Research Article of International Journal of Animal Research
Gonadal, extra gonadal sperm reserve and daily sperm production of breeder cocks fed graded levels of dietary fumonisin B1
Ogunlade, Jacob Taiwo
Department of Animal Science, Ekiti State University, Ado-Ekiti, Nigeria.
Fumonisin B1 (FB1), a secondary metabolite of the fungus fusarium verticillioides is known to be consumed by farm animals and has been reported to be associated with various farm animal diseases. To account for potential reproductive effects \of fumonisin in cocks meant for breeding purpose, sixty pre-pubertal breeder cocks of about 16 weeks of age were randomly assigned to four diets containing 0.2, 5.2, 10.2 and 15.2mg FB1/kg constituting diets 1, 2, 3 and 4 respectively. After 16 weeks of feeding trial all the pubertal cocks were sacrificed. Their testes and epididimydes were carefully dissected, removed, weighed and recorded. The left and right organs were homogenized separately. Dietary FB1 did not significantly (p> 0.05) influence both the gonadal and extra gonadal weights of the cock. The gonadal sperm reserves (GSR) of cocks fed the control diet (5.54x 107/testis) was significantly superior (p<0.05) to those fed diet 4 (2.66 X 107/ testis). Expressing the GSR on per gram of testis basis, cocks fed with the control diet had a significantly higher value when compared with cocks fed diet 4. The GSR in the left testis both on per testis and gram of testis bases was superior to those of the right testis. The dietary FB1 levels significantly decreased the extra gonadal sperm reserves (ESR) of the cocks which ranged from 4.21X107/epididymis for cocks on diet 1 to 1.33X 107 /epididymis for cocks fed diet 4.
The daily sperm production (DSP) of the cocks both on per testis and per gram testis were significantly reduced as the inclusion levels of dietary FB1 increased. The DSP of cocks fed diet 4 were half the DSP of cocks fed the control diet. The study revealed that cocks intended for breeding purpose should not be exposed to dietary FB1 higher than 10.2 ppm FB1 for optimum reproductive performance.
Keywords: Gonadal sperm reserve, Extragonadal sperm reserve, Daily sperm production, Breeder cocks, Fumonisin.
How to cite this article:
Ogunlade, Jacob Taiwo.Gonadal, extra gonadal sperm reserve and daily sperm production of breeder cocks fed graded levels of dietary fumonisin B1. International Journal of Animal Research, 2019;3:22. DOI:10.28933/ijar-2018-11-2206
1. Bacon C.W., Porter, J.K., And Norred, W.E., 1995. Toxic interaction of fumonisin B1 and fusaric acid measured by injection into fertile chicken egg. Mycopathologia. 129, 29-35
2. Cerolini, S., Pizzi, F., Gliozzi,T., Maldjian, A., Zaniboni, L., And Parodi, L., 2003. Lipid manipulation of chicken semen by dietary means and its relation to fertility: a review. World’s Poultry Science Journal. 59, 11-17.
3. Danicke, S., Valenta, H., Klobasa, F., Doll, S., Ganter, M. and Flachowsky G., 2004. Effects of graded levels of fusarium toxin contaminated wheat in diets for fattening pigs on growth performance, nutrients digestibility, deoxynivarenol balance and clinical serum characteristics. Archives of Animal Nutrition 58:1-17.
4. Egbunike, G.N., 1979. The effects of micro doses of aflatoxin B1 on sperm production rates, epididymal sperm abnormality and fertility in the rat. Zbl.Vet. Med. 26: 66-72
5. Egbunike, G.N., And Elemo, A.O., 1978 Testicular and epididymal sperm reserve of cross breed european boars raised and maintained in the humid tropics. J. Reprod. Fert, 54,245-248
6. Egbunike, G.N., Holtz, W., Endell, W. and Smidth, D., 1975. Reproductive capacity of German Landrace boars: gonadal and extra gonadal sperm reserve . Zuchthygiene, 11,35-37
7. Egbunike, G.N., Nkanga, E,E.,1999. Effects of breed and season on the semen characteristics of the cock in the Humid Tropics. Trop. J. Anim. Sci. 1(1), 127-134.
8. Egbunike, G.N; 1995. A toast for spermatozoa: One half the story of mammalian life. An inaugural lecture. University of Ibadan, Ibadan, Nigeria Pp.43.
