Xanthigen® reduces lipid deposition and improves stress resistance in Caenorhabditis elegans

Xanthigen® reduces lipid deposition and improves stress resistance in Caenorhabditis elegans

María Dolores Jimenez1, Laura López-Ríos2*, Rubén Pérez-Machín2, Ricardo Chirino3, Barbara Davis4, Elena Talero1#, Virginia Motilva1#

1Department of Pharmacology, Faculty of Pharmacy, Universidad de Sevilla, Seville, Spain; 2Nektium Pharma SL, Las Palmas, Spain; 3Department of Biochemistry, Molecular Biology and Physiology, Faculty of Health Science, University of Las Palmas de Gran Canaria (ULPGC), Las Palmas, Spain; 4PLT Health Solutions, Inc., Morristown, New Jersey, USA

Xanthigen® is a nutraceutical combination of two well-known natural products, brown seaweed extract (rich in fucoxanthin) and pomegranate seed oil (rich in punicic acid), and it has been designed to use in weight management, in conjunction with a calorie restricted diet. In the nematode Caenorhabditis elegans Xanthigen® treatment caused a significant reduction in lipid deposition in wild-type N2 (WT-N2) animals but not in sirt-2.1-deficient strain, which raises the possibility that the prolipolytic or anti-lipogenic effect of Xanthigen® in these animals is mediated through Sirtuin 2.1 activation. This response has been well described for Xanthigen® in cell cultures and other animal models. In addition, Xanthigen® treatment conferred to both strains an increased resistance to thermal and oxidative stress, which opens the possibility that the effects of Xanthigen® are not mediated solely by Sirtuin 2.1 activation. We therefore explored whether Xanthigen® could activate diverse defence mechanisms such as DAF-16 activation, or GST induction in response to xenobiotics, by using the strains TJ356, CL2070 and CL2166, stably expressing Pdaf-16::GFP, Phsp-16.2::GFP and Pgst-4::GFP, respectively. Xanthigen® treatment provoked neither DAF-16 translocation to the nucleus nor increased expression of HSP16.2 and GST4, which opens the possibility that different mechanisms other than DAF-16 and those involved in xenobiotic responses, are activated by Xanthigen® and are capable of conferring to the nematode an increased resistance to thermal or oxidative stress.

Keywords: Xanthigen®, fucoxanthin, punicic acid, Caenorhabditis elegans, sirtuin 2.1, lipid deposition, thermal stress, oxidative stress

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María Dolores Jimenez, Laura López-Ríos, Rubén Pérez-Machín, Ricardo Chirino, Barbara Davis, Elena Talero, Virginia Motilva. Xanthigen® reduces lipid deposition and improves stress resistance in Caenorhabditis elegans. Journal of Herbal Medicine Research, 2020,5:41. DOI: 10.28933/jhmr-2019-1805


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