Determination of Microbiological quality and detection of Thermotolerant fecal E. coli in ready to use water from Navsari city of South Gujarat

Determination of Microbiological quality and detection of Thermotolerant fecal E. coli in ready to use water from Navsari city of South Gujarat

A. P. Suthar, R. Kumar, C. V. Savalia, N. K. Patel and R. K. Patel
Department of Veterinary Public Health and Epidemiology, College of Veterinary science & Animal Husbandry, Navsari Agricultural University, Navsari-396 450, Gujarat. India

Fecal bacterial indicator analyses have been widely used for monitoring the water quality. The present study was designed to determine the density of Coliform and  Thermotolerant fecal E. coli from ready to use water samples collected  for a five month(from november-2016 to March-2017) period of monitoring from in and around Navsari city. A total of 73 samples were collected from different places and processed under standard bacteriological techniques. The EMB Agar was used as selective medium for isolation. The presumptive isolates of thermotolerant fecal E. coli were identified by Indole and Mac Conkey broth test (Acid & Gas) at 44°C  incubation and coliform were enumerated by MPN technique and E. coli were identified by metallic seen on EMB agar plate and biochemical test. Analysis of result revealed 11 isolates (15.06 %) (3 samples from Abrama village, 3 samples from fish market, 1 sample from PG hostel, 1 samples from Krishna hotel, 1 sample from Jay Ambe tea stall, 1 sample from Mahadev mandir and 1 sample from panipuri centre) of Thermotolerant fecal E. coli as well as E. coli  from 73  samples. All 11 isolates having presumptive number of coliform (MPN/100ml) ranging from 13-242 which may higher than the standard number which is below 10 according to BIS. The sensitivity pattern of Thermotolerant fecal E. coli with different antimicrobial agents was evaluated by D-test and showed cent percent resistant towards Amoxycilline followed in reducing levels by  18.18% each, of Cephalothine, Trimethoprime and Gatifloxacin and 9.09% each, of Tetracycline and Chloramphenicol. The pattern clearly indicated overall intermediate resistance to Cephalothine (36.36%), Gatifloxacin (36.36%). The isolates showed sensitivity towards 90.90% each, of Tetracycline and Chloramphenicol followed in reducing levels by Trimethoprime (81.81%) and 45.45% each, of Cephalothine and Gatifloxacin. The multiple antibiotic resistance MAR index was determined for each isolate which may raging from 0.16-0.83. The use of E. coli as the main bacterial indicator instead of other coliform bacteria has been proposed in water quality monitoring programs which tailors the microbiological quality of water.

Keywords: Antibiogram pattern, Thermotolerant, fecal, E. coli, MPN, MAR, Water, Navsari.

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A. P. Suthar, R. Kumar, C. V. Savalia, N. K. Patel and R. K. Patel.Determination of Microbiological quality and detection of Thermotolerant fecal E. coli in ready to use water from Navsari city of South Gujarat.International Research Journal of Public Health, 2018; 2:17. DOI:10.28933/irjph-2018-10-2804


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