Evaluation of the antioxidant activity of extracts obtained from the seaweed Caulerpa taxifolia


Evaluation of the antioxidant activity of extracts obtained from the seaweed Caulerpa taxifolia


Evandro Valentim da Silva1*, Romário Correia dos Santos2, Jose Ferreira Silva Neto3, George Chaves Jimenez4, Ivone Antônia de Souza5; Fálba Bernadete Ramos dos Anjos6

1Programa de Pós-Graduação em Ciências Farmacêuticas da Universidade Federal de Pernambuco UFPE; 2Graduando do Curso de Odontologia da Universidade Federal de Pernambuco – UFPE. 3Programa de Pós-Graduação em Química Fundamental da Universidade Federal de Pernambuco UFPE; 4Professor de Fisiologia Animal da Universidade Federal Rural de Pernambuco- UFRPE; 5Professora do Programa de Pós-Graduação em Ciências Farmacêuticas da Universidade Federal de Pernambuco – UFPE; 6Professora do Departamento de Histologia e Embriologia – UFPE


International Journal of Bioscience and Medicine

This study aimed to evaluate the antioxidant activity of the extracts of marine seaweed Caulerpa taxifolia by two methods of study of antioxidant activity. The antioxidant activity of the extracts hidroalcolico, methanol and hidroalcolico was evaluated according to Mitsuda (1967). The ability of the extracts to kidnap hydrogen peroxide was determined by Ruch method (1989). The analysis carried out by reading the samples in octoplicata in spectrophotometer, it was possible to calculate the antioxidant and scavenging activities of the extracts hydrogen peroxide. The antioxidant activity presented by the three extracts from C. taxifolia by the ferric thiocyanate method varied each other, showing the methanol extract greater activity than the alcohol and aqueous extracts hydro, may be due to the presence of secondary metabolites like terpenoids. The kidnapper power of hydrogen peroxide of Caulerpa taxifolia strata known significant values when compared to the reference drug.


Keywords:Green algae, Ferric thiocyanate, Hydrogen peroxide.

Free Full-text PDF


How to cite this article:
Evandro Valentim da Silva, Romário Correia dos Santos, Jose Ferreira Silva Neto, George Chaves Jimenez, Ivone Antônia de Souza; Fálba Bernadete Ramos dos Anjos, Evaluation of the antioxidant activity of extracts obtained from the seaweed Caulerpa taxifolia. International Journal of Bioscience and Medicine, 2019; 3:9. DOI: 10.28933/ijbm-2019-05-1805.


References:

