A new release nanosystem mucoadhesive gel of Brazilian Red Propolis-containing chitosan: in vitro Citoxicity and Antimicrobial test. In vivo ligature induced periodontitis model- propolis treatment in rats

A new release nanosystem mucoadhesive gel of Brazilian Red Propolis-containing chitosan: in vitro Citoxicity and  Antimicrobial test. In vivo ligature induced periodontitis model- propolis treatment in rats

Cinthia Figueiredo Oliveira¹, Tatiana Gomes Ribeiro²,Flávio Henrique de Abreu Rosa¹ ,Adélia Cristina Souza Araújo¹,Rafael Tomaz Gomes¹,Rachel Oliveira Castilho²,André Augusto Gomes Faraco²,Mirian Tereza Paz Lopes³,Vagner Rodrigues Santos¹

¹Department of Oral Clinical, Pathology and Surgery, School of Dentistry, Universidade Federal de Minas Gerais; ²Department of Pharmaceutical Industry, School of Pharmacy, Universidade Federal de Minas Gerais; ³Laboratory of Pharmacology, Institut of Biological Sciences, Universidade Federal de Minas Gerais.

Red Propolis has been shown to be a potent antimicrobial, antinflammatory, antitumoral and antioxidant, however, is less studied against microorganisms and periodontal disease. This study aimed to investigate the antimicrobial, citoxicity properties and control of a induced periodontal pocket in rats of a red propolis mucoadhesive gel -containing chitosan. For this, we used two gels with concentrations of 5% (RPG5%) and 10% (RPG10%) of propolis and were compared with chitosan gel base 5% (CHG), propolis extract 5% (FRP5%) and chlorhexidine 0.12% (CHX0,12%). The products were tested against S. mutans, S. salivarius, S. sanguinis, L. casei, A. actinomycetemcomitans, E. faecalis, P. gingivalis, F. nucleatum, and C. albicans ATCC  standards. The Minimum Inhibitory Concentration, Concentration Minimal Bactericidal and agar diffusion tests were performed according to CLSI standards. Ligature induced periodontitis model were used and the gel product were applied in test and control rats group. The results showed that all microorganisms were sensitive to the gel RPG5% and RPG10%. There was a reduction in insertion loss and alveolar resorption in the animals treated with propolis gel. Chitosan appears to have not affected the antimicrobial activity of propolis and there seems to have been a synergism between the products. On the other hand, new tests and clinical trials studies should be performed to confirm these parameters.

Keywords: Brazilian red propolis, antimicrobial activity, mucoadhesive gel, chitosan, citotoxicity, induced periodontitis

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Cinthia Figueiredo Oliveira, Tatiana Gomes Ribeiro,Flávio Henrique de Abreu Rosa,Adélia Cristina Souza Araújo,Rafael Tomaz Gomes,Rachel Oliveira Castilho,André Augusto Gomes Faraco,Mirian Tereza Paz Lopes,Vagner Rodrigues Santos. A new release nanosystem mucoadhesive gel of Brazilian Red Propolis-containing chitosan: in vitro Citoxicity and Antimicrobial test. In vivo ligature induced periodontitis model- propolis treatment in rats..International Journal of Traditional and Complementary Medicine 2022, 7:40. DOI: 10.28933/ijtcm-2022-01-2805

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