Research article of Journal of Plant and Environmental Research
Phytostabilzation as a sustainable phytoremediation strategy for lead contaminated soil – Screening of biofuel plants for lead tolerance and accumulation
Hira Amin1*, Basir Ahmed Arain1, Taj Muhammad Jahangir2, Abdul Rasool Abbasi3, Muhammad Sadiq Abbasi4, Farah Amin5
1Institute of Plant Sciences, University of Sindh, Jamshoro 76080 – Pakistan; 2Institute of Advanced Research Studies in Chemical Sciences, University of Sindh, Jamshoro 76080 – Pakistan; 3Department of Fresh Water Biology and Fisheries, University of Sindh, Jamshoro 76080 – Pakistan; 4Department of Mathematics & Statistics, Quaid-e-Awam University of Engineering, Science & Technology Nawabshah 67480 – Pakistan 5National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080 – Pakistan
The contamination of soil by lead has one of the major environmental problems globally. In present study, the experiment was carried out for lead contaminated soil with four plant species i.e., A. esculentus, A. sativa, G. abyssinica and G. max that were subjected to six lead concentrations i.e., 100, 200, 400, 600, 800 and 1000 mg Pb kg-1 soil. Soil without spiked were taken as control and investigated for lead phytotoxicity, tolerance and accumulation. After 12 weeks of experiment, lead toxicity on growth and biochemi-cal parameters were determined. For four plant species, seed germination and most of the growth parameters were significantly (p<0.05) reduced under high lead stress. Chloro-phyll contents were also decreased with increased lead concentrations. Accumulation of lead was higher in roots than shoots of all studied plants. Among the four plant species, significant highest lead accumulation was found in the roots and shoots of A. sativa. Bio-concentration factor, bioaccumulation coefficient, translocation factor and phytoremdia-tion ratios were suggested that A. sativa with high lead tolerance and accumulation capac-ity has considered an efficient plant for the reclamation of lead contaminated soil.
Keywords: Lead; toxicity; tolerance; accumulation; phytoremediation efficiency.
How to cite this article:
Hira Amin, Basir Ahmed Arain, Taj Muhammad Jahangir, Abdul Rasool Abbasi, Muhammad Sadiq Abbasi, Farah Amin. Phytostabilzation as a sustainable phytoremediation strategy for lead contaminated soil – Screening of biofuel plants for lead tolerance and accumulation . Journal of Plant and Environmental Research, 2020,4:18
1. Adesodun JK, Atayese MO, Agbaje T, Osadiaye BA, Mafe O, Soretire AA (2010 Phytoremediation potentials of sunflowers (Tithonia diversifolia and Helianthus annuus) for metals in soils contaminated with zinc and lead nitrates. Water Air Soil Pollut. 207: 195–201.
2. Alvarez PJJ, Illman WA (2006) Bioremediation and Natural Attenuation: Process Fundamentals and Mathematical Models. Wiley-Interscience, New Jersey.
3. Arias JA, Peralta-Videa JR, Ellzey JT, Ren M, Viveros MN, Gardea-Torresdey JL (2010) Effects of Glomus deserticola inoculation on Prosopis: enhancing chromium and lead uptake and translocation as confirmed by X-ray mapping, ICP-OES and TEM techniques. Environ Exp Bot 68(2):139–148.
4. Arnon DI (1949) Copper enzymes in isolated chloroplasts: polyphenol oxidase in Beta vulgaris. Plant Physiol 24:1–15.
5. Audet P, Charest C (2007) Heavy metal phytoremediation from a meta-analytical perspective. Environ. Pollut. 147: 231–237.
6. Awokunmi EE (2016) The Potential of Abelmoschus esculentus in EDTA-Asssisted Phytoextraction of Heavy Metals from Soil of Bashiri Dumpsite, Ado Ekiti, Nigeria, International Journal of Environmental Protection, 6: 9 – 14.
7. Becerril Soto, JM, Barrutia Sarasua O, García Plazaola JI, Hernández A, OlanoMendoza JM, Garbisu Crespo C (2007) Especies nativas de suelos contaminados por metales: aspectos ecofisiológicos y su uso en fitorremediación. Ecosistemas 16: 50–55.
8. Betancur, L.M.A., Mazo, K.I.M., Mendoza, A.J.S., 2005. Fitorremediación: la alternativa para absorber metales pesados de los biosólidos. Revista Lasallista de Investigación 1: 57–60.
9. Cecchi M, Dumat C, Alric A, Felix-Faure B, Pradere P, Guiresse M (2008) Multi-metal contamination of a calcic cambisol by fallout from a lead-recycling plant. Geoderma 144: 287–298.
10. Cenkci S, Cigerci IH, Yildiz M, Özay C, Bozdag A, Terzi H (2010) Lead contamination reduces
11. chlorophyll biosynthesis and genomic template stability in Brassica rapa L. Environ Exp Bot 67: 467–473.