1. ARTEEL, G.E., SIES, H., 2001. The biochemistry of selenium and glutathione system. Environ Toxicol Pharmacol. 10: 153–8.
2. AMICO, G.C., RODRÍGUEZ-CABAL, MA. and AIZEN, MA., 2009. The potential key seed-dispersing role of the arboreal marsupial Dromiciops gliroides. Acta Oecologica, vol. 35, p. 8-13.
3. ANDRADE S.F.; LEMOS M.; COMUNELLO E.; NOLDIN V.F.; CECHINEL-FILHO V.; NIERO R. 2007. Evaluation of the antiulcerogenic activity of Maytenus robusta (Celastraceae) in different experimental ulcer models. J. Ethnopharmacol 113: 252-257.
4. ABAD, M.J.; BERMEJO, P. Baccharis (Compositae): a review uptade. Arkivoc, v.7, p.76-96, 2007.
5. BLUNT, J.W.; COOP, B. R.; MUNRO, M.H.G.; NORTHCOTE, P.T.; PRINSEP, M.R. Marine natural products. Nat Prod. Rep., v.22, p. 15-61, 2005.
6. BLUNT, J.W.; COOP, B. R.; MUNRO, M.H.G.; NORTHCOTE, P.T.; PRINSEP, M.R. Marine natural products. Nat Prod. Rep., v.23, p. 26-78, 2006.
7. DENISOV, E.; DENISOVA, T. (2009). The Reactivity of Natural Phenols. Russ. Chem. Rev., Vol. 78, No. 11, 1047-1073.
8. JOLY, A.B. Botânica: introdução a taxonomia vegetal. 13ª edição, São Paulo companhia editora Nacional, volume 4, 420-428p.2005.
9. GHOSH, P.; ADHIKARI, U., GHOSH, P.K., PUJOL, C.A., CARLUCCI, M.J., DAMONTE, E.B.; RAY, B. (2004). In vitro anti-herpetic activity of sulfated polysaccharide fractions from Caulerpa racemosa. Phytochem. 65 (23): 3151-3157
10. MATSUKAWA,R.; DUBINSKY,Z.; KISHIMOTO,E.; MASAKKI,K.; MASUDA,Y.; TAKEUCHI,T.A. Comparison of screening methods for antioxidants activity in seaweeds. J. appl.Phycol, v. 9, p.29-35, 1997.
11. MITSUDA,H., YAMAMOTO.A., AND KUSANO,T. (1967). Agric.biol.chem. 31,115-118
12. MOURA,C.A. ; SILVA, E. V. ; CAVALCANTI, K.P.S. ; ANJOS, F. B. R. ; JIMENEZ, G. C.; OLIVEIRA,S.F. ; SOUZA,I.A. Perfil Fitoquimico da Macroalga Marinha Caulerpa taxifolia (M.VAHL) C. AGARDH, 1817 (CAULERPACEAE). In: Congresso Brasileiro de Oceanografia, 2012, Rio de Janeiro. Congresso Brasileiro de Oceanografia. Rio de Janeiro: UERJ, 2012. v. )1. p. 01-01.
13. MOZZACHIODI, R. et al. (2001) Caulerpenyne, a toxin from the seaweed Caulerpa taxifolia, depresses after polarization in invertebrate neurons Neuroscience.107, 519-526.
14. NEGRI, M.L.S.; POSSAMAI, J.C.; KAKASHIMA, T. atividade antioxidante das folhas de espinheira –santa- Mayteunus ilicifolia Mart. Ex Reiss. Secas em diferentes temperaturas. Rev.bras. farm. 19(2B):553-556, Abr.jun.2009.
15. OLIVEIRA, E.C. The seaweed resources of Brazil. In: CRITCHLEY, A.T.; OHNO, M., eds. Seaweed resources of the world. Yokosuka: Japan International Cooperation Agency, 1998. p.367-371.
16. KAHARAMAN, A., ERKASAP, N., KOKEN, T., SERTESER, M., AKTEPE, F., ERKASAP, S., 2003. The antioxidative and antihistaminic properties of quercetin in ethanolinduced gastric lesions. Toxicology 183, 133–142.
17. RUCH, R.J., CHENG, S.J., AND KLAUNIG, J.E. (1989) Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis, 10, pp. 1003-1008.
18. RAMARATHNAM, N. OSAWA, T.; NAMIKI, M.; TASHIRO, T. Studies on the relaionship between antioxidative activity of Rice hull and germination ability of rice seeds. J. Sci. Food Agric., v. 37, p.719-726, 1986.
19. RAMARATHNAM, N. OSAWA, T. OCHI, H. KAWAKISHI, S. The contribution of plant food antioxidants to human health. Trends Food Sci. Technol., v.6, p.75-82, 1995.
