Research Article of Journal of Herbal Medicine Research
Anti-bacterial Activity of Aqueous and Methanolic Root Extracts of Medicinal Plant Heteropogon contortus
Ruvani Gamagea, Buddhika Hasanthia,*, Rajith Silvab, Daya Ratnasooriyab, Ranjith Pathiranab, Sujeewa Hettihewac,
a National Science Foundation, Maitland Place, Colombo 7, Sri Lanka; b Department of Basic Sciences, Faculty of Allied Health Sciences, General Sir John Kotelawala Defence University, Werahera, Sri Lanka; cDepartment of Pharmacy, Faculty of Medicine, University of Ruhuna, Karapitiya, Sri Lanka
Synthetic drugs available for the treatment of chronic and infectious diseases are very expensive and some of them have become less effective due to the emergence of antibiotic resistance. Researchers are now focused on natural sources including plants and plant parts for the development of novel therapeutic agents. Therefore, this study evaluated in vitro anti-bacterial activity and phytochemical composition of aqueous and methanolic root extracts of Sri Lankan medicinal plant Heteropogon contortus against clinically significant pathogens, Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923). Agar well diffusion method and standard chemical tests were performed to evaluate anti-bacterial effect and phytochemical profile of the root extracts, respectively. The results revealed that each crude root extract exhibited anti-bacterial activity against both E. coli and S. aureus. The largest zones of inhibition against both E. coli and S. aureus were detected from the methanolic root extract at a concentration of 200 mg/mL and this is a novel finding. Phytochemical analysis of roots revealed the presence of bioactive constituents that account for the anti-bacterial potential, such as alkaloids, phenols and flavonoids. Thereby, root of H. contortus is a reliable source to develop potent, cheap and natural anti-bacterial agents. Future research focusing on high concentrations of different root extracts is recommended to further understand the anti-bacterial capability of H. contortus.
Keywords: Heteropogon contortus, medicinal plant, root, anti-bacterial activity
How to cite this article:
Ruvani Gamagea, Buddhika Hasanthia,*, Rajith Silvab, Daya Ratnasooriyab, Ranjith Pathiranab, Sujeewa Hettihewac. Anti-bacterial Activity of Aqueous and Methanolic Root Extracts of Medicinal Plant Heteropogon contortus. Journal of Herbal Medicine Research, 2019, 4:30.
1. Mujeeb F., Bajpai B and Pathak N. Phytochemical Evaluation, Antimicrobial Activity, and Determination of Bioactive Components from Leaves of Aegle marmelos. Biomed research International. 2014
2. Vines G. Herbal harvests with a future: towards sustainable sources for medicinal plants, 2004, Plant life International, http://www.plantlife.org.uk/ 20 February 2018.
3. Mothanaa RAA and Lindequistb U.Antimicrobial activity of some medicinal plants of the island Soqotra. Journal of Ethnopharmacology 2005; 96:177–181
4. Padalia H and Chanda S.Antimicrobial Efficacy of Different Solvent Extracts of Tagetes erecta L. Flower, Alone and in Combination with Antibiotics. Applied Microbiology: Open Acess . 2015; 1(1).
5. Ayurvedic medicinal plants of Sri Lanka http://www.instituteofayurveda.org/plants/plants_detail.php?i=1030&s=Family_name 20 February 2018.
6. Jayaweera DMA. Medicinal plants (indigenous and exotic) used in Ceylon, Part III, The National Science Council of Sri Lanka, Colombo, 1981, 39.
7. Ghante MH., Bhusari KP., and Duragkar NJ. Bronchorelaxent and anti-inflammatory effect of Heteropogon contortus (L.) Beauv. methanolic extract. International Journal of Pharmaceutical Technology and Research. 2013; 5: 99–104.
8. Kaur N., and Gupta RC. Lupeol validation and quantification in Heteropogon contortus (L.) Beauv. (spear grass) through high-performance thin-layer chromatography. Asian Journal of Pharmaceutical and Clinical Research. 2017; 12: 392–395.
9. Omojate Godstime C., Enwa Felix O., Jewo Augustina O. and Eze Christopher O. Mechanisms of antimicrobial actions of phytochemicals against enteric pathogens–a review. Journal of Pharmaceutical, Chemical and Biological Sciences. 2014; 2(2): 77-85.
