Determination of Microbiological quality and detection of Thermotolerant fecal E. coli in ready to use water from Navsari city of South Gujarat

Determination of Microbiological quality and detection of Thermotolerant fecal E. coli in ready to use water from Navsari city of South Gujarat

A. P. Suthar, R. Kumar, C. V. Savalia, N. K. Patel and R. K. Patel
Department of Veterinary Public Health and Epidemiology, College of Veterinary science & Animal Husbandry, Navsari Agricultural University, Navsari-396 450, Gujarat. India

Fecal bacterial indicator analyses have been widely used for monitoring the water quality. The present study was designed to determine the density of Coliform and  Thermotolerant fecal E. coli from ready to use water samples collected  for a five month(from november-2016 to March-2017) period of monitoring from in and around Navsari city. A total of 73 samples were collected from different places and processed under standard bacteriological techniques. The EMB Agar was used as selective medium for isolation. The presumptive isolates of thermotolerant fecal E. coli were identified by Indole and Mac Conkey broth test (Acid & Gas) at 44°C  incubation and coliform were enumerated by MPN technique and E. coli were identified by metallic seen on EMB agar plate and biochemical test. Analysis of result revealed 11 isolates (15.06 %) (3 samples from Abrama village, 3 samples from fish market, 1 sample from PG hostel, 1 samples from Krishna hotel, 1 sample from Jay Ambe tea stall, 1 sample from Mahadev mandir and 1 sample from panipuri centre) of Thermotolerant fecal E. coli as well as E. coli  from 73  samples. All 11 isolates having presumptive number of coliform (MPN/100ml) ranging from 13-242 which may higher than the standard number which is below 10 according to BIS. The sensitivity pattern of Thermotolerant fecal E. coli with different antimicrobial agents was evaluated by D-test and showed cent percent resistant towards Amoxycilline followed in reducing levels by  18.18% each, of Cephalothine, Trimethoprime and Gatifloxacin and 9.09% each, of Tetracycline and Chloramphenicol. The pattern clearly indicated overall intermediate resistance to Cephalothine (36.36%), Gatifloxacin (36.36%). The isolates showed sensitivity towards 90.90% each, of Tetracycline and Chloramphenicol followed in reducing levels by Trimethoprime (81.81%) and 45.45% each, of Cephalothine and Gatifloxacin. The multiple antibiotic resistance MAR index was determined for each isolate which may raging from 0.16-0.83. The use of E. coli as the main bacterial indicator instead of other coliform bacteria has been proposed in water quality monitoring programs which tailors the microbiological quality of water.

Keywords: Antibiogram pattern, Thermotolerant, fecal, E. coli, MPN, MAR, Water, Navsari.

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A. P. Suthar, R. Kumar, C. V. Savalia, N. K. Patel and R. K. Patel.Determination of Microbiological quality and detection of Thermotolerant fecal E. coli in ready to use water from Navsari city of South Gujarat.International Research Journal of Public Health, 2018; 2:17. DOI:10.28933/irjph-2018-10-2804


