International Congress 2018

Introduction: Foods of animal or vegetable origin, fresh or processed, including cheese, can carry several pathogenic microorganisms, thus causing disease to those who consume it. In particular, fresh hand-crafted cheeses because they are mostly made from raw milk and do not undergo ripening. Objective: To analyze the bacteriological quality of artisanal cheeses marketed in the free trade fairs of the city of Caruaru-PE. Methodology: The samples were collected in plastic bags and after collection, were transported in an isothermal container and sent to the laboratories of Food Technology and Food Microbiology of Centro Universitário Tabosa de Almeida. The research of total coliforms, thermotolerant and Pseudomonas aeruginosa was performed by the multiple tubes technique and the counting of heterotrophic bacteria was by the technique of pour plate both as recommended by the Standard Methods for the Examination of Water and Wastewater (APHA). Results and Discussion: Of the 50 rennet samples analyzed, all (100%) showed growth for the coliform group, and 49 samples (98%) showed growth for thermotolerant coliforms. Of these, 17 samples (34.69%) presented counts greater than or equal to 1600 NMP / g. Regarding the Pseudomonas count, 46 (92%) of the 6 fairs analyzed presented growth for the genus Pseudomonas sp. While 45 (90%) of the samples confirmed Pseudomonas aeruginosa, in addition, the heterotrophic bacteria count was above 105 CFU / g. As for the physical-chemical parameter (pH) of the analyzed samples, the results found ranged from 5.2 to 5.9. Conclusion: In view of the results, it was concluded that the high index of contamination by faeces (coliforms), organic matter (Pseudomonas aeruginosa) and contamination index (heterotrophic bacteria) may be related to the presence of pathogens above the values established by legislation valid. Thus becoming a product of poor conditions for human consumption, requiring a greater supervision.

Introduction: Diabetes Mellitus is a heterogeneous group of metabolic disorders characterized by chronic hyperglycemia and glucose intolerance, able to cause metabolic changes. Objective: To observe changes of female sex hormones in mice, after chemical induction of Diabetes Mellitus, in intervals of 15, 30 and 45 days. Methods: Adult females of the Swiss mice (35-40 g) had their weight and glucose levels evaluated. Animals were divided into three groups diabetics (D-15, D-30 and D-45) with their respective controls. Chemical induction of diabetes was held by alloxan monohydrate-solution (60 mg/mL), intraperitoneal, in concentration of 120g/kg. The control group was given saline solution. Ten days after induction, the animals underwent 8 hours of fasting and reassessed their weights and blood glucose levels. Were considered diabetic animals with blood glucose under 200 mg/dL. Animals which have not obtained this index were again submitted to the induction protocol. Blood glucose levels were measured at weekly intervals during the study period. The animals were sacrificed and blood collected immediately by cardiac puncture, packaged in gel tube and centrifuged to obtain serum. Hormone concentrations were determined by chemiluminescence method, in duplicate. The research was submitted to the Ethics Committee and approved (CEP 123-2013, UFCG-CTRS Campus Patos). The results that showed homogeneity and normality were evaluated by analysis of variance (ANOVA), with a significance level of 5% (p < 0.05). Results and discussion: The values for the Luteinizing Hormones (p = 0.000) and Estradiol (p = 0.029) showed significant only within 15 days of chemical induction. The Follicle Stimulating Hormone was significant in the periods of 15 and 45 days (p = 0.023 and p = 0.036, respectively) when compared to the control. Conclusion: The chemically induced diabetes probably set off hormonal changes, however, additional studies are required in a larger number of animals.

Introduction:Sexually transmitted infections (STIs) affect over 500 million people annually and female professionals (PSF) maintain a risk behavior for acquisition and transmission of STIs. Knowledge of STIs in this group is important in order to create a preventive public health policy program. Objective: To describe as major STIs that affect as PSF. Methodology: Bibliographic review of articles indexed in the international scientific database (PubMed) from September 2007 to September 2017, aged between 19 and 44 years. We selected 39 articles among which 24 were analyzed. Results and Discussion: A major STI for aid associated with Herpes Simplex Type 2 infection (83.2%), HPV (27%), vulvovaginitis (19.1%), Hepatitis B (10.7%), Syphilis (5.2%). These studies were from different countries dominating China. A prevalence of STIs is related to the vulnerability of FHPs, which, linked to the contexts of poverty, violence, alcohol and drug use, and non-legalization of the professionalization of sex in some places may contribute to marginalization of the risk situation and thus, . It was evidenced a low prevalence of syphilis in these studies, but not Brazil, an increase in STI since 2015 has been observed. The increase of STIs in the elderly population has been observed, due to an increase in the number of opportunities and availability of medications for erectile dysfunction, boosting life the elderly, making them more vulnerable to STIs. Conclusion: A public health alert is needed about the increased prevalence of PSFs caused by STIs. What has been happening due to the lack of use of condoms, multiple partners, but also due to lack of support for diagnostic accomplishment, treatment of recent reports that deepen or knowledge in this area, and may impel a development of preventive and assistance strategies aimed at improving sexual health and reproductive health of a marginalized and vulnerable population.

