Research Article of Journal of Pharmaceutical Research and Reviews
Effect of Ricinodendron heudelotii seed extract on the oxidative stress biomarkers of Diabetic albino rats
Odinga, T.1,2, Nwaokezi, C.O.1
1Department of Biochemistry, Rivers state university. Nigeria;
2Department of Biochemistry, University of Port Harcourt, Rivers state, Nigeria.
This study evaluated the potentials of the seed extract of Ricinodendron heudelotii on the oxidative stress biomarkers of diabetic albino rats of the wistar strain. Diabetes mellitus was induced via intraperitoneal administration of 160 mg/ kg bodyweight Alloxan monohydrate in normal saline and confirmed after 120 hours of blood glucose level above 300mg/dl. Blood samples were collected from the animals and analyzed for Malondialdehyde, Catalase, Reduced Glutathione, Glutathione-S-transferase, Glutathione peroxidase, and Superoxide dismutase activities as biomarkers of oxidative stress. The result revealed a significant decrease at P≤0.05 in blood glucose concentration of diabetic rats with increase in the seed extract concentration, Malondialdehyde (MDA) decreased significantly in the diabetic rats while Catalase increased significantly at P≤0.05 as the extract concentration increased. Variations in Glutathione-S-transferase, Glutathione Peroxidase, Superoxide dismutase and Reduced Glutathione in comparison with control was observed. The results therefore suggests that the aqueous seed extract of Ricinodendron heudelotii has the potency to reduce glucose level and act as antioxidants against oxidative stress, hence its use therapeutically.
Keywords: Ricinodendron heudelotii, Alloxan monohydrate, Antioxidant biomarkers, Diabetes mellitus, oxidative stress.
How to cite this article:
Odinga, T., Nwaokezi, C.O. Effect of Ricinodendron heudelotii seed extract on the oxidative stress biomarkers of Diabetic albino rats. Journal of Pharmaceutical Research and Reviews, 2020; 4:19. DOI: 10.28933/jprr-2019-11-1905
1. Abdel-Hassan, I. A., Abdel-Barry, J. A. and Mohammed, S. T. (2001). The hypoglycemic and anti-hyperglycemic effect of Citrullus colocynthis fruit aqueous extract in normal and alloxan diabetic rabbits. Journal of Ethnopharmacology. 71; 325-330.
2. Adrogue, H. J., Ledere, E. D., Suki, W. N. and Eknoyan, G. (1986). Determination of plasma potassium levels in diabetic ketoacidiosis. Medicine 65:163-172.
3. Al-Hader, A. A., Hasan, Z. A. And Agel, M. B. (1994). Hypoglycemic and insulin release inhibitory effect of Rosemorinus officialis. Journal of Ethnopharmacology. 43; 217-222.
4. Beutler, E., Duron, O. and Kelly, B. M.(1963). Improved method for the determination blood glutathione. J. Lab. Clin Med. 61:882-8. PMid:13967893.
5. Edwin, H., Keyvan, K. G., Chia-Chi, L., Ravi, B. and Gemma, A. F. (2013). Biological markers of oxidative stress: Applications to cardiovascular research and practice. Redox Biology 1(1): 483-491
6. Firdous, S. M. (2014). Phytochemical for treatment of diabetes. Excli Journals. 13; 451-453.
7. Gawel, S., Wardas, M., Nieedworok, E. and Wardas, P. (2004). Malondialdehyde as a lipid peroxidation marker. Pub Med. 57(9-10); 453-455.
8. Griendling, K. K. and FitzGerald, G.A. (2003). Oxidative stress and cardiovascular injury: Part I: basic mechanisms and in vivo monitoring of ROS. Circulation 108; 1912–1916.
9. Harrison, D., Griendling, K. K., Landmesser, U., Hornig, B. and Drexler, H. (2003). Role of oxidative stress in atherosclerosis. American Journal of Cardiology. 91;7A–11A.
