Phytochemicals extracted from Cola nitida leaf possess antimalarial effects and improve derangements in haematological indices of Plasmodium berghei- infected mice.

Phytochemicals extracted from Cola nitida leaf possess antimalarial effects and improve derangements in haematological indices of Plasmodium berghei- infected mice.

Zailani, A. H., Iliyas, M. B., Benjamin, L., Ibrahim, B. A., Ubah, B., *Lamiya, A.

Department of Biochemistry, Modibbo Adama University of Technology, Yola, Adamawa State, Nigeria

International Journal of Traditional and Complementary Medicine

Classical antimalarial drugs such as quinine and artemisinin are both plant derived suggesting that plants are a promising source of bioactive components that can help in combating the scourge of malaria. Cola nitida leaf has been reported to possess antimalarial activity and also contain pharmacologically active phytochemicals including alkaloids, flavonoids, tannins and phenolics. The effects of these phytochemicals in mice infected with Plasmodium berghei was evaluated in this study. For each phytochemical, 7 groups (A-G) of eight mice each were used. Groups A and B served as normal and infected controls respectively. Group C was treated with 20 mg/Kg body weight of chloroquine and served as the treated control. Groups D, E and F were administered 12.5, 25 and 50 mg/kg body weight of the different phytochemicals while group F was treated with 50 mg/Kg body weight of the phytochemicals only without parasite inoculation thus serving as extract control. Treatment commenced 72 hrs after inoculation and was done once orally for four consecutive days after which parasitaemia was evaluated. All the phytochemicals were found to exhibit antimalarial activity in a dose dependent manner. The mean survival time of all the experimental groups were also prolonged in a dose dependent manner compared to that of untreated control. Similarly, all the phytochemicals improved the altered haematological indices towards normal. These phytochemicals of Cola nitida exhibited significant antimalarial activities and thus can be further studied in the search for novel antimalarial drugs.

Keywords: Antimalarial; phytochemical; in vivo; Cola nitida; haematological indices.

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How to cite this article:
Zailani, A. H., Iliyas, M. B., Benjamin, L., Ibrahim, B. A., Ubah, B., Lamiya, A.Phytochemicals extracted from Cola nitida leaf possess antimalarial effects and improve derangements in haematological indices of Plasmodium berghei- infected mice.International Journal of Traditional and Complementary Medicine 2020, 5:31. DOI: 10.28933/ijtcm-2020-04-0905