12. Ciura J, Poniedziałek M, Sękara A, Jędrszczyk E (2005) The possibility of using crops as metal phytoremediants. Polish Journal of Environmental Studies, 14: 17–22.
13. Cui S, Zhou Q, Chao L (2007) Potential hyperaccumulation of Pb, Zn, Cu and Cd in endurant plants distributed in an old smeltery, northeast China. Environ Geol. 51:1043 – 1048.
14. Fabietti G, Biasioli M, Barberis R, Ajmone-Marsan F (2009) Soil contamination by organic and inorganic pollutants at the regional scale: the case of Piedmont, Italy. J. Soils Sediment. 10: 290–300.
15. Fanrong Z, Shafaqat A, Haitao Z, Younan O, Boyin Q, Feibo W, Guoping Z (2011) The influence of pH and organic matter content in paddy soil on heavy metal availability and their uptake by rice plants, Environmental Pollution 159: 84 – 91.
16. Fitz WJ, Wenzel WW (2002) Arsenic transformation in the soil-rhizosphere- plant system, fundamentals and potential application of phytoremediation. J Biotechnol 99: 259–278.
17. Garbisu, C., Alkorta, I., 2003. Basic concepts on heavy metal soil bioremediation. Eur. J. Miner. Process. Environ. Prot. 3: 58–66.
18. Gopal R, Rizvi AH (2008) Excess lead alters growth, metabolism and translocation of certain nutrients in radish. Chemosphere 70: 1539–1544.
19. Gupta D, Huang H, Yang X, Razafindrabe B, Inouhe M (2010) The detoxification of lead in Sedum alfredii H. is not related to phytochelatins but the glutathione. J Hazard Mater 177: 437–444
20. Hanen Z, Tahar G, Abelbasset L, Rawdha B, Rim G, Majda M, Souhir S, Stanley L, Chedly A (2010) Comparative study of Pb-phytoextraction potential in Sesuvium portulacastrum and Brassica juncea: tolerance and accumulation. J Hazard Mater 183: 609 – 615.
21. Hernandez L, Probst A, Probst JL, Ulrich E (2003) Heavy metal distribution in some French forest soils: evidence for atmospheric contamination. Sci. Total Environ. 312: 195–219.
22. Kopittke PM, Asher CJ, Kopittke RA, Menzies NW (2007) Toxic effects of Pb2+ on growth of cowpea (Vigna unguiculata). Environ Pollut 150(2):280–287
23. Li MS, Luo YP, Su ZY (2007) Heavy metal concentrations in soils and plant accumulation in a restored manganese mine land in Guangxi, South China. Environ Pollut. 147: 168 – 175.
24. Lone MI, He Z, Stoffella PJ, Yang X (2008) Phytoremediation of heavy metal polluted soils and water: progresses and perspectives. J. Zhejiang Univ. – Sci. B 9: 210–220.
25. Maestri E, Marmiroli M, Visioli G, Marmiroli N (2010) Metal tolerance and hyperaccumulation: costs and trade-offs between traits and environment. Environ Exp Bot 68:1–13
26. McGrath SP, Zhao FJ (2003) Phytoextraction of metals and metalloids from contaminated soils. Curr Opin Biotech. 14: 561 – 572.
27. Memon AR, Schroder P (2009) Implications of metal accumulation mechanisms to phytoremediation. Environ. Sci. Pollut. Res. 16, 162 – 175.
28. Mendez, MO, Maier RM (2008) Phytostabilization of mine tailings in arid and semiarid environments—an emerging remediation technology. Environment Health Perspective, 116: 278–283.
29. Monni S, Salemma M, Millar N (2000) The tolerance of Empetrum nigrum to copper and nickel. Environ Pollut 109: 221 – 229.
30. Navarro-Aviñó J, Aguilar A, López-Moya J (2007) Aspectos bioquímicos y genéticos de la
31. tolerancia y acumulación de metales pesados en plantas. Ecosistemas 16: 10–25.
32. Odjegba VJ, Fasidi IO (2007) Phytoremediation of heavy metals by Eichhornia crassipes. Environmentalist 27: 349–355.
33. Oh K, Li T, Cheng HY, Xie Y, Yonemochi S (2013) Development of profitable phytoremediation of contaminated soils with biofuel crops. J. Environ. Prot. 4: 58–64.
34. Pais I, Jones JB (2000) The handbook of trace elements. Saint Lucie Press, Boca Raton, FL, p 223.
35. Pourakbar L, Khayami M, Khara J, Farbidina T (2007) Physiological effects of copper on some biochemical parameters in Zea mays L. seedlings. Pak J Biol Sci 10: 4092 – 4096.