10. Johnson, J. T., Iwang, E. U., Hemen, J. Z., Odey, M. O., Effiong, E. E. and Eteng, O. E. (2012). Evaluation of anti-nutrient contents of watermelon (Citrullus lanatus). Ann Biol Res. 3(11):5145-5150.
11. Keller, T., Zeller, T., Peetz, D., Tzikas, S., Roth, A., Czyz, E., Bickel, C., Baldus, S., Warnholtz, A., Frohlich, M., Sinning, C. R., Eleftheriadis, M. S., Wild, P. S., Schnabel, R. B., Lubos, E., Jachmann, N., Genth-Zotz, S., Post, F., Nicaud, V., Tiret,VL., Lackner, K. J., Munzel, T. F. and Blankenberg, S, (2009). Sensitive troponin I assay in early diagnosis of acute myocardial infarction. New England Journal of Medicine 361; 868–877.
12. Marklund, S. and Marklund, G. (1974). Involvement of superoxide anion radical in the au¬toxidation of pyrogallol and a convenient assay of superoxide dismutase. Eur. J. Biochem. 47(3); 469-73.
13. Mueller, C. F., Laude, K., McNally, J. S. and Harrison, D. G. (2005). ATVB infocus: redox mechanisms in blood vessels, Arteriosclerosis, Thrombosis, and Vascular Biology 25;274–278.
14. Nandi, A. and Chatterjea, I. B. (1988). Assay of superoxide dismutase activity in animal tis¬sues. J. Bio sci. 13(3); 305-15.
15. Odinga, T., Essien, E. B. and Akaninwor, J. O. (2018). Prophylactic potency of Ricinodendron heudelotii seeds against aspirin-induced ulcer. Research journal of Life sciences, Bioinformatics, pharmaceuticals and Chemical sciences. 4(6); 664-671.
16. Odinga, T., Worlu-Wodu, Q. E. and Deekae, S. (2016) Bioprospective Screening of Ricinodendron heudelotii Seeds. J Anal Pharm Res 3(7): 00084. DOI: 10.15406/japlr.2016.03.00084
17. Pompella, A., Visvikis, A., Paolicchi, A. L. and De Tata, V. (2003). The changing face of Glutathione, a cellular protagonist. Biochemical Pharmacology. 66(8); 1499-503.
18. Sedighi, O., Makhlough, A., Shokrzadeh, M. and Hoorshad, S. (2014). Association between Selenium and Glutathione peroxidase levels and severity of Diabetic Nephropathy in patients with type 2 Diabetes Mellitus. Nephro-urology monthly. 6(5); e21355.
19. Shlafer, M. and Marieb, E. (1989). The Nurse, Pharmacology and drug therapy. Addison Wesley, Menlopark, California.
20. Singh, W. L. (2011).Traditional medicinal plants of Manipur as anti-diabetics. J. Med. Plants Res. 5(5), 677-687.
21. Slatter, D. A., Bolton, C. H. and Bailey, A. J. (2000). The importance of lipid-derived malondialdehyde in diabetes mellitus. Diabetologia 43(2000); 550–557.
22. Somani, R. S., Jaina, K. S. and Singhai, A. K. (2007). Hypoglycemic activity of roots of Rubla cordifolla in normal and diabetic rats. Pharmacologyonline 1:162-169.
23. Satoh, K. (1978). Serum lipid peroxidation in cerberovascular disorders determined by new colorimetric method. Clin. Chem. ACTA. 90:37-43. https://doi. org/10.1016/0009-8981 (78)90081-5.
24. Uchida, K. (2000).Role of reactive aldehyde in cardiovascular diseases, Free Radical Biology and Medicine. 28(2000)1685–1696.
25. Wang, T. J. (2011). Assessing the role of circulating, genetic, and imaging biomarkers in cardiovascular risk prediction, Circulation 123; 551–565.