1. Akram, M., Shahid, M. and Khan, A. U. (2007). Etiology and antibiotic resistance patterns of community-acquired urinary tract infections in J N M C Hospital Aligarh, India. Annals of Clinical Microbiology and Antimicrobials, 6(4): 1–10.
2. Amundson, D., Lindholm, C., Goyal, S. M. and Robinson, R. A. (1988). Microbial pollution of well water in southeastern Minnesota. Journal of Environmental Science & Health Part A, 23(5): 453-468.
3. Avşar, C. and Berber, İ. (2014). Plasmid profiling and antibiotics resistance of Escherichia coli strains isolated from Mytilus galloprovincialis and seawater. Journal of Coastal Life Medicine, 2(9): 689-693.
4. Bernhard, A. E. and Field, K. G. (2000). Identification of nonpoint sources of fecal pollution in coastal waters by using host-specific 16S ribosomal DNA genetic markers from fecal anaerobes. Applied and environmental microbiology, 66(4): 1587-1594.
5. Biyela, P. T., Lin, J. and Bezuidenhout, C. C. (2004). The role of aquatic ecosystems as reservoirs of antibiotic resistant bacteria and antibiotic resistance genes. Water Science and Technology, 50(1): 45-50.
6. Chandran, A., AA, M. H., Varghese, S. and Sheeja, K. M. (2008). Prevalence of multiple drug resistant Escherichia coli serotypes in a tropical estuary, India. Microbes and environments., 23(2): 153-158.
7. Edberg, S. C. L., Rice, E. W., Karlin, R. J. and Allen, M. J. (2000). Escherichia coli: the best biological drinking water indicator for public health protection. Journal of Applied Microbiology, 88(S1).
8. Faria, C., Vaz-Moreira, I., Serapicos, E., Nunes, O. C. and Manaia, C. M. (2009). Antibiotic resistance in coagulase negative staphylococci isolated from wastewater and drinking water. Science of the Total Environment, 407(12): 3876-3882.
9. Fawell, J. and Nieuwenhuijsen, M. J. (2003). Contaminants in drinking waterEnvironmental pollution and health. British medical bulletin, 68(1), 199-208.
10. Gaur, A., Ramteke, P. W., Pathak, S. P. and Bhattacherjee, J. W. (1992). Transferable antibiotic resistance among thermotolerant coliforms from rural drinking water in India. Epidemiology and infection, 109(1): 113.
11. Gomathinayagam, P., Davis, A. S., Hatha, A. A. and Lakshmanaperumalsamy, P. (1994). The risk assessment of faecal contamination by MAR indexing of Escherichia coli. International journal of hygiene and environmental medicine, 196(3): 279-283.
12. Krumperman, P. H. (1983). Multiple antibiotic resistance indexing of Escherichia coli to identify high-risk sources of fecal contamination of foods. Applied and Environmental Microbiology, 46(1): 165-170.
13. Kumar, H. S., Parvathi, A., Karunasagar, I. and Karunasagar, I. (2005). Prevalence and antibiotic rsistance of Escherichia coli in tropical seafood. World J. Microbiol. Biotechnol., 2: 619-623.
14. Kumar, R. and Singh, S.P. (2014). Pulse field gel electrophoresisprofiles of salmonella enterica serovar typhimurium isolates obtained from chickens sources. Indian Journal of Comparative Microbiology Immunology and Infectious, Diseases, 35: 64- 66.
15. Kumar, R., Dutta S. and Pal, D. (2017). Microbiological quality assessment and detection of fecal escherichia coli (coliform) in foods of animal origin sold in the open markets of kolkata city in India. Indian Journal of Comparative Microbiology Immunology and Infectious, Diseases, 38:22-25
16. Lim, L. C., Low, J. A. and Chan, K. M. (1999). Chryseobacterium meningosepticum (Flavobacterium meningosepticum) a report of five cases in a local hospital. Annals of the Academy of Medicine, Singapore, 28(6): 858-860.
17. Lin, J. and Biyela, P. T. (2005). Convergent acquisition of antibiotic resistance determinants amongst the Enterobacteriaceae isolates of the Mhlathuze River, KwaZulu-Natal (RSA). Water Sa., 31(2): 257-260.
18. Lobova, T. I., Barkhatov, Y. V., Ol’ga, V. S. and Popova, L. Y. (2008). Multiple antibiotic resistance of heterotrophic bacteria in the littoral zone of Lake Shira as an indicator of human impact on the ecosystem. Microbiological Research, 163(2): 152-160.
19. Łuczkiewicz, A., Jankowska, K., Fudala-Książek, S. and Olańczuk-Neyman, K. (2010). Antimicrobial resistance of fecal indicators in municipal wastewater treatment plant. Water research, 44(17): 5089-5097.
20. Martinez, J. L. (2009). Environmental pollution by antibiotics and by antibiotic resistance determinants. Environmental pollution, 157(11): 2893-2902.
21. McKeon, D. M., Calabrese, J. P. and Bissonnette, G. K. (1995). Antibiotic resistant gram-negative bacteria in rural groundwater supplies. Water Research, 29(8): 1902-1908.
22. Moore, J. E., Moore, P. J., Millar, B. C., Goldsmith, C. E., Loughrey, A., Rooney, P. J. and Rao, J. R. (2010). The presence of antibiotic resistant bacteria along the River Lagan. Agricultural water management, 98(1): 217-221.
23. Mulamattathil, S. G., Esterhuysen, H. A. and Pretorius, P. J. (2000). Antibiotic‐resistant Gram‐negative bacteria in a virtually closed water reticulation system. Journal of applied microbiology, 88(6): 930-937.
24. National Committee for Clinical Laboratory Standards (2014). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement., approved standard M100-S24. Wayne, PA.
25. Parveen, S., Murphree, R. L., Edmiston, L., Kaspar, C. W., Portier, K. M. and Tamplin, M. L. (1997). Association of multiple-antibiotic-resistance profiles with point and nonpoint sources of Escherichia coli in Apalachicola Bay. Applied and Environmental Microbiology, 63(7): 2607-2612.
26. Rompré, A., Servais, P., Baudart, J., De-Roubin, M. R. and Laurent, P. (2002). Detection and enumeration of coliforms in drinking water: current methods and emerging approaches. Journal of microbiological methods, 49(1): 31-54.
27. Scott, T. M., Rose, J. B., Jenkins, T. M., Farrah, S. R. and Lukasik, J. (2002). Microbial source tracking: current methodology and future directions. Applied and environmental microbiology, 68(12): 5796-5803.
28. Tallon, P., Magajna, B., Lofranco, C. and Leung, K. T. (2005). Microbial indicators of faecal contamination in water: a current perspective. Water, air, and soil pollution, 166(1-4): 139-166.
29. Toranzos, G. A., McFeters, G. A., Borrego, J. J. and Savill, M. (2002). Detection of microorganisms in environmental freshwaters and drinking waters. In Manual of Environmental Microbiology, Third Edition American Society of Microbiology, pp. 249-264).
30. Vaseeharan, B., Ramasamy, P., Murugan, T. and Chen, J. C. (2005). In vitro susceptibility of antibiotics against Vibrio spp. and Aeromonas spp. isolated from Penaeus monodon hatcheries and ponds. International journal of antimicrobial agents, 26(4): 285-291.
31. Völker, S., Schreiber, C. and Kistemann, T. (2010). Drinking water quality in household supply infrastructure A survey of the current situation in Germany. International journal of hygiene and environmental health, 213(3): 204-209.
32. Watkinson, A. J., Micalizzi, G. B., Bates, J. B. and Costanzo, S. D. (2007). Novel method for rapid assessment of antibiotic resistance in Escherichia coli isolates from environmental waters by use of a modified chromogenic agar. Appl. Environ. Microbiol., 73: 2224–2229.
33. World Health Organization. (2004). Guidelines for drinking-water quality (Vol. 1). World Health Organization.
34. Wose Kinge, C. and Mbewe, M. (2010). Characterisation of Shigella species isolated from river catchments in the North West province of South Africa. South African Journal of Science, 106(11-12): 1-4.
35. Zhang, Y., Marrs, C. F., Simon, C. and Xi, C. (2009). Wastewater treatment contributes to selective increase of antibiotic resistance among Acinetobacter spp. Science of the Total Environment, 407(12): 3702-3706.

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