American Journal of Histology and Cytology

Evaluation of the Factors Associated with the Success of In Vitro Fertilization Cycles among Infertile Couples in Qassim Region, Saudi Arabia

Research Article of American Journal of Histology and Cytology Evaluation of the Factors Associated with the Success of In Vitro Fertilization Cycles among Infertile Couples in Qassim Region, Saudi Arabia Agharid Abdul-Aziz ALsalahi and Amal Hussain Mohamed Ali Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Kingdom of Saudi Arabia The science gets developed and we can get fertilization process in vitro under special condition, (Wang, J, et al, 2006). Female age reflecting ovarian function, is predictor of pregnancy after in vitro fertilization (IVF). (L.L.van Loendersloot, et al, 2010), while Mediterranean diet may improve the IVF success rate among women. (Karayiannis D, et al., 2018). Thus, the aim of this study was to find out the factors that will associate with the success of IVF cycle among infertile couple in Qassim region. methods was a cross sectional study conducted at Prince Faisal Bin Mishaal fertility center in October,2019. (34) of fertility center patients willing to participate constituted the study sample. The data was collected using structured questionnaire to gather information on demographic characteristics, smoking habit, diet and supplement intake. data was analyzed in EPINFO v7 with descriptive and analytical statistics. results showing (94.12%) of successful IVF cycle. Most of females (92.9%) are under age of 40, whereas (66.7%) males were 40 or above. (9%) of total participants were smoker, (26%) had a partner who is a smoker. (65%) of the participants were neither them nor their partners a smokers. (38.24%) consume proteins (3-5) times a week, (55.88%) consume vegetable and fruits once a week, (35.29%) consume grains more than (5) times a week and (35.29%) consume dairy products (3-5) times a week. conclusion of study the pregnancy rate is higher in female who were under age of 40 but there is no significant association between age, smoking ...

Histological and ultrastructural studies of myocardium lesions produced by hair dye (para-phenylenediamine) in rats

Research Article of American Journal of Histology and Cytology Histological and ultrastructural studies of myocardium lesions produced by hair dye (para-phenylenediamine) in rats Aisha D. Alalwani Department of Biology, Faculty of Sciences, University of Jeddah, Saudi Arabia. Para-phenylenediamine (PPD) is an aromatic compound and usually applied in a few commercial and mechanical products. Additionally, ladies apply henna for coloring their hair, which contains PPD as one of the components. Henna is used in East Africa, India, and Middle Eastern countries as a part of their culture. However, it is rarely found in western countries. The aim of this study was to examine the effect of two different doses of PPD topical application on the skin of female rats. The microscopic analysis indicates that the body and heart weights were affected. Histopathological studies on cardiac fibers showed that the treatment of rats with 1.5 ml of PPD resulted in increased cytoplasmic vacuolization and the loss of myocardial cells with a globular nucleus. Additionally, an increased dose of 3 ml PPD showed several signs of cardiotoxic effects on increased cytoplasmic vacuolization and myofibrillar loss. Congestion, focal necrosis, swollen mitochondria, and lymphoid infiltration were also observed. Considering the findings of this study, it is concluded that the chronic usage of PPD promotes histopathological alterations in heart tissue of rats. Keywords: Para-phenylenediamine, rats, myocardium, cardiac fibers, histopathology ...

