Compositional analysis of genetically modified soybeans placed on Taiwan market


Compositional analysis of genetically modified soybeans placed on Taiwan market


Huan-Yu Lin1, Jen-Tao Chen1, Mei-Li Chao1, Bo-Chou Chen1, Jo-Chi Wang1, Hsuen-Chun Liao1, Hui-Wen Chang1, Hsin-Tang Lin2 and Wen-Shen Chu1*

1Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu 300, Taiwan; 2Food and Drug Administration, Ministry of Health and Welfare, Taipei 115, Taiwan(Present: Graduate Institute of Food Safety, National Chung Hsing University, Taichung 402, Taiwan)


International Journal of Food and Nutrition Research

Soybean is an important protein source for consumers in Taiwan. Soybean production in Taiwan is not self-sufficient. Taiwan imports 2.5 million tons of soybeans annually. More than 90% of the imported soybeans are genetically modified (GM). To provide an objective assessment on safety of GM soybean and for post-market monitoring, we conducted a comparative assessment on key component compositions between imported GM soybean and local non-GM soybean from Taiwan. All the soybean samples were purchased from the local market to simulate the status of Taiwanese consumers in purchasing soybeans. The GM soybean samples were herbicide-tolerant soybeans. The content of the proximate, the amino acid composition, the fatty acid composition, vitamins, minerals, antinutritional factors, and isoflavones of soybean samples were analyzed. Most contents of the key components of the GM soybean had no significant difference with those of the non-GM soybean. However, the contents of ash, crude protein, amino acids, myristic acid, behenic acid, phosphorus, iron and phytic acid were significantly lower in the GM soybean samples, and the contents of crude fat, margaric acid, and stearic acid were significantly higher in the GM soybean samples. But they were all within the range of reference values. A total of 314 pesticide residues in each of the samples were analyzed. Glyphosate residue was detected only in GM soybean samples, but it is well below the threshold prescribed by the government. In summary, the GM soybean samples purchased from Taiwan market were shown to be substantially equivalent to non-GM soybeans.


Keywords:GM soybean, key component, substantially equivalent

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How to cite this article:
Huan-Yu Lin, Jen-Tao Chen, Mei-Li Chao, Bo-Chou Chen, Jo-Chi Wang, Hsuen-Chun Liao, Hui-Wen Chang, Hsin-Tang Lin and Wen-Shen Chu. Compositional analysis of genetically modified soybeans placed on Taiwan market. International Journal of Food and Nutrition Research, 2019; 3:31. DOI:10.28933/ijfnr-2019-08-2205


References:

