Biogenic synthesis of Adhatoda vasica L. Nees mediated silver nanoparticles

Biogenic synthesis of Adhatoda vasica L. Nees  mediated silver nanoparticles and their antibacterial, anticancer activity on Hep-G2 cell lines

 International Journal of Traditional and Complementary MedicineArumugam Sengottaiyan 1,*†, Chinnappan Sudhakar 1†, Kandasamy Selvam 2*, Thangaswamy Selvankumar 1, Muthusamy Govarthanan 1,3, Balakrishnan Senthikumar 2 and Koildhasan Manoharan 4

1 PG & Research Department of Biotechnology, Mahendra Arts and Science College (Autonomous), Kalippatti, Namakkal 637501, Tamil Nadu, India. 2 Centre for Biotechnology, Muthayammal College of Arts and Science, Rasipuram, Namakkal 637 408, Tamil Nadu, India 3 Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570752, South Korea 4 Raja Duraisingam Government Arts & Science College, Sivagangai.

The first two authors equally contributed this work

The present investigation has been studied with the green synthesis of silver nanoparticles (AgNPs) using medicinally valued Adhatoda vasica -Nees and to evaluate the antibacterial and anticancer activity against HEP-G2 (Human epithelium cells of liver cancer) cell lines. The UV-Vis spectroscopy results show a strong resonance centered on the surface of silver nanoparticles at 420 nm. Fourier transform infrared (FT-IR) spectroscopy study demonstrates A. vasica aqueous extract acted as the reducing and stabilizing agent during the synthesis. The X-ray diffraction (XRD) analysis confirmed that the synthesized AgNPs are single crystalline face-centered cubic in structure, average crystal size 21 nm. Scanning electron microscope–energy dispersive spectroscopy (SEM-EDS) image confirmed synthesis of relatively uniform nanoparticles. The EDS analysis of the nanoparticles dispersion, using a range of 2-4 keV, confirmed the presence of elemental silver, without any contamination. The antibacterial activities were carried out against pathogenic bacteria. The maximum zone of inhibition was observed in the synthesized AgNPs (10µg/mL) against Staphylococcus sp. (16mm), Klebsiella sp. (14.5mm). The cytotoxicity activity as evidence by MTT assay with HEP-G2 cell lines. The synthesized AgNPs are ready for the application in the field of nanomedicine against pathogenic bacteria and very good anticancer drug.

Keywords: Adhatoda vasica, silver nanoparticles, antibacterial, cytotoxicity, HEP-G2 cell lines.

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Arumugam Sengottaiyan, Chinnappan Sudhakar, Kandasamy Selvam, Thangaswamy Selvankumar, Muthusamy Govarthanan, Balakrishnan Senthikumar and Koildhasan Manoharan. Biogenic synthesis of Adhatoda vasica L. Nees  mediated silver nanoparticles and their antibacterial, anticancer activity on Hep-G2 cell lines. International Journal of Traditional and Complementary Medicine 2017, 2:14. DOI:10.28933/sengottaiyan-ijtcm-201604-2

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