Research Article of Research Journal of Pharmacology and Pharmacy
Glycemic control and electrolyte changes in diabetic rats treated with pioglitazone
*1Adeyomoye O.I and 2Adeola W.F
1 Department of Physiology, University of Medical Sciences, Ondo, Ondo State, Nigeria
2Department of Physiology, Olabisi Onabanjo University, Ago-Iwoye Ogun State, Nigeria
This study evaluated the effect of pioglitazone on glucose and serum electrolytes in normal and diabetic rats. Fifteen adult wister rats were divided into 3groups (n=5). Group 1 and 2 served as the normal control and diabetic untreated group respectively and each received 0.3ml distilled water. Group 3 served as diabetic treated with 4mg/kg pioglitazone. This study lasted for twelve days after which blood samples were collected under mild anesthesia through retro-orbital sinus and centrifuge to obtain serum for analysis. The weight and fasting blood glucose level of rats were determined using the weighing scale and glucose oxidase method respectively. The serum electrolytes (Na+, K+, and Ca2+) were determined using the flame photometry method. Results showed significant increase in (p<0.05) in weight of pioglitazone treated group when compared with the normal control and diabetic untreated group. The average weight gain in pioglitazone treated group (29.80%) was significantly higher when compared with previous studies (5%). There was significant decrease (p<0.05) in fasting blood glucose level in pioglitazone treated group when compared with diabetic untreated group. Na+ concentration was significantly higher (p<0.05) in pioglitazone treated group when compared with diabetic untreated group. A significant increase (p<0.05) in Ca2+ concentration was observed in pioglitazone treated group when compared with normal control and diabetic untreated groups. In conclusion, oral administration of pioglitazone caused weight gain, hypernatremia, hypercalcemia and a decrease fasting blood glucose level. Pioglitazone is therefore useful in glycemic control and maintainance of electrolyte balance in diabetic mellitus.
Keywords: Pioglitazone, glycemic control, hypernatremia, hypercalcemia, serum electrolytes.