Sci. 2, 12-19.
7. Anon, E.M., 2004. Application manual for APNAN countries. Future Tech Today. com. Effective Microorganisms, Application Guide. http://emfsafe.com/em/app.htm
8. Attia ,Y.A., El-Hanoun, A.M., Bovera, F., Monastra, G., El-Tahawy, W.S. Habiba, H.I., 2014. Growth performance, carcass quality, biochemical and hematological traits and immune response of growing rabbits as affected by different growth promoters. J. Anim. Physiol. Anim. Nutr. 98, 128-139.
9. Bar Barham, D., Trinder, P. , 1972. An improved color reagent for the determination of blood
10. Glu glucose by the oxidase system. Analyst 97(151), 142-145.
11. Bomba, A., Nemcova, R., Mudronova, D., Guba, P., 2002. The possibilities of potentiating the efficacy of probiotics. Trends Food Sci. Technol. 13, 121-126.
12. Chandra, R., Mehla, R.K., Sirohi, S.K., Rahman, H., 2009. Effect of probiotic supplementation on growth of crossbred calves. Ind. J. Anim. Sci. 79, 1254-1257.
13. Choi, U.K., Lee, O.H., Yim, J.H., Cho, C.W., Rhee, Y.K., Lim, S.I., Kim, Y.C.,.2010.
14. Hypolipidemic and antioxidant effects of dandelion (Taraxacum officinale) root and leaf on cholesterol-fed rabbits. Int. J. Mol. Sci. 11(1), 67-78.
15. Dobiri, N., Hajimohammedi, A., Mahdavi, A., 2016. Effect of different levels of BioSaf probiotic on performance, carcass characteristics and blood factors of Iranian Zandi lambs. J. Fisheries Livest. Prod., 4:2.
16. Feldman, B., Zinkl, J., Jain, N., 2000. Schalm’s veterinary hematology. Lippincott Williams and Wilkins Philadelphia, USA.
17. Gadekar, Y.P., Shinde, A.K.,Soren, N.M., Karim, S.A., 2014. Effect of different levels of lactobacillus acidophilus culture on carcass traits and meat quality of Malpura lambs. Ruminant Sci. 3, 229-234.
18. Gordon, T., Castelli, W.P., Hjortland, M.C., Kannel, W.B. and Dawber, T.R.1977. High density lipoprotein as a protective factor against coronary heart disease. The Framingham Study. Am. J. Med.62, 707-714.
19. Grela, E.R., Sobolewska, S., Roziński, T., 2014. Effect of inulin extracts or inulin-containing plant supplement on blood lipid indices and fatty acid profile in fattener tissues. Polish J. Vet. Sci. 17(1), 93-98.
20. Hashem, N.M., Abd-Elrazek, D., Abo-Elezz, Z.R., Latif , M.G.A., 2016. Effect of vitamin A or C on physiological and reproductive response of Rahmani ewes during subtropical summer breeding season. Small Rumin. Res. 144, 313-319.
21. Hillal, H., El-Sayaad, G., Abdella, M., 2011. Effect of growth promoters (probiotics) supplementation on performance, rumen activity and some blood constituents in growing lambs. Archiv. Tierzucht. 54, 607-617.
22. Hussein, A. F. (2014). Effect of biological additives on growth indices and physiological responses of weaned Najdi ram lambs. J. Exp. Biol. Agric. Sci. 2(6), 597-607.
23. Issakowicz, J., Bueno, M.S., Sampaio, A.C.K., Duarte, K.M.R., 2013. Effect of concentrate level and live yeast (Saccharomyces cerevisiae) supplementation on Texel lamb performance and carcass characteristics . Livest. Sci. 155,44-52.
24. Khalid, M. F., Shahzad, M. A., Sarwar, M., Rehman, A. U., Sharif, M., Mukhtar, N., 2011. Probiotics and lamb performance: A review. African J. Agricul. Res. 6, 5198-5203.
25. Kunavue, N., Lien, T.F., 2012. Effects of fulvic acid and probiotic on growth performance, nutrient digestibility, blood parameters and immunity of pigs. J. Anim. Sci. Adv. 2(8), 711-721.
