Research article of Journal of Plant and Environmental Research
Element content, growth and metabolic changes in Cu- and Cd- stressed Phaseolus vulgaris plants
Mahmoud E. Younis *, Shaimaa M. N. Tourky and Shaimaa E. A. Elsharkawy
Botany Department, Faculty of Science, University of Mansoura, Mansoura, Egypt
A large-scale pot experiment was accomplished for investigation of the varied effects of different concentrations of Cu and Cd on certain growth and metabolic attributes of roots and shoots of Phaseolus vulgaris plants, over a period of three weeks. Plants supplemented with Cu and Cd at the concentrations of 10-6 and 10-3 M, showed increased levels of Cu and Cd in both shoots and roots, above those levels in controls. However, Cu or Cd accumulation was lower in shoots than in roots. As compared with control levels, the low (10-6 M) concentration of Cu induced either a significant or an insignificant increase in growth parameters, photosynthetic pigments, PS II activity, glucose, proline and glycine contents in both roots and shoots. Otherwise, insignificant decreases in fructose, sucrose, polysaccharides, total saccharides, total soluble-N, protein –N, DNA and RNA contents, in the same test plant parts, were obtained. A reverse situation was however observed with the high concentration (10-3 M) of Cu as well as with the low and high concentrations (10-3 and 10-6 M) of Cd. In general, the observed adverse effects were more pronounced with Cd at (10-6 M) as compared with those maintained with Cu at the same concentration. Furthermore, the most detrimental adverse effects were apparent upon administration of the high (10-3 M) concentration of Cd. The prominence of the above mentioned changes in growth and metabolism to stress tolerance in common bean is discussed.
Keywords: Phaseolus vulgaris, Cu, Cd, growth parameters, photosynthetic components, carbohydrate and nitrogenous constituents and nucleic acids.
How to cite this article:
Mahmoud E. Younis, Shaimaa M. N. Tourky and Shaimaa E. A. Elsharkawy.Element content, growth and metabolic changes in Cu- and Cd- stressed Phaseolus vulgaris plants. Journal of Plant and Environmental Research, 2018,3:9. DOI:10.28933/jper-2018-07-2001
- Abd El-Fattah EA, Hashim EA, Fathy EA. Influence of cadmium on the growth and metabolic contents of Ambrosia maritima. EJSS. 2003; 43: 125-141.
- Bates LS, Waldren RP, Teare ID . Rapid determination of free proline for water stress studies. Plant Soil. 1973; 39: 205-207.
- Bayer EM, Bottrill AR, Walshaw J, Vigouroux M, Naldrett, Thomas CL. Arabidopsis cell wall proteome defined using multidimensional protein identification technology. Proteomics. 2006; 6: 301–311.
- Begum NA, Izumi N, Nishikori M, Nagaoka H, Shinkura R, Honjo T. Requirement of non-canonical activity of uracil DNA glycosylase for class switch recombination. J Biol Chem. 2007; 282: 731–742.
- Bibi M, Hussain M. Effect of copper and lead on photosynthesis and plant pigments in black gram [Vigna mungo (L.) Hepper]. Environ. Contam. Toxico. 2005; l74:1126-33.
- Dean RL, Miskiewiez E. Rates of electron transport in the thylakoid membranes of isolated, illuminated chloroplasts are enhanced in the presence of ammonium chloride. Biochem Mol Biol. 2003 ; 31:410-417.
- Deef HES. Copper treatment and their effects on growth, carbohydrates, minerals and essential oils contents of Rosmarinus officinalis World J Agric Sci. 2007 ; 3: 322-328.
- Demirevska-Kepova K, Simova-Stoilova L, Stoyanova Z, Holzer R, Feller U. Biochemical changes in barley plants after excessive supply of copper and manganese. Environ Exp Bot. 2004 ; 52: 253-266.
- Devi P. Principles and Methods of Plant Molecular Biology, Biochemistry and Genetics. Agrobios, India. 2002; 41: 57-59.
- Hamid N, Bukhari N, Jawaid F. Physiological responses of Phaseolus vulgaris to different lead concentrations. Pak J Bot. 2010 ; 42: 239-246.
- Hasaneen MNA, Younis ME, El-Bialy DMA. Plant growth, metabolism and adaptation in relation to stress conditions XXII. Further studies supporting nullification of harmful effects of salinity in lettuce plants by urea treatment. Plant Soil Environ. 2009 ; 54: 123-131.
- Hatata MM, Abdel-Aal EA. Oxidative stress and antioxidant defense mechanisms in response to cadmium treatments. American-Eurasian J Agric Environ Sci. 2008; 4: 655-669.
- Hegedus A, Erdei S, Horvath G. Comparative studies of H2O2 detoxifying enzymes in green and greening barley seedlings under cadmium stress. Plant sci. 2001; 160: 1085-1093.
- Jain M, Mathur G, Koul S, Sarin NB. Ameliorative effects of proline on salt stress induced lipid peroxidation in cell lines of groundnut (Arachis hypogea ). Plant Cell Rep. 2001; 20: 463-468.
- Kumar SV, Rajam MV. Metabolic engineering of carbohydrates for abiotic stress tolerance. In: Nandi, S. K., Palni, L. M. S. and Kumar, A. (eds.), Role of Plant tissue culture in biodiversity conservation and economic development. Gyanodaya Prakashan, Nainital, India., 2002 ; pp. 479-489.
- Kuriakose SV, Prasad MNV. Cadmium stress affects seed germination and seedling growth in Sorghum bicolor Moench by changing the activities of hydrolyzing enzymes. Plant Growth Regul. 2008;54:143-156.