Introduction:Trichomonas vaginalis is the etiologic agent of trichomoniasis, a sexually transmitted infection associated with infertility, gestational complications, predisposition to cervical and prostate cancer, and increased risk of human immunodeficiency virus (HIV) transmission and infection. Metronidazole, the only drug available for treating, has dubious efficacy, high toxicity, it is contraindicated in first trimester of pregnancy and drug-resistant cases are increasing. Thus, there is an urgent need for the development of alternative strategies to combat trichomoniasis. In this context, lauric acid or dodecanoic acid, a fatty acid found in breast milk, coconut milk and coconut oil, has been used as an antimicrobial and antiparasitic compound. Aim: To evaluate the anti-proliferative effect of lauric acid on T. vaginalis. Methodology: Parasites were axenically cultured in TYM medium (Tryptone – Yeast Extract – Maltose) supplemented with bovine fetal serum and antibiotics for 28-36h at 37 ° C. Next, 105 parasites were transferred to eppendorf tubes, containing 500 μl of TYM medium with bovine fetal serum and antibiotics, and treated with 20, 50 and 100 μg/mL lauric acid at concentrations of (stock solution: 50 mg/mL) at 37 °C for 24 h. Parasites treated with DMSO and in the absence of lauric acid were used as controls. Cell growth was determined after 24h by direct counting in Neubauer chamber. Results and Discussion: Similar to described in other parasitic microorganisms, our data showed a growth inhibition of 23,27%, 39,57% and 53,53% when the parasites were treated with 20 μg/mL, 50 μg/mL and 100 μg/mL lauric acid, respectively. Conclusion: Lauric acid inhibited the proliferation of T. vaginalis in a dose-dependent manner after 24h of treatment. Further studies are needed to elucidate the action mechanisms of this compound on T. vaginalis.

Introduction:Climacteric processes and menopause are milestones in the women life, in which the first symptoms vary in each body. In order to reduce the discomfort caused by the climacteric and menopause, it is important to understand them. Objective: Disseminate information pertaining to identification of climacteric phenomena. Methodology: The case study occurred in a polyclinic from Cabedelo-PB in order to target academics to conduct humane and encouraging health education. Results and Discussion: M.S.A., 53 years search service in 16/08/2017 with chief complaint of dyspareunia, being routed by the urologist to evaluate possible low estrogenic for amenorrhea for three months and urinary incontinence. The anamnesis, reported irregularities in the menstrual cycle, flow reduction and shortening of the cycle (a: 13/08/17 for three days in a small amount and look like “coffee grounds”), and politeness. After the physical examination showed: flat and thin, vulva with rare sebaceous glands, dense, dry vaginal wall and urogenital atrophy, accompanied by fine as requested by ultrasound urologist. Reached the climacteric diagnostic hypothesis, FSH blood test, 2-estradiol, and prescribed estriol due peripheral action. It was subsequently opened a moment of dialogue with the patient about your knowledge of the subject and its aspirations, it became apparent that the lack of knowledge did identify themselves as “sick”, however, after the explanation of this physiological and mark the next stage of menopausal, we noticed a patient relief by decreasing your anxiety and helping in the understanding of the requested examinations and medication prescribed your membership and encouraging relationship doctor-patient. Conclusion: there was the urgent need to improve the knowledge about the menopause, to improve the attention and empower it, because it is a period of life characterized by physiological deficits which ovarian endocrine changes, provides psychological and somatic, known as climate syndrome, which has significant marker menopause.

Introduction: Cervico-vaginal cytology, or cervical cytology, has been the main method of detection and prevention of cervical cancer and studies the exfoliated cells of the squamocolumnar junction, as well as the detection of premalignant and malignant lesions, as well as the presence of pathogenic microorganisms. For the susceptibility to infections by carcinogenic viruses. Objective: The purpose of this work is to elucidate the frequency with which the microorganism is present in the cervico-vaginal cytology, providing an explanatory view of it. Methodology: This is a descriptive study of the type of bibliographic review on the subject, based on scientific articles published in the years 2000 to 2013 and journals indexed in the following databases SCIELO and Google academic, comparing the information from these sources comparatively of research. Results and Discussion: The vast majority of etiologic agents that can be found in the Pap smear. Among the microorganisms transmitted by sexual contact, such as Trichomonasvaginalis, which is also an infection frequently seen in pre-drugs, represents about 10 to 15% of the causes of vaginal discharge, Candidasp, corresponding to 58.54%; Gardnerellavaginalis (13.41%) Chlamydiasp, corresponding to 4.88%, Actinomycespp 16.1%, Mobiluncussp 2.0% Conclusion: As shown, it is inferred that a wide range of microorganisms can be identified in cervico-vaginal cytology, it is concluded that a significant portion of microorganisms can be analyzed through the Papanicolau method, being an important tool to detect agents of great clinical interest.

