Review Article of Journal of Pharmaceutical Research and Reviews
Electrochemical study of methimazole and its direct determination in pharmaceutical preparations and human serum by square wave and differential pulse voltammetry
Bilal Yilmaz1 , Fatma Bayrakceken Nisanci2*
1Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, 25240, Erzurum, Turkey;
2Department of Chemistry, Faculty of Science, Ataturk University, 25240, Erzurum, Turkey
Two simple and rapid methods were developed for the determination of methimazole in pharmaceutical preparations and human serum by square wave and differential pulse voltammetry. The anodic peak at +0.60 V obtained in a buffer on glassy carbon electrode was used for analysis. The peak current and peak potential depend on pH, scan rate and initial potential. Decrease of the anodic peak with increasing pH, as well as deviations from linear plots of ip = f(C) and ip = f(C) and ip = kv1/2 indicate that this peak at higher concentrations is affected by adsorption-desorption phenomena. The linearity was established over the concentration range of 10-80 ug/mL for both methods in supporting electrolyte and human serum. The repeatability and reproducibility of the methods for all media (such as supporting electrolyte and serum samples) were determined. Precision and accuracy were also checked in all media. Developed methods in this study can be applied to Thyromazol tablet. Also, the proposed techniques were successfully applied to spiked human serum samples. No electroactive interferences from the endogenous substances were found in the serum samples.
Keywords: Methimazole, serum, voltammetry, tablet
How to cite this article:
Bilal Yilmaz and Fatma Bayrakceken Nisanci.Electrochemical study of methimazole and its direct determination in pharmaceutical preparations and human serum by square wave and differential pulse voltammetry. Journal of Pharmaceutical Research and Reviews, 2017; 1:5.
 Aletrari, M.; Kanari, P.; Partassides, D.; Loizou, E. Study of the British Pharmacopeia method on methimazole (thiamazole) content in carbimazole tablets. J. Pharm. Biomed. Anal. 1998, 16, 785-792.
 Antithyroidagents, Martindale, in: Reynolds JEF. (Ed.),The ExtraPharmacopoeia, 29th ed, The Pharmaceutical Press, London, 1989, pp.682-688.
 Aboul-Enein, H.Y.; Al-Badr, A.A. in: Forey K. (Ed.), Analytical Profiles of Drug Substance, 18, Academic Press, NewYork, 1979, pp.351-371.
 Weetman, A.P.; McGregor, A.M.; Hall, R.E. Evidence for an effect of antithyroid drugs on the natural history of Graves disease. Clin. Endocrinol. 1984, 21, 163-172.
 Nikolic, K.; Velasevic, K. Conductometric determination of captopril. Pharmazie 1987, 42, 698-
 Berka, A.; Velasevic, K.; Nikolic, K. Conductometric determination of methimazole. Pharmazie
1989, 44, 499-499.
 Moretti, G.; Betto, P.; Cammarata, P.; Fracassi, F.; Giambenedetti, M.; Borghese, A. Determination of thyreostatic residues in cattle plasma by high-performance liquid chromatography with ultraviolet detection. J. Chromatogr. B, 1993, 616, 291-296.
 Elbardicy, M.G.; Elsaharty, Y.S.; Tawakkol, M.S. Determination of carbimazole and methimazole
by first and third derivative spectrophotometry. Spectrosc. Lett. 1991, 24, 1079-1095.
 Sanchezpedreno, C.; Albero, M.I.; Garcia, M.S.; Rodenas, V. Flow-injection spectrophotometric determination of carbimazole and methimazole. Anal. Chim. Acta 1995, 308, 457-461.
 Garcia, M.S.; Albero, M.I.; Sanchezpedreno, C.; Tobal, L. Kinetic determination of carbimazole, methimazole and propylthiouracil in pharmaceuticals, animal feed and animal livers. Analyst 1995, 120,129-133.
 Pinzanti, S.; Papeschi, G.; LaPorta, E. Potentiometric titration of thiols, cationic surfactants and halides using a solid state silver silver sulphide electrode. J. Pharm. Biomed. Anal. 1983, 1, 47-53.
 Zhang, S.; Sun, W.; Zhang, W.; Qi, W.; Jin, L.; Yamamoto, K.; Tao, S.; Jin, J. Determination of thiocompounds by liquid chromatography with amperometric detection at a nafion/indium hexacyanoferrate film modified electrode. Anal. Chim. Acta 1999, 386, 21-30.
 Wang, A.; Zhang, L.; Zhang, S.; Fang, Y. Determination of thiols following their separation by CZE with amperometric detection at a carbon electrode. J. Pharm. Biomed. Anal. 2000, 23, 429-
 Aslanoglu, M.; Peker, N. Determination of methimazole in pharmaceutical preparations using an
HPLC method coupled with an ıodine-azide post-column reaction. J. Pharm. Biomed. Anal. 2003, 33, 1143-1147.
 Yazhen, W. Electrochemical determination of methimazole based on the acetylene black/ chitosan
film electrode and its application to rat serum samples. Bioelectrochem. 2011, 81, 86-90.
 Xi, X.; Ming, L.; Liu, J. Electrochemical determination of thiamazole at a multi-wall carbon
nanotube modified glassy carbon electrode. J. Appl. Electrochem. 2010, 40, 1449-1454.
 Shahrokhian, S.; Ghalkhani, M. Voltammetric determination of methimazole using a carbon paste electrode modified with a schiff base complex of cobalt. Electroanalysis 2008, 20, 1061-1066.
 Swartz, M.E.; Krull, I.S. Analytical Method Development and Validation, Marcel Dekker, New York, 1997.
 Wang, J. (Ed.), Analytical Electrochemistry, 2nd ed., Wiley/VCH Publishers, New York (2000).
 Kissenger, P.T.; Heineman, W.R. (Eds.), Laboratory Techniques in Electroanalytical Chemistry, 2nd ed., Marcel Dekker, New York, 1996.
 Wang, J. (Ed.), Electroanalytical Techniques in Clinical Chemistry and Laboratory Medicine, VCH Publishers, New York, 1996.
 The European Agency for the Evaluation of Medicinal Products. ICH Topic Q2B Note for Guideline on Validation of Analytical Procedures: Methodology GPMP/ICH/281/95, 1996.
 El-Hefnawey, G.B.; El-Hallag, I.S.; Ghoneim, E.M.; Ghoneim, M.M. Voltammetric behavior and quantification of the sedative-hypnotic drug chlordiazepoxide in bulk form, pharmaceutical formulation and human serum at a mercury electrode. J. Pharm. Biomed. Anal. 2004, 34, 75-86.
 Corti, P.; Corbini, G.; Gratteri, P.; Furlanetto, S.; Pinzauti, S. Determination of some quinolones in tablets, human plasma and urine by differential-pulse polarography. Int. J. Pharm. 1994, 111, 83-87.
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