Article of Research Journal of Pharmacology and Pharmacy
Genetic Polymorphism of Tumor Necrosis Factor (Tnf – Α) and Their Association With Dengue Infection
SANTOS, A.C.M.; MOURA, E.L.; SANTOS, B.R.C.; FERREIRA, J.M.; FIGUEIREDO, E.V.M.S.
Federal University of Alagoas
Some studies have associated the genetic constitution of the host with the clinical manifestations of patients infected with dengue. The objective of this study was to investigate the association of polymorphisms SNP -308A / G promoter of the TNF-α gene region with the clinical and laboratory manifestations of dengue in Alagoas Agreste population. The genotypes of the subjects were identified by PCR in real time. Laboratory data and symptoms were collected from medical records of patients. Statistical analysis was performed with SPSS 20.0 Software. The study wasapproved by the Ethics Committee of the Federal University of Alagoas (report number 405940). Of the 67 patients, 58.2 % were female (n = 39), but without statistical difference (p = 0.179), mean age was 31.5 (SD ± 13.2). With regard to clinical manifestations, 86.6 % (n = 58) had fever, 80.6 % (n = 54) were headache, 55.2 % (n = 37) developed myalgia The genotypic profile of the TNF-α patients It was 3 % (N = 2) patients A / A, 17.9 % A / G (n = 12) and 79.1 % G / G (n = 53). Genotypic compared the clinical characteristics were not significantly associated. Since in comparison to the laboratory results, a significant association of G / G genotype with hematocrit (p = 0.02) was observed, hemoglobin (p> 0.001) and erythrocytes (p> 0.001). There was no association between other genotypes or between leukocytes (p = 0.13) and platelets (p = 0.39) with the G / G genotype, while 61.2 % of patients in this profile have submitted leukocyte levels below 4,000 / mm³. Of the patients, 58.2 % were women, results similar to work nacionais, as well as the clinical manifestation of febre. A study in Puerto Rico found that 71 % of patients had cefaleia7 in line with this study. The G/G genotype was prevalent in our cases, as well as in Rio de Janeiro population. This genotype seems to have implications on the variation of hematocrit, red blood cells and hemoglobin in our population, the same genotype showed protection against disease progression in Venezuela when combined with other SNPs. Fever and headache are the most common symptoms in our patients. The G / G genotype may be associated with variations in the values of blood components in our population.
Keywords: Dengue; Polymoprhism; TNF; Health research
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