20. RAVEN P.H., EVERT R.F.; EICHHORN S.E. 2007. Biologia Vegetal. 7ª ed. Guanabara
21. Koogan, RJ. REVIERS, B. de. 2006. Biologia e Filogenia das Algas. 1ª ed. Editora Artmed, Porto Alegre.
22. ROESLER, R.; MALTA, L.G.; CARRASCO, L.C.; HOLANDA, R.B.; SOUZA, C.A.S.;
23. PASTORE, G.M. Atividade Antioxidante de frutas do cerrado. Ciênc. Tecnol. Aliment, Campinas, v.27, n.1, p.53-60, jan.-mar., 2007.
24. MAITI, B.C., THAMSON, R.H., MAHENDRAN M.1978.The structure of caulerpina, a pigment from Caulerpa algae. J. Chem.Res. (S) 1978: 126-127.
25. MATSUBARA, K., MATSUURA, Y., BASIC, A., LIAO, M.L., HORI, K.; MIYAZAWA, K. (2001). Anticoagulant properties of a sufated galactan preparation from a marine green alga, Codium cylindricum. Biol. Macrom. 28(5): 395-399.
26. SCHWEDE, J.G, CARDELLINA, II J.H., GRODE, S.H, JAMES Jr., T.R, BLACKMAN, A. J. 1987. Distribuition of the pigment caulerpin in species of the green alga Caulerpa. Phytochem. 26: 155-158.
27. VEST, S.E, DAWES, C.J, AND ROMEO, J.T. 1983. Distribuition of caulerpin and caulerpicin in eight species of the green algae Caulerpa (Caulerpales). Bot. Mar. 26:313-316.
28. VULCAI, N.E.; GOUPY, P.; CARIN-VEYRAT, C.; DANGLES, O. (2005) inibition of the metmyoglobin induced peroxidation in linoleic acid by dietary antioxydant: action in the aqueosus phase vs. lipid phase. Free radical research, 35, p.547-563.
29. HAYASE, F.; KATO, H. Antioxidative components of sweet potates. J. Nutr. Sci. Vitaminol., v.30, p.37-46, 1984.
30. HAYM.E; FENICAL.W, 1988; Marine plant-herbivore interactions. The ecology of Chemical defense, Ann. Ver. Ecol. Syst. n° 19, pp. 111-145.
31. RAYMUNDO, M.S.; HORTA, P.; FETT, R. Atividade antioxidante in vitro de extratos de algumas algas verdes (Chlorophyta) do litoral catarinense (Brasil). Rev. Bras. Cienc. Farm., v. 40, p. 495-503, 2004. 60.
32. RODRIGUES, J.A.G.; FARIAS, W.R.L. (2005). Extração, fracionamento, purificação e atividade anticoagulante dos polissacarídeos sulfatados da alga marinha verde Caulerpa racemosa (Caulerpales, Chlorophyta). In: Congresso Brasileiro de Engenharia de Pesca (pp. 1693-1701). Fortaleza: CD-Room do CONBEP, 14.
33. REN, X, YANG, L, LIU, J, SU, D, YOU, D, LIU, C., 2001. A novel glutathione peroxidase mimic with antioxidant activity. Arch Biochem Biophys, 25: 250.
34. SOLÈ-CAV, A.M.; KELECOM. A ,1988, Dialogo quimico dos mares, Ciência Hoje, n° 46, vol. 8, pp.18-29.
35. SOUZA, T.M.; SEVERI, J.A.; SILVA, V.Y.A; SANTOS, E.; PIETRO, L.C.L.R. Bioprospecção de atividade antioxidante e antimicrobiana da casca de Strtphnodendron adstringens (Mart.) Coville (Leguminosae – Mimosoidae). Rev. Ciênc. Farm. Básica Apl., v.28, n.2, p.221-226, 2007.
36. SZE, P. (1997). A biology to the algae. New York: McGraw-Hill.
37. XUE, C-H, YU G, HIRATA, T, TERAO, J, LIN, H. 1998. Antioxidative activities of several marine polysaccharides evaluated in a phosphatidylcholine-liposomal suspension and organic solventes. Biosci Biotech Bioch 62: 206-209.
38. XUE, C-H, FANG, Y, LIN, H, CHEN, L, LI, Z-J, DENG, D, LU, C-X. 2001. Chemical characters and antioxidative properties of sulfated polysaccharides from Laminaria japonica. J. Appl Phycol 13: 67-70.
39. YESILYURT, V. et al. Antioxidant potential and phenolic constituents of Salvia cedronella. Food Chemistry, v. 108, p. 31-39, 2008.