26. Marai, I.F.M., El-Darawany, A.A., Fadiel, A., Abdel-Hafez, M.A.M., 2007. Physiological traits as affected by heat stress in sheep- a review. Small Rumin. Res. 71,1-12.
27. Meléndez, P. A. , Caprilesa, V. A. 2006. Antibacterial properties of tropical plants from Puerto Rico. Phytomedicine 13, 272-276.
28. Mukhtar, N., Sarwar, M., NISA, M.U., Sheikh, M.A., 2010. Growth Response of Growing Lambs Fed on Concentrate with or without Ionophores and Probiotics. Int. J. Agric. Biol. 12, 734-738.
29. Noori, M., Alikhani, M., Jahanian, R., 2016. Effect of partial substitution of milk with probiotic yogurt of different pH on performance, body conformation and blood biochemical parameters of Holstein calves. J. Appl. Anim. Res. 44, 221-229.
30. NRC. 2007. Nutrients requirements of sheep. Sixth Rev. Ed., pp.112.
31. Respondek, F., Gerard, P., Bossis, M., Boschat, L., Bruneau, A., Rabot, S., Wagner, A., Martin, J,C., 2013. Short-chain fructooligosaccharides modulate intestinal microbiota and metabolic parameters of humanized gnotobiotic diet induced obesity mice. Plos One 8 (8), e71026.
32. Saleh, A.A., Hayashi, K., Ijiri, D., Ohtsuka, A., 2014. Beneficial effects of Aspergillus awamori in broiler nutrition. World’s Poultry Sci. J. 70, 857-864.
33. Samanta A.K., Jayapal, N., Senani, S., Kolte, A.P., Sridhar, M., 2013. Prebiotic inulin: useful dietary adjuncts to manipulate the livestock gut microflora. Brazilian J. Microbiol. 44, 1-14.
34. Sarwar, F., Shahzad, M.A., Farooq, M. K., Nisa, M., 2011. Performance of growing lambs receiving altered plant protein sources with or without probiotics. Int. Conf. Asia Agric. Anim. 13 , 139-144.
35. SAS. 2001. Users Guide: Statistics. Version 8.2. Cary, North Carolina: SAS Institute Inc.
36. Schley, P.D., Field, C.J., 2002 .The immune-enhancing effects of dietary fiber and prebiotics. Br. J. Nutr. 87, 221-230.
37. Steel, R.G.D., Torrie, J.H., 1980. Principles and Procedures of Statistics. McGraw. Hill Book Company Inc. New York. USA.
38. Tamime, A.Y., Robinson, R.K., 1999. Yoghurt: Science and Technology. Wood head Publishers, Cambridge, pp 808.
39. Trebichavsky, I., Splichal, I., 2006 . Probiotics manipulate host cytokine response and induce antimicrobial peptides. Folia Microbiol. 51, 507- 510.
40. Wenk, C., 2003. Growth promoter alternatives after the ban antibiotics. Pig News Inform. 24, 11N-16N.
41. Whitley, N.C., Cazac, D., Rude, B.J., Jackson-O’Brien, D., Parveen, S., 2009. Use of a commercial probiotic supplement in meat goats. J. Anim. Sci. 87, 723-728.
42. Wilson, R.G., Smith, J.A., Yonts, C.D., 2004. Chicory root yield and carbohydrate composition is influenced by cultivar selection, planting and harvest date. Crop Sci. 44 ,748-752.
43. Yusuf, A.L., Goh Y.M. , Samsudin, A.A., Alimon, A.R., Sazili, A.Q., 2014. Growth performance, carcass characteristics and meat yield of Boer goats fed diets containing leaves or whole parts of Andrographis paniculata. Asian Austral. J. Anim. Sci. 27 (4), 503-510.
44. Zeitoun, M.M., Farahna, M., Al-Sobayil, K.A., Abdel-Salam, A.M., 2014. Impact of the aqueous extract of dandelion, probiotic and their synbiotic on male lamb’s testicular histopathology relative to semen characteristics. OJAS. 4, 23-30.