Leprosy in the Process of Development of Peripheral Neuropathy

Review Article of American Journal of Histology and Cytology Leprosy in the Process of Development of Peripheral Neuropathy Araújo, S.K.B.S¹; Dias, W.L.R²; Cavalcanti, A.V.F.S³; Silva, G.K.L.F4; Torres, R.L.N5; Mendonça, E.F6. 1,2,3,4,5Acadêmicos do Curso de Bacharelado em Enfermagem–ASCES-UNITA. 6Docente do Curso de Bacharelado em Enfermagem–ASCES-UNITA Introduction: Stigmatized since the biblical times, the leprosy is developed by Mycobacterium leprae, havinglike a of yours principal characteres the impairment of peripheral nerves that can to initiate in deficients and fisics deformities. Objectives: Analise the process of the peripheral neuropathy by leprosy. Methodology: It’s a revision of integrative role, whose reviewed articles were captured through database analysis: LILACS and SciELO. The descriptor sutilized were:Leprosy, Peripheral nerves, Quality of life and Treatment. The analysis were realized on october, 2017; the criterions of the included were published articles in portuguese and english, in 2012 to 2017, being excluded all that haven’t adequation at thematic of study. By means of the criterions defined were found 9 publisheds, but, only 2 articles met the criterions of the principal objective. Results and Discussions: The diagnostic of the patology is clinic starting by thermics stimuli, tactille and painful. In the start of the disease, the Scwann’s cells are the firsts commited, but, progressively the axonies also can to be affecteds. The neuropathy of the leprosy is of cronic course, presenting some acute phases. The principal nerves achieved are ulnar in the elbow, median in the handle and fingers, superficial radial in the handle, tibial in the tarsal tunnel, fibular, tunnel straight-fibular, beyond of the sensitives branches: saphenous, superficial peroni and sural. She cans to pass by evolutions and a present differentiated characteres, necessiting of accompaniment constant. The realization of neurologic exam like palpation of nerves and profund reflexions, sensitive mapping, motor voluntary test, dermatologic avaliations and anamnese, support in the early ...

In Silico Characterization of Nbs-lrr Gene Family in Vitis Vinifera Genome

Research Article of American Journal of Histology and Cytology In Silico Characterization of Nbs-lrr Gene Family in Vitis Vinifera Genome Silva, J.B.1, Silva, R.L.O2, Silva, I.L3, Silva, K.A4, Moura, M.T5, Benko-Iseppon, A.M6 ¹Mestranda do Programa de Pós-Graduação em Ciências Biológicas – UFPE, 2Pós-Doutoranda do Departamento de Ciências Biológicas da UFPE, 3Estudante do Curso de Bacharelado em Biomedicina – UNINASSAU Campus Campina Grande, 4Bacharel em Ciências Biológicas - UFPE; 5Pós-Doutorando do Departamento de MedicinaVeterinária da UFRPE, 6Docente/Pesquisadora do Departamento de Genética da UFPE Introduction: During evolution, plants developed defense mechanisms against pathogen attack, including an infinity of molecular processes that are triggered by pathogen exposure, such as resistance gene synthesis (R). The NBS-LRR gene family is known as one of the most representative families in the R gene class, in which its protein domains are related to one form of plant defense mechanisms. Objective: To identify and characterize candidate sequences of the NBS-LRR gene family in the Vitis vinifera genome. Methodology: Initially, a seed sequence was selected from those curated in the NBS-LRR family and deposited in the UniProt database. This sequence was aligned via tBLASTn against the V. vinifera genome deposited in the NCBI, by adopting a cut-off of e-value ≥ e-10. The sequences were annotated, translated and had its conserved protein domains identified by both ORF Finder and CD-search tools, respectively. Finally, the prediction of the isoelectric point was performed, and the molecular weight was also estimated using the JVirGel 2.0 software. Moreover, the subcellular localization was carried out by the Cell-PLoc 2.0 software. Results and Discussion: A total of 40 candidate sequences were retrieved that are related to the gene of interest. The translated proteins showed a variation from 338 to 944 aa in size. A total of 33 complete NBS domains were found, from which 16 sequences ...

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1.Santos, G. A.; Ramos, L. V. S.; Santana, J. F.; Lima, L. F. A; França, L. C.; Maia, C. S. Differential Diagnosis for Incisive Channel Cysts.American Journal of Histology and Cytology, 2018, 1:3. DOI: 10.28933/ajohc-2018-05-1803 
2.Soares, T. A. B; Cursino, G. T; Donato H. M. G; Luis J. M. S; Ferreira K. M. L. M; Souza M. B. R. Genetic Factors and Clinical Characteristics of Early Alzheimer. American Journal of Histology and Cytology, 2018, 1:2. DOI: 10.28933/ajohc-2018-05-1802 

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