Research Article of Journal of Herbal Medicine Research
Anti-bacterial Activity of Aqueous and Methanolic Root Extracts of Medicinal Plant Heteropogon contortus
Ruvani Gamagea, Buddhika Hasanthia,*, Rajith Silvab, Daya Ratnasooriyab, Ranjith Pathiranab, Sujeewa Hettihewac,
a National Science Foundation, Maitland Place, Colombo 7, Sri Lanka; b Department of Basic Sciences, Faculty of Allied Health Sciences, General Sir John Kotelawala Defence University, Werahera, Sri Lanka; cDepartment of Pharmacy, Faculty of Medicine, University of Ruhuna, Karapitiya, Sri Lanka
Synthetic drugs available for the treatment of chronic and infectious diseases are very expensive and some of them have become less effective due to the emergence of antibiotic resistance. Researchers are now focused on natural sources including plants and plant parts for the development of novel therapeutic agents. Therefore, this study evaluated in vitro anti-bacterial activity and phytochemical composition of aqueous and methanolic root extracts of Sri Lankan medicinal plant Heteropogon contortus against clinically significant pathogens, Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923). Agar well diffusion method and standard chemical tests were performed to evaluate anti-bacterial effect and phytochemical profile of the root extracts, respectively. The results revealed that each crude root extract exhibited anti-bacterial activity against both E. coli and S. aureus. The largest zones of inhibition against both E. coli and S. aureus were detected from the methanolic root extract at a concentration of 200 mg/mL and this is a novel finding. Phytochemical analysis of roots revealed the presence of bioactive constituents that account for the anti-bacterial potential, such as alkaloids, phenols and flavonoids. Thereby, root of H. contortus is a reliable source to develop potent, cheap and natural anti-bacterial agents. Future research focusing on high concentrations of different root extracts is recommended to further understand the anti-bacterial capability of H. contortus.
Keywords: Heteropogon contortus, medicinal plant, root, anti-bacterial activity
How to cite this article:
Ruvani Gamagea, Buddhika Hasanthia,*, Rajith Silvab, Daya Ratnasooriyab, Ranjith Pathiranab, Sujeewa Hettihewac. Anti-bacterial Activity of Aqueous and Methanolic Root Extracts of Medicinal Plant Heteropogon contortus. Journal of Herbal Medicine Research, 2019, 4:30.
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