9. Floss, J.L., Casteel, S.W., Johnson, G.C., Rottinghaus G.E. and Krause G.F., 1994. Developmental toxicity of fumonisin in syrian hamsters. Mycopathologia. 128,33-38
10. Flynn, T.J., Pritchard, D., Bradlaw, J., Eppley, R. And Page, S., 1996. In Vitro embryotoxicity of fumonisin B1 evaluated with cultured postimplantation staged rat embryos. In Vitro Toxicology, 9, 271-279
11. Gbore, F.A., 2009. Reproductive organs weights and semen quality of pubertal boars fed dietary fumonisin B1. Animal. 3(8) 1133-1137
12. Hafez, E.S., 1993. Physiology of reproduction in poultry. In: Reproduction in farm animals 6th ed. E.S.E. Hafez(Ed). Published By Lea and Febiger, Philadephia 385-402
13. Harrison, L.R., Colvin, B.M., Greene, J.T., Newman, L.E., Cole, J.R.Jr., 1990. Pulmonary oedema and hydrothorax in swine produced by fumonisin B1, a toxic metabolite of Fusarium moniliforme. J.Vet. Diagn. Invest. 2, 217-221.
14. Hendricks, K. 1999. Fumonisin and neural tubes defects in South Texas. Epidemiology. 10, 198-200
15. Igboeli, G. And Rakha, A.M., 1971. Gonadal and extra gonadal sperm reserves of Indigenous Central African Bulls. J.Reprod. Fert 25:107-109
16. Javed, T., Richard, J.L., Bennet, G.A., Dombrink-Kurtzman, M.A., Bunte, R.M., Koelkebec, K.W., Cote, L.M., Leeper, R.W., Buck, W.B., 1993. Embryophatic and embryocidal effects of purified fumonisin B1 or Fusarium proliferatum culture material extract on chicken embryos. Mycopathologia. 123,185-193
17. Maldjian, A., Penny, P. and Noble, R., 2002. DHA-Rich marine oils and improved reproductive efficiency in pigs. Book of Abstracts. Ann. Meeting of the Amer. Oil Society (A.O.C.S), Montreal, Canada.
18. Murphy, P.A., Rice, L.G., Ross, P.F, 1993. Fumonisin B1, B2 and B3 content of Iowa, Wisconsin and Illinois corn and corn screening. J. Agric. Food Chem. 41, 263-266
19. Nelson, P.E., Juba, J.H., Ross, P.F., and Rice, L.G., 1994. Fumonisin production by Fusarium species on solid substrates. J. of Ass. of Off. Anal. Chem. Int. .77,522-524.
20. Nelson, P.E:,Platner, R.D., Shackelford, D.D., Desjardins, A.E., 1991. Production of fumonisin By Fusarium moniliforme strains from various substrates and geographic areas. App. Environ.Microbiol 57, 2410-2412
21. Nkanga, E.E., 1989. Breed and seasonal influences on the reproductive potential of the cock in humid tropical environment. Ph.D. Thesis, Animal Science Department, University Of Ibadan, Nigeria
22. Ogunlade, J.T. Ewuola, E.O. Gbore, F.A., Bandyopadhyay, R., Niezen, J., Egbunike, G.N.,2006. Testicular and epidydimal sperm reserves of rabbits fed fumonisin contaminated diets. W.Applied Sci.J. I (1), 35-38
23. Ogunlade, J.T., 2015. Effect of dietary fumonisin B1 on reproductive organs and semen quality indices of breeder cocks. Journal of Biology, Agriculture and Health Care Vol.6, 28-33.
24. Oluyemi, J.A., And Roberts, F.A., 2000. Poultry production in warm wet climates, 2nd ed. Spectrum Books Limited, Ibadan, Nigeria.Pp.1-7
25. SAS Institute Inc., 1999. Sas/Stat Users Guide. Version 8 for Windows SAS Institute Inc.
26. Shepherd, G.S., Thiel, P.G., Stockenstrom, S., Sydenham, E.W., 1996. Worldwide survey of fumonisin contamination of corn and corn based products. J. Assoc.Of. Anal. Chem. Int.79 ,671-687
27. Surai, P.F., Noble, R.C., Sporks, N.H.C. and Speake, B.K. 2000. Effect of long term supplementation with arachidonic or docosahexanoic acids on sperm production in the broiler chicken. Journal of Reproduction and Fertility. 120, 257-267
28. Swierstra, E.E., 1970. The effect of low ambient temperature on sperm production, epididymal sperm reserves and semen characteristics of boars. Biol. Reprod. 2: 23-28