Research Article of International Journal of Food and Nutrition Research
HPLC profiling, in vitro antisickling and antioxidant activities of phenolic compound extracts from black bean seeds (Phaseolus vulgarus L.) used in the management of sickle cell disease in the West Region of Cameroon
Nkenmeni D. C.1, Kotue T. C.1*; Kumar P.2; Djouhou F. M.1 ;Ngo S. F.3; Pieme A. C.4; Kansci G1; Fokou E.1; Arumugam N.2
1Laboratory for Food Science and Metabolism – Department of Biochemistry – Faculty of Science – University of Yaounde 1, Cameroon; 2Department of Biotechnology – University of Pondicherry, India;3Hematological Service at the Central Hospital, Yaounde, Cameroon.; 4Laboratory of Biochemistry, Physiology and Pharmacology – Faculty of Medicine and Biomedical Science / UHC– University of Yaounde 1, Cameroon.
Natural molecules from food have been used to manage sickle cell crises. As a genetic blood disorder, treatment is complex and expensive. This study was carried out to establish the phenolic compounds profile of black bean seeds (Phaseolus vulgarus. L) commonly used by some families in the Western Region of Cameroon to manage sickle cell disease and to evaluate their in vitro antisickling, membrane stability and antioxidant properties. Free, bound and total phenolic contents were estimated to be 0.1±0; 0.108±0 and 0.212±0 mg EAG/g of sample respectively. Free phenolic compounds contain ferulic acid (0.013 μg/g), while bound phenolic compounds contain gallic acid (2.13 μg/g) and ferulic acid (0.037 μg/g). Free phenolic compounds had the higher rates of inhibition (82.26±2%), reversibility (69.86±3%) of sickling and the best effect on membrane stability of erythrocytes. Phenolic extracts from black bean seeds also showed a high global antioxidant activity with free phenolic compounds (28.42± 0 mgFeII/100g). Total phenolic compounds showed a better activity on DPPH radical with a IC50 of 2.42±1µg/µL while free phenolic compounds showed a better activity on scavenging hydroxyl radical with a IC50 of 1.5±0.5µg/µL. These results may justify the use of black bean seeds by sickle cell patients from Cameroon.
Keywords: Antioxidant, antisickling, black bean seeds, HPLC, phenolic compounds, sickle cell disease.
How to cite this article:
Nkenmeni D. C., Kotue T. C.; Kumar P.; Djouhou F. M.;Ngo S. F.; Pieme A. C.; Kansci G; Fokou E.; Arumugam N.. HPLC profiling, in vitro antisickling and antioxidant activities of phenolic compound extracts from black bean seeds (Phaseolus vulgarus L.) used in the management of sickle cell disease in the West Region of Cameroon.International Journal of Food and Nutrition Research, 2019; 3:30. DOI:10.28933/ijfnr-2019-08-0105
1. Beninger CW, Hosfield GL. 2003. Antioxidant Activity of Extracts, Condensed Tannin fractions and pure flavonoids from Phaseolus vulgaris L. seed coat color genotypes. Journal of Agriculture and Food Chemistry. 51: 7879-7883.
2. Cardador-Martinez G, Loarca-Pina GB and Oomah. 2002. Antioxidant activity in common beans (Phaseolus vulgaris L.). Joumal of Agriculture and Food Chemistry. 50: 6975-6980.
3. Heimler D, Vignolini P, Giulia DM, Romani A. 2005. Rapid tests to access the antioxidant activity of Phaseolus vulgaris L. dry beans. Joumal of Agriculture and Food Chemistry.53: 3053-3056.
4. Tatum VL, Chow CK. 1996. Antioxidant status and susceptibility of sickle erythrocytes to oxidative and osmotic stress. Free Radical Research.vol. 25, no. 2: pp. 133–139.
5. WHO. Sickle-cell anaemia – Report by the Secretariat. 2011.
6. Mpiana PT, Mudogo V, Tshibangu DST, Kitwa EK, Kanangila AB, Lumbu JBS, Ngbolua KN, Atibu EK and Kakule MK. 2008. Antisickling activity of anthocyanins from Bombax pentadrum, Ficus capensis and Ziziphus mucronata: Photodegradation effect. Journal of Ethnopharmacology. 120: 413-418.
7. Ogoda OJ, Akubue PI and Okide GB. 2002. The kinetics of reversal of pre-sickled erythrocytes by the aqueous extract of Cajanuscajan seeds. Phytother. Res. 6(8): 748-50.
