Evaluation of antimicrobial efficacy of ethanolic extract of Panax ginseng against Periodontal pathogens: An invitro study


Evaluation of antimicrobial efficacy of ethanolic extract of Panax ginseng against Periodontal pathogens: An invitro study


1Amulya. K.S., 2Jayasheela M., 3Gayathri G., 4Mehta D.S.

1Post graduate student, Department of Periodontics, Bapuji dental college and hospital, Davangere, Karnataka, India; 2Professor, Department of Periodontics, Bapuji dental college and hospital, Davangere, Karnataka, India; 3Professor, Head of the department, Department of Periodontics, Bapuji dental college and hospital, Davangere, Karnataka, India; 4Professor, Department of Periodontics, Bapuji dental college and hospital, Davangere, Karnataka, India


The present experimental study was carried out with the aim of evaluating the antibacterial activity of ethanolic extract of Panax ginseng on the most common periodontal pathogens, Porphyromonas gingivalis (Pg) Prevotella intermedia (Pi), Fusobacterium nucleatum (Fn) and Aggregatibacter actinomycetemcomitans (Aa). Ethanolic extract of Panax ginseng was prepared using maceration technique. Thus obtained extract was tested for its antimicrobial efficacy against 4 periodontal pathogens by means of evaluating the Minimum inhibitory concentration (MIC), Minimum bactericidal concentration (MBC) and time kill curve analysis. MIC was carried out by using serial dilution method. MBC was assessed by incubating the cultures of microorganisms with the same drug concentrations as MIC. Time kill curve (TKC) was analysed by incubating the organisms in anaerobic chamber, to evaluate the timely declination of growth rate. The results indicated that the ethanolic extract of Panax ginseng was both bacteriostatic and bactericidal for Pg and Pi at 0.8 µg/ml and for Fn at 50 µg/ml. But for Aa, it was only bacteriostatic at 25 µg/ml but not bactericidal in nature. Also, Pg showed no growth after 10 minutes, but the growth of Pi, Fn and Aa was not inhibited even after incubation for 2 hours. From this in-vitro study we conclude that the ethanolic extract of panax ginseng showed antimicrobial efficacy against all the test microorganisms, such as Pg, Fn, Pi and Aa. Thus these results can be used to continue with further invitro, invivo research and in day to day clinical treatment of chronic periodontitis.


Keywords: antimicrobial efficacy, ethanolic extract of Panax ginseng, Periodontal pathogens


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How to cite this article:
Amulya. K.S., Jayasheela M., Gayathri G., Mehta D.S. . Evaluation of antimicrobial efficacy of ethanolic extract of Panax ginseng against Periodontal pathogens: An invitro study. Journal of Herbal Medicine Research, 2020,5:39.


References:

1. Kalsi R, Vandana KL, Prakash S. Effect of local drug delivery in chronic periodontitis patients: A meta-analysis. J Indian Soc Periodontol. 2011: 15; 304 – 9.
2. Greenstein G. Nonsurgical periodontal therapy in 2000: a literature review. J Am Dent Assoc. 2000; 131:1; 580 – 92.
3. Cosyn J, Wyn I. A systematic review on the effects of the chlorhexidine chip when used as an adjunct to scaling and root planing in the treatment of chronic periodontitis. J Periodontol. 2006; 77: 257 – 64.
4. Hanes PJ, Purvis JP. Local anti‐infective therapy: pharmacological agents. A systematic review. Ann periodontol. 2003; 8: 79 – 98.
5. Yun TK. Brief introduction of Panax ginseng C.A. Meyer. J Korean Med Sci 2001;16:S3-S5.
6. Kim YJ, Zhang D, Yang DC. Biosynthesis and biotechnological production of ginsenosides. Biotechnol Adv 2015; 33:717-735.
7. Kim WY, Kim JM, Han SB, Lee SK, Kim ND, Park MK, Kim CK, Park JH. Steaming of ginseng at high temperature enhances biological activity. J Nat Prod 2000; 63:1702-1704.
8. Attele AS, Wu JA, Yuan CS. Ginseng pharmacology: multiple constituents and multiple actions. Biochem Pharmacol, 1999; 58, 1685–1693.
9. Scaglione F, Ferrara F, Dugnani S, Falchi M, Santoro G, Fraschini F. Immunomodulatory effects of two extracts of Panax ginseng C.A. Meyer. Drugs Exp Clin Res 1990; 16: 537-542.
10. Foerster et al. Time-kill curve analysis and pharmacodynamic modelling for in vitro evaluation of antimicrobials against Neisseria gonorrhoeae. BMC Microbiol. 2016; 16:216-21
11. Xue P, Yao Y, Yang XS, Feng J, Ren GX. Improved antimicrobial effect of ginseng extract by heat transformation. J Ginseng Res 2017; 41:180-187.
12. Wu H, Lee B, Yang L, Wang H, Givskov M, Molin S, Høiby N, Song Z. Effects of ginseng on Pseudomonas aeruginosa motility and biofilm formation. FEMS Immunol Med Microbiol. 2011; 62: 49 – 56.
13. Kang OJ. Antioxidant Activities of Various Solvent Extracts from Ginseng (Panax ginseng CA Meyer) Leaves. Prev Nut and Food Sci. 2011; 16: 321 – 7.
14. Jung CH, Seog HM, Choi IW, Park MW, Cho HY. Antioxidant properties of various solvent extracts from wild ginseng leaves. LWT-Food Sci Technol. 2006; 1;39: 266-74.
15. Sung WS, Lee DG. The combination effect of Korean red ginseng saponins with kanamycin and cefotaxime against methicillin-resistant Staphylococcus aureus. Biol Pharm Bull. 2008: 31: 1614 – 7.
16. Na S, Kim JH, Rhee YK, Oh SW. Enhancing the antimicrobial activity of ginseng against Bacillus cereus and Staphylococcus aureus by heat treatment. Food Sci Biotechnol. 2017; 27: 203 – 210.
17. Song Z, Kong KF, Wu H, Maricic N, Ramalingam B, Priestap H, Schneper L, Quirke JM, Høiby N, Mathee K. Panax ginseng has anti-infective activity against opportunistic pathogen Pseudomonas aeruginosa by inhibiting quorum sensing, a bacterial communication process critical for establishing infection. Phytomed. 2010; 17: 1040 – 6.
18. Park JS, Shin JA, Jung JS, Hyun JW, Van Le TK, Kim DH, Park EM, Kim HS. Anti-infl ammatory mechanism of compound K in activated microglia and its neuroprotective effect on experimental stroke in mice. J Pharmacol Exp Ther 2012; 341: 59 – 67.
19. Lee JH, Lee JS, Chung MS, Kim KH. In vitro anti-adhesive activity of an acidic polysaccharide from Panax ginseng on Porphyromonas gingivalis binding to erythrocytes. Planta medica. 2004; 70: 566 – 9.
20. Lee JH, Shim JS, Lee JS, Kim MK, Chung MS, Kim KH. Pectin like acidic polysaccharide from Panax ginseng with selective antiadhesive activity against pathogenic bacteria. Carbohydr Res 2006; 341:1154-1163.