ENVIRONMENTAL ENRICHMENT REDUCES CORTICAL EXCITABILITY IN RATS SUBMITTED TO STATUS EPILEPTICUS IN THE PILOCARPINE MODEL: AN EVALUATION FROM CORTICAL SPREADING DEPRESSION


Environmental Enrichment Reduces Cortical Excitability In Rats Submitted To Status Epilepticus In The Pilocarpine Model: An Evaluation From Cortical Spreading Depression


Roberto Carlos da Silva Bandeira1, Luís Gustavo Carvalho dos Santos2, Christian Barker Silva Santisteban2, Angela Amancio-dos-Santos3

1Federal University of Pernambuco – UFPE, Health Sciences Center, Department of Pharmaceutical Sciences, Recife – PE, Brazil; 2Federal University of Pernambuco – UFPE, Medical Sciences Center, Recife – PE, Brazil; 3Federal University of Pernambuco – UFPE, Biosciences Center, Department of Physiology and Pharmacology, Recife – PE, Brazil


Objective: This study verified if the exposure to environmental enrichment (EE) influences brain excitability, evaluated by cortical spreading depression (CSD), in rats submitted to status epilepticus (SE).

Methods: At 35 days of age, male Wistar rats were divided into two experimental groups: pilocarpine (PILO), which received a single dose of pilocarpine hydrochloride to induce SE, and saline (SAL), which were the group control. On the following day, half of the animals in each group were exposed to EE; while the other half was kept in the standard environment (S), considered control. At 67 days of age, the rats were anesthetized and submitted to CSD.

Results: Pilocarpine, isolated, reduced the speed of propagation and increased the duration and amplitude of CSD. EE, itself, reduced the speed of propagation and duration, but increased the amplitude of CSD. In the animals exposed to EE that undergone SE, the values of CSD parameters became closer to the control group.

Conclusion: Our results indicate that EE reduced the cortical excitability induced by SE, possibly by promoting antioxidant effects at the brain level, inducing neurogenesis and/or improving the excitatory/inhibitory balance of neurotransmitters.


Keywords: epilepsy. cortical spreading depression. pilocarpine.

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How to cite this article:
Roberto Carlos da Silva Bandeira, Luís Gustavo Carvalho dos Santos, Christian Barker Silva Santisteban, Angela Amancio-dos-Santos. Environmental Enrichment Reduces Cortical Excitability In Rats Submitted To Status Epilepticus In The Pilocarpine Model: An Evaluation From Cortical Spreading Depression. International Journal of Neuroscience Research, 2021; 5:13. DOI: 10.28933/ijnr-2021-07-2005


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