American Journal of Histology and Cytology


Research Article of American Journal of Histology and Cytology STUDY OF PLACENTAS OF HYPERTENSIVE MOTHERS ASSISTED IN TWO PUBLIC MATERNITIES OF RECIFE-PE Karinne Gomes Mendonça1*, Evandro Valentim da Silva2, Fálba Bernadete Ramos dos Anjos3 1UFPE, 2Pathology Laboratory of Hospital das ClínicasHC/UFPE, 3Professor / Researcher at the Department of Histology and Embryology – UFPE Objective: Perform the histomorphometric study of the following regions: areas of perivillous fibrin deposition; thickness of the endothelial layer of the vessels; analysis of Tenney-Parker changes. Analyze the Placental Index (IP). Results: It was seen that the weight of the placentas increased according to the weight of the newborn, however, when the variable PI was analyzed as being decisive for the development of a risk-free pregnancy, it was found that this parameter was not significant between the groups studied. (p <0.05). It was found that there is a significant difference in relation to the areas of fibrin deposition (P <0.05) between the groups and that it possibly converges with the data found in the literature in which it could be established that in the GrH there is a different relationship in this pattern. The findings of the thickness of the middle layer were significant in the GrH when compared to the GrN (p <0.05), when this parameter is compared between the Hypertensive Disorders of Pregnancy (HDP) groups, microscopically the PE cases showed prominent changes. I n the present study, Tenney-Parker changes were prominent and discreet in 75% of the GrH placentas versus 20% in the GrN. However, there are contradictions in the literature regarding the consideration of this finding seen only in Hematoxylin-Eosin staining. Conclusion: Given the above, the presence of syncytial knots, the difference between vessel thickness and areas of fibrin deposition are possibly involved in the pathogenesis of Hypertensive Syndromes, regardless of age and established classifications ...


Review Article of American Journal of Histology and Cytology ADVANCES IN THE IDENTIFICATION TECHNIQUES FOR PATHOGENIC MICROORGANISMS Lívia Caroline Alexandre de Araújo1; Rafael Artur de Queiroz Cavalcanti de Sá2; Sivoneide Maria da Silva2; Francisco Henrique da Silva2; Cíntia Justino Rabelo3; Mariana Gomes Vidal Sampaio3; 1 Centro de Ciências Agrárias, Universidade Federal da Paraíba – CCA/UFPB 2 Centro de Ciências da Saúde, Universidade Federal de Pernambuco – CCS/UFPE 3 Centro Universitário Católica de Quixáda - UNICATÓLICA Objectives: The research objective was to present the main techniques for identifying pathogenic microorganisms and the application of new technologies for the clinical diagnosis of infectious diseases. Methods: It was made a search for free and recent journals available online in the databases of Pubmed (National Library of Medicine and the National Institutes), Lilacs (Latin American and Caribbean Literature in Health Sciences), and Scielo (Scientific Electronic Library Online), based on keywords related to the proposed theme. Results: From the researched literature, it was possible to verify that conventional techniques, despite their limitations, are still widely used for the identification and microbial characterization. However, in the last decades, molecular methods have been widely inserted in the laboratory routine seeking to increase the capacity to detect infectious agents with high sensitivity, specificity, speed, and low cost. Among the various techniques, amplification of DNA sequences is highlighted by the polymerase chain reaction (PCR), the sequencing of the 16S rRNA gene, and other variations of PCR. In addition to these, new technologies have been developed, such as new generation sequencing (NGS) and Matrix-assisted Laser Desorption/ionization-time-of-flight Mass Spectrometry (MALDI-TOF MS). Conclusion: The development of new technologies that allow rapid, sensitive, reproducible, and low-cost microbial identification, it is of great relevance for clinical microbiology, and consequently, for public health. Keywords: Bacteria. Genotyping techniques. Microbiological techniques ...


Research Article of American Journal of Histology and Cytology EVALUATION OF HEMOLYTIC ACTIVITY OF YANGAMBIN ISOLATED FROM Ocotea duckei VATTIMO-GIL Maria Isabel de Assis Lima Castro1*, Marta Gerusa Soares de Lucena1,2, Camilla Vila Nova Soares Silva3, Eliete Cavalcanti da Silva1,2 1Postgraduate Program in Morphotechnology - Federal University of Pernambuco, 2Department of Histology and Embryology - Federal University of Pernambuco, 3Multiprofessional Residency Program in Health Surveillance - Recife Health Department Yangambin, a lignan isolated from the leaves of Ocotea duckei Vattimo-Gil, has several pharmacological activities described in literature. However, few information about its toxicity has been reported. Red cells represent about 90% of blood cells and have interesting structural and molecular characteristics as experimental models in toxicological studies. Thus, the aim of this study was to evaluate the hemolytic action of yangambin in sheep blood. The hemolysis assay was performed at concentrations of 50, 25 and 12.5 µg/mL of yangambin in triplicate and hemolysis percentage was defined through the absorbance resulting from the test concentrations compared to the positive control. The results showed that yangambin did not cause hemolysis at the concentrations tested, therefore it did not cause damage to the plasma membrane of sheep erythrocytes. Keywords: Hemolytic activity, cytotoxicity, Yangambin ...


Research Article of American Journal of Histology and Cytology ANTIDEPRESSANT-LIKE EFFECT OF DIMETHYL FUMARATE AND ASTROCYTE CHANGES IN HIPPOCAMPUS OF MICE SUBMMITED TO MODEL OF DEPRESSION Alana Gomes de Souza1 *, Iardja Stéfane Lopes Sales1, Talita Matias Barbosa Cavalcante1, Michele Albuquerque Jales de Carvalho1, Leonardo Peixoto Fernandes1, Marta Maria de França Fonteles1 1UFC Introduction: Depression is a psychiatric condition of high prevalence worldwide and is associated with inflammation. Dimethyl fumarate, used to treat psoriasis and multiple sclerosis, has demonstrated an anti-inflammatory effect. Objective: To evaluate the effect of dimethyl fumarate on depression, through the immobility time in the forced swim test, and on astrocyte proliferation, through the expression of glial fibrillary acidic protein in the hippocampus of mice submitted to Chronic Unpredictable Mild Stress- induced model of depression. Methods: Male mice were divided into three groups: control(1), stressed(2), stressed+dimethyl fumarate (3). Groups 2 and 3 were subjected to 28 days of exposure to unpredictably applied stressors. From the 14th day, group 3 was treated with dimethyl fumarate (oral). At the end, the hippocampus was removed to determination of glial fibrillary acidic protein expression by immunofluorescence. Results: The immobility time was increased by chronic stress compared to the control and the treatment with dimethyl fumarate decreased this parameter, compared to the stressed group [F (9, 92) = 6,460; 1vs2: p <0.01, 2vs3: p <0.001]. Glial fibrillary acidic protein expression was reduced by chronic stress compared to control in the two areas of the hippocampus analyzed and treatment with dimethyl fumarate did not interfere in this parameter [CA1: F (2, 6) = 5.173, 1vs2: p <0.05; Dentate gyrus: F (2, 7) = 31.44, 1vs2: p <0.001]. Conclusion: Dimethyl fumarate has antidepressant-like effect in mice, however more studies need to be carried out to elucidate the neuroinflammatory mechanism of this drug. Keywords: chronic ...

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American Journal of Histology and Cytology1