- Küpper H, Küpper F, Spiller M. In situ detection of heavy metal substituted chlorophylls in water plants. Photosynth Res.1998; 58: 125-133.
- Madhavi A, Rao AP. Effect of cadmium on plant growth and uptake of nutrients by fodder sorghum, greengram and lucerne. J Res ANGRAU. 1999; 27:15-23.
- Manivasagaperumal R, Balamurugan S, Thiyagarajan G, Sekar J. Effect of zinc on germination, seedling growth and biochemical content of Cluster Bean (Cyamopsis tetragonoloba (L.) Taub). Curr Bot. 2011; 2: 11-15.
- Mediouni C, Benzari O, Tray B, Ghorbel M H, Jemal F. Cadmium and copper toxicity for tomato seedlings. Agronomy for Sustainable Development, Springer Verlag/EDP Sciences/INRA., 2006 ; 26: 227-232.
- Metzner H, Rau H, Senger H. Untersuchungen zur synchronisierbarkeit einzelner pigment-mangel mutanten von Chlorella. Planta. 1965 ; 65: 186–194.
- Mohanpuria P, Rana NK, Yadav SK. Cadmium induced oxidative stress influence on glutathione metabolic genes of Camellia sinensis (L.) O. Kuntze. Environ Toxicol. 2007; 22: 368-374.
- Motsara MR, Roy RN. Guide to laboratory establishment for plant nutrient analysis. Food and agricultural organisation of the United Nations FAO Fertilizer and Plant Nutrition Bulletin. Rome 2008.
- Muting D, Kaiser E. Spectrophotometric method of determining of alfa-amino-N in biological material by means of the ninhydrin reaction. Hoppe Seyler`s Zschr Physiol Chem. 1963 ; 332: 276-289 .
- Prasad MNV, Strzalka K. Physiology and biochemistry of metal toxicity and tolerance in plants. Kluwer Academic Publishers. Dordrecht, 2002. p.432.
- Sadasivam S, Manickam A. Biochemical methods. (2nd edition). New Age International (P) Limited, Publishers, New Delhi, 1996 . pp 179-186.
- Schat H, Sharma SS, Vooijs R. Heavy metal-induced accumulation of free proline in a metal-tolerant and a nontolerant ecotype of Silene vulgaris. Physiol Plant. 1997 ; 101: 477–482.
- Sheoran IS, Singal HR , Singh R. Effect of cadmium and nickel on photosynthesis and the enzymes of the photosynthetic carbon reduction cycle in pigeonpea (Cajanus cajan). Photosynth Res. 1990; 23: 345-351.
- Souguir D, Ferjani E, Ledoigt G, Gopupil P. Exposure of Vicia faba and Pisum sativum to copper induced genotoxicity. Protoplasma. 2008 ; 233: 203-207.
- Stewart GR, Lee AJ. The role of proline accumulation in halophytes. Planta. 1974 ; 120:279-289.
- Subbaiah CC, Sachs MM. Molecular and cellular adaptations of maize to flooding stress. Ann Bot. 2003; 91:119-127.
- Thayumanavan B, Sadasivam S. Physicochemical basis for the preferential uses of certain rice varieties. Plant Food Hum Nutr. 1984 ; 34:253-259.
- Trebst A. Measurements of Hill reactions and In Colowick, S. P., Kaplan, N. O. (ed.). Methods in Enzymology, 1972; 24:146-153. Academic press, New York.
- Van Handel E . Direct microdetermination of sucrose. Anal Biochem. 1968 ; 22:280-283.
- Waditee R, Bhuiyan M N H, Rai V, Aoki K, Tanaka Y, Hibino T, Suzuki, S, Takano J, Jagendorf A, Takabe, T, Takabe, T . Genes for direct methylation of glycine provide high levels of glycinebetaine and abiotic-stress tolerance in Synechococcus and Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America, 2005; 102:1318–1323.
- Wani P, Saghir Khan M, Zaidi A. Effect of metal tolerant plant growth promoting Bradyrhizobium sp. (vigna) on growth, symbiosis, seed yield and metal uptake by greengram plants. Chemosphere. 2007 ; 70: 36-45.
- Wisniewski L, Dickinson NM. Toxicity of copper to Quercus robur (English Oak) seedlings from a copper-rich soil. Environ Exp Bot. 2003; 50: 99-107.
- Yemm EW, Willis AJ. The respiration of barley plants. IX. The metabolism of roots during the assimilation of nitrogen. New Phytol. 1956; 55: 229-252.
- Younis ME, Hasaneen MNA, Tourky SMN. Plant growth, metabolism and adaptation in relation to stress conditions. XXIV. Salinity-biofertility interactive effects on proline, glycine and various antioxidants in Lactuca sativa. Plant Omics J. 2009; 2:197-205.
- Younis ME, Tourky SMN, Elsharkawy SEA. Symptomatic parameters of oxidative stress and antioxidant defense system in Phaseolus vulgaris in response to copper or cadmium stress. S Afr J Bot. 2018; 117:207-214.
- Yurekli F, Porgali ZB. The effects of excessive exposure to copper in bean plants. Acta Biol Crac Ser Bot. 2006 ; 48: 7 – 13.
- Zeid IM, Abou El Ghate HM. Response of bean to some heavy metals in sewage water. Pak J Biol Sci. 2007; 7: 34-40.
- Zengin F, Munzuroglu O. Effect of some heavy metals on content of chlorophyll, proline and some antioxidant chemicals in bean (Phaseolus vulgaris L.) seedlings. Acta Biol Crac Ser Bot. 2005 ; 47:157–164.
This work and its PDF file(s) are licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.