10. Balouiri M., Sadiki M. and Ibnsouda S.K. Methods for in vitro evaluating antimicrobial activity: a review. Journal of Pharmaceutical Analysis. 2016; 6(2): 71-79.
11. Magaldi S., Mata-Essayag S., De Capriles C.H., Perez C., Colella M.T., Olaizola C. and Ontiveros Y. Well diffusion for antifungal susceptibility testing. International Journal of Infectious Diseases. 2004; 8(1): 39-45.
12. Valgas C., Souza S.M.D., Smânia E.F. and Smânia Jr A. Screening methods to determine antibacterial activity of natural products. Brazilian Journal of Microbiology. 2007; 38(2): 369-380.
13. Rasheed M.U., Thajuddin N., Ahamed P., Teklemariam Z. and Jamil K. Antimicrobial drug resistance in strains of Escherichia coli isolated from food sources. Revista do Instituto de Medicina Tropical de São Paulo. 2014; 56(4): 341-346.
14. Shakya P., Shrestha D., Maharjan E., Sharma V.K. and Paudyal R. ESBL production among E. coli and Klebsiella spp. causing urinary tract infection: a hospital based study. The Open Microbiology Journal. 2017; 11: 23-30.
15. Chen C.J. and Huang Y.C. New epidemiology of Staphylococcus aureus infection in Asia. Clinical Microbiology and Infection. 2014; 20(7): 605-623.
16. Balouiri M., Sadiki M. and Ibnsouda S.K. Methods for in vitro evaluating antimicrobial activity: a review. Journal of Pharmaceutical Analysis. 2016; 6(2): 71-79.
17. Amber, R., Adnan, M., Tariq, A., Khan, S.N., Mussarat, S., Hashem, A., Al-huqail, A.A., Al-Arjani, A.B.F. and Abd_Allah, E.F. Antibacterial activity of selected medicinal plants of northwest Pakistan traditionally used against mastitis in livestock. Saudi Journal of Biological Sciences. 2017; 25(2018): 154-161.
18. Mussarat, S., AbdEl-Salam, N.M., Tariq, A., Wazir, S.M., et al., 2014a. Use of ethnomedicinal plants by the people living around Indus River. Evid. Based Compl. Alter. Med., 1–14.
19. Mussarat, S., Amber, R., Tariq, A., Adnan, M., et al., 2014b. Ethnopharmacological assessment of medicinal plants used against livestock infections by the people living around Indus River. BioMed. Res. Int., 1–14.
20. Cushnie T.T., Cushnie B. and Lamb A.J. Alkaloids: an overview of their antibacterial, antibiotic-enhancing and antivirulence activities. International Journal of Antimicrobial Agents. 2014; 44(5); 377-386.
21. Maddox C.E., Laur L.M. and Tian L. Antibacterial activity of phenolic compounds against the phytopathogen Xylella fastidiosa. Current Microbiology. 2010; 60(1): 53-58.
22. Cushnie T.T. and Lamb A.J. Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents. 2005; 26(5): 343-356.
23. Cushnie, T.T., Cushnie, B. and Lamb, A.J., 2014. Alkaloids: an overview of their antibacterial, antibiotic-enhancing and antivirulence activities. International Journal of Antimicrobial Agents, 44(5), pp.377-386.
24. Spirig, T., Weiner, E.M. and Clubb, R.T., 2011. Sortase enzymes in Gram‐positive bacteria. Molecular microbiology, 82(5), pp.1044-1059.
25. Al-Bayati F.A. and Al-Mola H.F. Antibacterial and antifungal activities of different parts of Tribulus terrestris L. growing in Iraq. Journal of Zhejiang University-Science B. 2008; 9(2): 154-159.
26. Pereira C.A., Costa A.C.B.P., Liporoni P.C.S., Rego M.A. and Jorge A.O.C. Antibacterial activity of Baccharis dracunculifolia in planktonic cultures and biofilms of Streptococcus mutans. Journal of Infection and Public Health. 2016; 9(3): 324-330.
27. Orhan, D.D., Özçelik, B., Özgen, S. and Ergun, F., 2010. Antibacterial, antifungal, and antiviral activities of some flavonoids. Microbiological Research, 165(6), pp.496-504.
28. Cowan, M.M. Plant products as antimicrobial agents. Clinical Microbiology Reviews. 1999; 12(4): 564-582.
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