8. Ramde-Tiendrebeogo I, Alphonsine R, Andre T, Adama H, Marius L, Hassanata M, Odile G, Nacoulma, Innocent P. 2012. Antioxidative and antibacterial activities of phenolic 1.compound from FicussurForssk and Ficussycomorus L. (Moraceae) :ptential for sickle cell disease treatment in Burkina faso Int. J. Biol. Chem. Sci. 6(1): 328-336.
9. Kotue TC, Pieme AC and Fokou E. 2016. Ethnobotanicals usages in the management of sickle cell disease (SDC) in some localities of Cameroon, Pharmacophore. Vol.7(4): 192-200.
10. Chen Z, Yu L, Wang X, Gu Z, Beta T. 2016. Changes of phenolic profiles and antioxidant activity in canaryseed (Phalaris canariensis L.) during germination. Food Chem. 194: 608-618.
11. Ndolo VU, Beta T. 2014. Comparative studies on composition and distribution of phenolic acids in cereal grain botanical fractions. Cereal Chem. 91(5): 522-530.
12. Yu LL., Beta T. 2015. Identification and antioxidant properties of phenolic compounds during production of bread from purple wheat grains. Molecules. 20(9): 15525-15549.
13. Joppa KM, Vovora, Eklu K, Agbonon A, Aklikokou K, Gbeassor M. 2008. Effet de Morinda Lucida Benth (Rubiaceae) et de NewbouldiaLeavis p. Beau v. (Bignoniaceae) sur la falciformation. Med. Trop. 68: 251-256.
14. Jaja SI, Kehinde MO, Gbenebitse S, Mojiminyi FBO, Ogungbemi AI. 2000. Effect of vitamin C on arterial blood pressure, irreversible sickled cells and osmotic fragility in sickle cell anemia subjects. Nig J Physio Sci. 16(1–2): 14–18.
15. Benzie I, Strain J. 1996. The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power: The FRAP Assay”. Analytical Biochemistry. 239: 70-76.
16. Jain A, Soni M, Deb L, Rout S, Gupta V, Krishna K. 2008. Antioxidant and hepatoprotective activity of ethanolic and aqueous extracts of Momordica dioica Roxb. leaves. J. Ethnopharmacol. 115(1): 61-66.
17. Yu L, Halley S, Perret J, Palma M, Wilson J, Qian M. 2002. Free radical scavenging properties of wheat extracts. Journal of Agricultural and Food Chemistry. 50: p.1619.
18. Maria NO, Antonio A, Filomena N, Riccardo R, Patrizia S, Massimo Z, Catello P,
19. Mena M, and Florinda F. 2016. Phenolic Composition and Antioxidant and Antiproliferative Activities of the Extracts of Twelve Common Bean (Phaseolus vulgaris L.) Endemic Ecotypes of Southern Italy before and after Cooking. Oxidative Medicine and Cellular Longevity. 2: 1-12.
20. Fan G, and Beta T. 2016. Proximate Composition, Phenolic Profiles and Antioxidant Capacity of Three Common Bean Varieties (Phaseolus vulgaris L.). J Food Chem Nanotechnol. 2(3):147-152.
21. Ramirez-Jimenez AK, Reynoso-Camacho R, Mendoza-Diaz S, Loarca-Pina G. 2014. Functional and technological potential of dehydrated Phaseolus vulgaris L. flours. Food Chem. 161: 254-260.
22. Joseph O, Phelomene M, Helene N, Valens H, Patrick OM, Thavarajah D and Thavarajah P. 2014. Phenolic Compound Profiles of Two Common Beans Consumed by Rwandans. American Journal of Plant Sciences. 5: 2943-2947.
23. Moody JO, Ojo OO, Omotade OO, Adeyemo AA, Olumese PE. 2003. Anti-sickling potential of a Nigerian herbal formula (ajawaron HF) and the major plant component (Cissus populnea L. CPK). Phytother Res. 17: 1173-1176.
24. Selva M, Carole T, John S, Xingqian Y,. Sophia JX. 2017. Ellagic acid in strawberry (Fragaria spp.): Biological, technological, stability, and human health aspects. Food Quality and Safety. 1(4): 227-252.
25. Kwuingoin I, Kotue TC, Kansci G, Marlyne JM and Fokou E. 2018. Assessment of mineral contents and antioxidant activities of some Bean seeds (Phaseolusvulgarus L) from west region cameroon. International journal of food and Nutritional Science. Vol 7: 57-63.
26. Javanmardia J, Stushnoffb C, Lockeb E, Vivancob JM. 2003. Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chemistry. 83: 547–550.
27. Vassale C, Massini S, Carpeggiani C, Abbale A, Boni C, Zucchelli G. 2004. In vivo total antioxydant capacity: comparison of two differents analytical methods. Clinical Chemistry and Laboratory Medicine.42: 84-89.