1. AOAC. Official methods of analysis of the Association of Official Analytic Chemists International,19th edn. AOAC International, Gaithersburg, Maryland. 2012.
2. AOCS. Official methods and recommended practices of the AOCS, 6th edn. Association of Oil Chemists Society, Urbana, Illinois. 2009.
3. Babujia LC, Silva AP, Biondo PBF, Garcia JC, Mandarino JMG, Visentainer JV. (2015) Chemical composition of grains from glyphosate-resistant soybean and its conventional parent under different edaphoclimatic conditions in Brazil. Acta Sci Agron 37:463-471. DOI: 10.4025/actasciagron.v37i4.25108
4. Balisteiro DM, Rombaldi CV, Genovese MI (2013) Protein, isoflavones, trypsin inhibitory and in vitro antioxidant capacities: comparison among conventionally and organically grown soybeans. Food Res Int 51:8-14. DOI: 10.1016/j.foodres.2012.11.015
5. Barnes S, Kirk M, Coward L. (1994) Isoflavones and their conjugates in soy foods: extraction conditions and analysis by HPLC-mass spectrometry. J Agric Food Chem 42: 2466-2474.
6. Bøhn T, Cuhra M, Traavik T, Sanden M, Fagan J, Primicerio R (2014) Compositional differences in soybeans on the market: Glyphosate accumulates in Roundup Ready GM soybeans. Food Chem 153: 207-215.
7. Chen JT, Wen CY, Chao ML, Chu WS (2005) Studies on DNA extraction and quantitation detection of genetically modified maize using reference plasmid. Taiwanese J Agric Chem Food Sci 43:139-148.
8. Clarke DB, Lloyd AS. (2004) Dietary exposure estimate of isoflavones from the 1998 UK Total Diet Study. Food Addit Contam 21:305–316. DOI: 10.1080/02652030410001668781
9. Clive J. (2014) Global Status of Commercialized GM/Biotech Crops: 2014. ISAAA Brief 49. ISAAA : Ithaca, New York.
10. Clive J. (2015) 20th Anniversary of the global commercialization of biotech crops (1996 to 2015) and biotech crop highlights in 2015. ISAAA, BRIEF 51. ISAAA : Ithaca, New York.
11. Clive J. (2016) Global Status of Commercialized Biotech/GM Crops: 2016. ISAAA, Brief 52. ISAAA : Ithaca, New York.
12. ISAAA (2017) Global Status of Commercialized Biotech/GM Crops: 2017. ISAAA, Brief 53. ISAAA : Ithaca, New York.
13. Codex Alimentarius (2009) Foods derived from modern biotechnology. Second Edition, World Health Organization, Food and Agriculture Organization of the United Nations, Rome, Italy.
14. Council of Agriculture (COA) (2017) Food Supply and Utilization Yearbook.
http://agrstat.coa.gov.tw/sdweb/public/book/Book.aspx Taiwan. Accessed 14 Aug 2019
15. EU pesticides database. Code number 0401070. http://ec.europa.eu/food/plant/pesticides/eu-pesticides-database/public/?event=pesticide.residue.CurrentMRL&language=EN&pestResidueId=120. Accessed 11 May 2018
16. FAO/WHO (2016) Report of the joint meeting on pesticide residues.
http://www.who.int/foodsafety/jmprsummary2016.pdf?ua=1. Accessed 11 May 2018
17. Gao Y, Shanga C, Saghai Maroof MA, Biyasheva RM, Grabaub EA et al. (2007) A modified colorimetric method for phytic acid analysis in soybean. Crop Sci 47:1797-1803. DOI: 10.2135/cropsci2007.03.0122
18. Hwang EY, Song Q, Jia G, Specht JE, Hyten DL et al. (2014) A genome-wide association study of seed protein and oil content in soybean. BMC Genomics 15:1-12. DOI: 10.1186/ 1471-2164 -15 -1
19. ILSI Crop Composition Database v6.0. http://www.cropcomposition.org/query/index.html. Accessed 11 May 2019
20. OECD. (2012) Revised consensus document on compositional considerations for new varietirs of soybean [Glycine max (L.) Merr.]: Key food and feed nutrients, anti-nutrients, toxicants and allergens. ENV/JM/MONO 24. Series on the safety of novel foods and feeds No. 25. Organisation for Economic Co-Operation and Development, Paris, France.
21. Paulson JK, Kurai T (2017) Japan proposes the revision of MRLs for 7 agricultural chemicals. Gain Report. Gain Report Number: JA7053.
22. Pinthong R, Macrae R, Dick J (1980) The development of a soya‐based yoghurt. Food Sci Tech 15:661-667. DOI: 10.1111/j.1365-2621.1980.tb00987.x
23. Simpson RJ, Neuberger MR, Liu TY (1976) Complete amino acid analysis of proteins from a single hydrolysate. J Biol Chem 251:1936-1940.
24. Taiwan Food and Drug Administration (TFDA) (2015) Food labeling for genetically modified food. https://www.mohw.gov.tw/cp-2645-20513-1.html. Accessed 14 Aug 2019
25. Taiwan Food and Drug Administration (TFDA) (2019a) Current approvals of genetically modified foods in Taiwan.
https://consumer.fda.gov.tw/Food/GmoInfoEn.aspx?nodeID=300. Accessed 14 Aug 2019
26. Taiwan Food and Drug Administration (TFDA) (2019b) Standards for Pesticide Residue Limits in Foods. https://consumer.fda.gov.tw/Law/PesticideList.aspx?nodeID=520&rand=1215511388 Accessed 14 Aug 2019
27. Venkatesh TV, Cook K, Liu B, Perez T, Willse A et al. (2015) Compositional differences between near-isogenic GM and conventional maize hybrids are associated with backcrossing practices in conventional breeding. Plant Biotech J 13:200-210. DOI: 10.1111/pbi.12248