1. Abah, A. E. and Temple, A. (2015). Prevalence of malaria among asymptomatic primary school children in angiama community, Bayesa state of Nigeria, Trop. med. and surge. 4:203.
2. Kunihya, I. Z., Samaila A. B., Pukuma M. S. and Qadeer M. A. (2016).Prevalence of malaria infection and malaria anaemia among children attending Fedral Medical Center Yola, Adamawa State of Nigeria.The Int. J. of eng. and sci. 5(7): 8-14.
3. Kweyamba, P. A., Zofou, D., Efange, N., As-sob, J-C.N., Kitau, J. and Nyindo, M.(2019).Invitro and in vivo studies on antimalarial activity of Commiphora Africana and Dichrostachys Cinerea Used by the Maasai in Arusha region, Tanzania. Malar. J. .18:119
4. Bassey, S.E. and Izah, S. C. (2017).Some determinant factors in malaria prevalence in Nigeria.J. of Mosquito Res. 7(7); 48-58.
5. Muluye1, A. B., Desta1, A. G., Abate, S. K. and Dano, G. T. (2019). Antimalarial activity of the root extract of Euphorbia abyssinica(Euphorbiaceae) against Plasmodium berghei infection in mice. Malar. J. 18:261.
6. Rout, S. and Mahapatra, R. K. (2019) Plasmodium falciparum: multidrug resistance,Chemical boil. & drug. des., 93(5):737–759.
7. Ounjaijean, S., Kotepui, M. and Somsak, V. (2019).Antimalarial Activity of Tinospora Baenziger Against Plasmodium berghei-Infected Mice. J. of trop. Med.. 2019;6.
8. Zailani, H. A., Ibe, I.J. and Utor, O.J. (2016). Effects of aqueous leaf extract of Cola nitida on Parasitaemia, In vitro antioxidant and biochemical parameters in Plasmodium berghei infected Mice. J. of Health and pharmacol. 4(3):21-28.
9. Eromosele, O. J. and Kehinde, O. M.(2018).Phytochemical Study of Underutilized Leaves of Cola acuminata and Cola nitida. Am. Res. J. of Biosci. 4(I1):pp: 1-7.
10. Harborne, J. B. (1973). Phytochemical methods. Chapman and Hall ltd., London; 46-188
11. Yahaya, M. M. (2015). Isolation and Purification of Flavonoids from the Leaves of locally produced Carica papaya.Int. J. of sci. and technol. Res.4 (12): 282- 283.
12. Velioglu, Y. S., Mazza, G., Gao, L. and Oomah, B. D. (1998). antioxidant activity and total phenolics in selected fruits, vegetables and grain products. J. Agric. Food Chem; 46(10): 4113-7.
13. Marka A. O. S. and Goodchild, A. V., (1996).Quantification of tannins. A laboratory manual; international center for Agricultural research in the dry areas (ICARDA), Allepo, Syria,: 25
14. Dikasso, D., Mekonnen, E. and Debella, A. (2006). In vivo antimalarial activity of hydroalcoholic extracts from Asparagus africanus Lam. in mice infected with Plasmodium berghei. Ethiop J Health Dev. 20:112e118.
15. Laryea, M. K. and Borquaye, L. S. (2019).Antimalarial Efficacy and Toxicological Assessment of Extracts of Some Ghanaian Medicinal Plants.J. of Parasitol. Res. .2019:9, Article ID 1630405,
16. Kaur, H. and Mukhtar, H. M., Singh, A. and Mahajan, A. (2018).Antiplasmodial medicinal plants: a literature review on efficacy, selectivity and phytochemistry of crude plant extracts,” J. of Biolog-ically Act. Prod. from nat. ; 8(5): 272– 294,
17. Gebrehiwot, S., Shumbahri, M., Eyado, A. and Yohannes, T. (2019).Phytochemical Screening and In vivo Antimalarial Activity of Two Traditionally Used Medicinal Plants of Afar Region, Ethiopia, against Plasmodium berghei in Swiss Albino Mice. J. of Parasitol. Res. : 8 Article ID 4519298.
18. Okello, D. and Kang, Y.(2019).Exploring antimalarial herbal plants across communities in Uganda Based on Electronic Data.Evidence-based complementary and alternative medicine.Pp 1-27. Article ID 3057180.
19. Chinwuba, P. Akah, P. A. and Iiodigwe, E. E. (2015).In vivo antiplasmodial activity of the etha-nol stem extract and fractions of Citrus sinensis in mice,Merit Res. J. of Med. and Med. Sci. 3(4):140–146.
20. Freundlich, J. S., Anderson, J. W., Sarantakis, D. (2005). Synthesis, biological activity, and X-ray crystal structural analysis of diaryl ether inhibitors of malarial enoyl acyl carrier protein reductase. Part 1: 4′-substituted triclosa derivatives. biorgan. & Med. Chem. Lett.15(23):5247–5252.
21. Builders, M.I, Wannang, N.N, Ajoku, G.A, Build-ers, P.F, Orisadipe, A, Aguiyi, J.C. (2010).Evaluation of antimalarial potential of Vernonia ambigua Kitschy and Peyr (Asteraceae).Int. J. of Pharmacol. 18(11):1-9.
22. Meraiyebu, A., Akintayo, C.O. and Nenchi, D.Y. (2012).Evaluation of PCV and Hemoglobin Variations among Malaria Positive and Malaria Negative Patients, At the ECWA Community Health Centre Bukuru, Jos, Nigeria.IOSR. J. of Pharm.2: 65-69.
23. Francis, U., Isaac, Z., Yakubu, A., Enosakhare, A., Felix, E. (2014). Haematological Parameters of Malaria Infected Patients in the University of Calabar Teaching Hospital, Calabar, Nigeria. J. of Haematol. and Thromboembolic dis. 2: 171.
24. Bruce-Chwatt, L.J. (1982). Qinghaosu: a new antimalarial. British Medical Journal (Clinical Research Ed.) 284, 767–768 PATH Malaria Vaccine Initiative (MVI). p. 2.
25. Ahktar, K. P., Saleem, M. Y., Asghar, M., Alis.,Sarwar, N. and Elahi M. T. (2012). Resistance of Solanum species to Phytophthora Infestans evaluated in the detached leaf and whole plant assays; Pak. J. of bot., 44(3): 1141- 1146
26. Chandra, H. and Chandra, S. (2013). Role of hematological parameters as an indication of acute malarial infection in Uttrakhand state of India; Mediterr. J. of Haematol. and Infect. dis. DOI: 10.4084/MJHID.2013.009. PMC3552839.
27. Ifeanyichukwu MO, Esan A. (2014). Evaluation of blood cells and platelets in Plasmodium falciparum malaria infected individuals. Int. J. Hematol. Disord. 1:49–54.