36. Punamiya P, Datta R, Sarkar D, Barber S, Patel M, Das P (2010) Symbiotic role of glomus mosseae in phytoextraction of lead in vetiver grass [Chrysopogon zizanioides (L.)]. J Hazard Mater 177: 465–474
37. Rachit K, Verma KS, Meena T, Yashveer V, Shreya H (2016) Phytoextraction and Bioconcentration of Heavy Metals by Spinacia oleracea Grown in Paper Mill Effluent Irrigated Soil, Nature Environment and Pollution Technology, 15: 817 – 824.
38. Raskin I, Ensley BD (2000) Phytoremediation of toxic metals: Using plants to clean up the environment, Wiley, New York.
39. Rohan D, Mayank V, João P, Paul MS (2013) Spatial distribution of heavy metals in soil and flora associated with the glass industry in North Central India: implications for phytoremediation. Soil Sediment Contam Int J 22: 1 – 20.
40. Sakakibara M, Ohmori Y, Ha NTH, Sano S, Sera K (2011) Phytoremediation of heavy metal contaminated water and sediment by Eleocharis acicularis. Clean: Soil, Air, Water 39: 735–741.
41. Salt DE, Smith RD, Raskin I (1998) Phytoremediation. Annu Rev Plant Phys 49: 643 – 668.
42. Sengar RS, Gautam M, Sengar RS, Sengar RS, Garg SK, Sengar K, Chaudhary R (2008) Lead stress effects on physiobiochemical activities of higher plants. Rev Environ Contam Toxicol 196:1–21.
43. Seregin TV, Ivanov VB (2001) Physiological aspects of toxin action of cadmium and lead on high plants. Plant Physiol 48: 606 – 630.
44. Shabani N, Sayadi MH (2012) Evaluation of heavy metals accumulation by two emergent macrophytes from the polluted soil: an experimental study. Environmentalist 32: 91–98.
45. Shahid M, Pinelli E, Pourrut B, Silvestre J, Dumat C (2011) Lead-induced genotoxicity to Vicia faba L. roots in relation with metal cell uptake and initial speciation. Ecotoxicol Environ Saf 74: 78–84.
46. Sharma S, Singh B, Manchanda VK (2014) Phytoremediation: role of terrestrial plants and aquatic macrophytes in the remediation of radionuclides and heavy metal contaminated soil and water. Environ. Sci. Pollut. Res. 22: 946–962.
47. Srinivasan M, Sahi SV, Paulo JCF, Venkatachalam P (2014) Lead heavy metal toxicity induced changes on growth and antioxidative enzymes level in water hyacinths [Eichhornia crassipes (Mart.)], Botanical Studies 2014, 55:54
48. Sun YB, Zhou QX, Wang L, Liu WT (2009) The influence of different growth stages and dosage of EDTA on Cd uptake and accumulation in Cd-hyperaccumulator (solanium nigrum L.), Bull. Environ. Contam. Toxicol., 82: 348 – 353.
49. Talebi S, Nabavi KSM, Sohani DAL (2014) The study effects of heavy metals on germination characteristics and proline content of Triticale (Triticoseale Wittmack). Intl J Farm & Alli Sci 3: 1080 – 1087.
50. Tian SK, Lu LL, Yang XE et al (2010) Spatial imaging and speciation of lead in the accumulator plant Sedum alfredii by microscopically focused synchrotron X-ray investigation. Environ Sci Technol 44: 5920–5926.
51. Tong YP, Kneer R, Zhu YG (2004) Vacuolar compartmentalization: a secondgeneration approach to engineering plants for phytoremediation. Trends Plant Sci. 9: 7 – 9.
52. Van Aken B (2009) Transgenic plants for enhanced phytoremediation of toxic explosives. Curr. Opin. Biotechnol. 20: 231–236.
53. Vymazal J (2016) Concentration is not enough to evaluate accumulation of heavy metals and nutrients in plants. Sci Total Environ 544:495–498.
54. Wilkins, DA (1978) The measurement of tolerance to edaphic factors by means of root growth. New Phytol. 80: 623–633
55. Wuana RA, Okieimen FE (2011) Heavy metals in contaminated soils: a review of sources, chemistry, risks and best available strategies for remediation. ISRN Ecology, 1–20.
56. Yoon J, Cao X, Zhou Q, Ma LQ (2006) Accumulation of Pb, Cu and Zn in native plants growing on a contaminated Florida site. Sci Total Environ. 368: 456 – 464.
57. Yue-bing S, Qixing Z, Jing A, Wei-tao L, Rui L (2009) Chelator-enhanced phytoextraction of heavy metals from contaminated soil irrigated by industrial waste water with the hyperaccumulator plant (Sedum alfredii Hence), Geoderma, vol. 150: 105 – 112.
58. Zheng LJ, Liu XM, Lutz-Meindl U, Peer T (2011) Effects of lead and EDTA-assisted lead on biomass, lead uptake and mineral nutrients in Lespedeza chinensis and Lespedeza davidii. Water Air Soil Poll 220:57–68.