Erythropoietic and hepatocurative profile of Yoyo Bitters® – A pilot study

Erythropoietic and hepatocurative profile of Yoyo Bitters® – A pilot study

Innocent U. Okagu1., Chinelo C. Nkwocha1., Adaeze Obi1., Nkiruka C. Ugochukwu1., Chukwudi Odo1., Christian C. Chibuogwu2*., Joseph C. Ndefo3., Victor N. Ogugua1., Sabinus I. Ofoefule2. and Benjamin C. Ozumba4

1Department of Biochemistry, University of Nigeria, Nsukka, Enugu State, Nigeria. 2Institute for Drug-Herbal Medicine-Excipient Research and Development, University of Nigeria, Nsukka, Enugu State, Nigeria.3Department of Science Laboratory Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria.4Department of Obstetrics and Gynecology, College of Medicine, University of Nigeria, Enugu Campus.

International Journal of Traditional and Complementary Medicine

The present study evaluated the effects of a branded Nigerian polyherbal formulation, Yoyo® bitters on the haematological and biochemical status of hydrogen peroxide (H2O2)-intoxicated rats. Thirty (30) healthy male Wistar rats used in this study were divided into 6 groups of 5 rats each. Group 1 served as normal control and received 3 ml/kg body weight (b.w.). of distilled water only while group 2 served as experimental control and was intoxicated with H2O2 without treatment. Rats in groups 3-5 were H2O2-intoxicated and treated with 1, 2 and 3 ml/kg b.w. of Yoyo® bitters respectively while rats in group 6 were H2O2-intoxicated and treated 100 mg/kg. b.w. of silymarin. H2O2 was administered intraperitoneal on day 0 while drugs were orally administered from days 0 to 14. The rats were sacrificed on day 15 and blood samples collected were subjected to haematological and biochemical analyses. Intoxication with H2O2 significantly (p < 0.05) induced haematotoxicity, oxidative stress and hepatotoxicity when compared with control. Treatment of intoxicated rats with the formulation restored the haematological and biochemical status to normal, suggesting that Yoyo® bitters has erythropoietic and hepatocurative effects. However, future studies are required to confirm these effects in different in vivo models.

Keywords: Yoyo® bitters, hepatocurative, haematology, antioxidants, silymarin, acute toxicity

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Innocent U. Okagu, Chinelo C. Nkwocha, Adaeze Obi, Nkiruka C. Ugochukwu, Chukwudi Odo, Christian C. Chibuogwu., Joseph C. Ndefo, Victor N. Ogugua, Sabinus I. Ofoefule. and Benjamin C. Ozumba. Erythropoietic and hepatocurative profile of Yoyo Bitters® – A pilot study. International Journal of Traditional and Complementary Medicine 2020, 5:24.


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