Research Article of International Journal of Traditional and Complementary Medicine
Anti-inflammatory effect of Phattapitta Recipe in RAW 264.7 macrophages stimulated with lipopolysaccharide
U. Nanna1*, S. Sireertwong2, K. Jaijoy3
1Division of Pharmacology, Department of Preclinical Science, Faculty of Medicine, Thammasat University, Rungsit Campus, Klong Loung, Pathum Thani 12120, Thailand. 2Department of Pharmacology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. 3McCormick Faculty of Nursing, Payap University, Chiang Mai 50000, Thailand.
Pattapitta Recipe has been used for the treatment of cirrhosis in the Thai Traditional Medicine Clinic of Prapokklao Hospital. The liver functions of cirrhotic patients treated with Pattapitta Recipe were improved and recovered to normal function within 5 months. Furthermore, Pattapitta Recipe has been used for the treatment of alcoholic liver cirrhosis, hepatitis-B, hepatitis-C, and cirrhosis due to unidentified causes. However, little is known about the mechanisms underlying its anti-inflammatory activities. This study is mainly focused on the cell viability test of Phattapitta Recipe on macrophages cell line RAW 264.7 and investigation of an anti-inflammatory action of Phattapitta Recipe on lipopolysaccharide (LPS)-induced macrophages cell line, RAW 264.7. The results showed that Phattapitta Recipe had no cytotoxicity in RAW 264.7 cells. Phattapitta Recipe 250 ug/mL possessed anti-inflammatory effects by significantly decreased mRNA levels of interleukin-1β(IL-1β), tumor necrosis factor –α (TNF-α), interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in macrophages cell line RAW 264.7. Moreover, Phattapitta Recipe also significantly inhibited the production of TNF-α and IL-6 at the protein level. Although Phattapitta Recipe treated cells showed a decreased trend of nitric oxide levels, it was not statistically significant reduction. The results from this study will be beneficial for the development of Phattapitta Recipe as a potential treatment of anti-inflammatory diseases.
Keywords: Anti-inflammatory effect, TNF-α, IL-1β, iNOS, COX-2
How to cite this article:
U. Nanna, S. Sireertwong, K. Jaijoy.Anti-inflammatory effect of Phattapitta Recipe in RAW 264.7 macrophages stimulated with lipopolysaccharide.International Journal of Traditional and Complementary Medicine 2019, 4:16.
1. Buapool, D., Mongkol, N., Chantimal, J., Roytrakul, S., Srisook, E., Srisook, K., 2013. Molecular mechanism of anti-inflammatory activity of Pluchea indica leaves in macrophages RAW 264.7 and its action in animal models of inflammation. J. Ethnopharmacol. 4, 495-504.
2. Choi, H.J., Kang, O.H., Park, P.S., Chae, H.S., Oh, Y.C., Lee, Y.S., Choi, J.G., Lee, G.H., Kweon, O.H., Kwon, D.Y., 2007. Mume Fructus water extract inhibits pro-inflammatory mediators in lipopolysaccharide-stimulated macrophages. J. Med. Food. 10, 460–466.
3. Diaz, P., Jeong, S.C., Lee, S., Khoo, C., Koyyalamudi, S.R., 2012. Anti-oxidant and anti-inflammatory activities of selected medicinal plants and fungi containing phenolic and flavonoid compounds. J. Chin. Med. 7, 26.
4. Nolan, T., Hands, R.E., Bustin, S.A., 2006. Quantification of mRNA using real-time RT-PCR. Nat. Protocols. 1(3), 1559–1582.
5. Oh, Y.C., Cho, W.K., Im, G.Y., Jeong, Y.H., Hwang, Y.H., Liang, C., Ma, J.Y., 2012. Anti-inflammatory effect of Lycium Fruit water extract in lipopolysaccharide-stimulated RAW 264.7 macrophage cells. Int. Immunopharmacol. 13(2), 181–189.
6. Vichai, V., Kirtikara, K., 2006. Sulforhodamine B colorimetric assay for cytotoxicity screening. Nat. Protocols. 1(3), 1112–1116.
7. Wang, J.X., Hou, L.F., Yang, Y., Tang, W., Li, Y., Zuo, J.P., 2009. SM905, an artemisinin derivative, inhibited NO and pro-inflammatory cytokine production by suppressing MAPK and NF-[kappa]B pathways in RAW 264.7 macrophages. Acta. Pharmacol. Sin. 30(10), 1428-1435.