Introduction:Although the main task of the voice is communication, in modern society a third of the population depends on the voice as a primary instrument in their work. Among these professionals, the teacher can be highlighted as the voice professional with the highest occurrence of dysphonia. Objective: This article aims to analyze the perception of teachers about the vocal health workshop in a private school in the city of João Pessoa. Methodology: An exploratory research was conducted, with a sample of 7 teachers. A questionnaire was used as instrument, and a descriptive analysis was performed through SPSS software. The project was approved by the Ethics Committee of UNIPE with the approval number 1.829.531. Results and Discussion: The sample was 100% composed of women, ranging in age from 27 to over 50, with up to 20 years teaching. Among the participants, 57.1% said they had already lost their voice during work. Conclusion: It was concluded that through the vocal health workshop they were able to know the importance of care with the voice, but not all of them changed their habits towards the necessary care. It was also noticed that all of the participants presented symptoms for prolonged use of the voice, although they do not keep the exercises as part of their daily life. This signals for the importance of actions towards health promotion and prevention of these professionals.

Introduction: A deaf child may develop pedagogically at regular school levels, but facing difficulties by entering into the exclusive environment of listeners. In this context, facilitating methods can be used for secondary education and understanding of the deaf student. But an insight into teachers’ knowledge of methodologies is unknown. Objective: To explore how the teachers’ strategy for the teaching of reading and writing of the deaf. Methodology: It was a descriptive research with quantitative approach, carried out in a public school in the city of Joao Pessoa-PB. We interviewed ten (n = 10) teachers whose has in their student body some deaf student. The data were collected through the application of a structured questionnaire with six (n = 6) objective questions about teacher training and methods used in the classroom with deaf students. The data analysis was performed on the basis of Microsoft Word 13 program. Results and Discussion: It was observed that only 10% (n = 1) of teachers had academic education directed to the deaf student, most of the participants teachers have struggles with the relationship with the deaf student in the classroom, and despite the delay, more than 50% of them do not use any facilitative method. Conclusion: In light of the above, one can perceive a lack of knowledge of techniques and difficulties of relationship between teacher and deaf student. These only show off the importance of the presence of Educational Phonoaudiology, because it contributes to the awareness of the facilitating methods and collaborations for the better development of the student in the classroom.

Introduction: Aphasia is an acquired disorder of language processing due to brain damage, which can compromise expressive and receptive aspects. Music therapy can contribute both to the rehabilitation of the aphasic subjects’ linguistic abilities, facilitating verbal and non-verbal communication and strengthening neuropsychological functions, as well as modulation of emotions, improving mood and quality of life. Melodic Intonation Therapy (MIT) is a rehabilitation technique that proposes to develop verbal fluency and prosody by means of steps, in which sentences and prayers are used to produce the patient. Objective: Verify in the specialized literature the application and the efficacy of MIT with the support of music in patients with Expressive Aphasias, through selected articles. Methods: It was carried out an extensive research and articles published between 2011 and 2017, indexed in the data bases SCIELO, MEDLINE and LILACS, were selected. The following keywords were selected in portuguese: music, aphasia and expressive language. Results and Discussion: The MIT uses the supra-segmental aspects of speech, that is, of prosodic elements. In cases of expressive aphasia a function of the chant is preserved, because it activates the right cerebral hemisphere, which is the opposite side to an injury present in aphasic individuals. Thus, we verified that MIT adapted with the support of music, offer significant results in patients with aphasia of expression: improve in speech fluency, reduction of anomies, improvement in syntax of speech and dyspraxia of speech, and improvement of attention functions, memory of work, episodic-semantic verbal memory (recognition), prospective memory, naming, reading aloud, spontaneous writing and dictation. Conclusion: Aiming to restructure the language of patients with aphasic of expression, a MIT contributes not only to the rescue of communicative skills, but also to the social reinsertion of people and improving their quality of life.

Introduction:The hospital cinema consists of bringing patients, caregivers and health professionals to a new environment bringing about a modification of the reality they are facing. Cine Clube +, linked to the MAIS Program (Manifestation of Integrated Art to Health), aims to promote humanization thus improving the quality of life of those who attend. Objectives: use of the cinema as a complementary form in the treatment of diseases, health promotion, humanization and sensitization of the community of the Hospital das Clínicas of UFPE and gather areas of knowledge by approaching centers, departments and disciplines to jointly evaluate the benefits of cinema in spaces of health. Methodology: Are displayed weekly short films by students of the courses in the areas of health, human and exact of UFPE, responsible for: selection, organization, transportation of the material and development of sessions. sessions have duration of about an hour being directed to the whole community hospital and taken to several wards of HC-UFPE. Apply questionnaires pre and post intervention, in order to quantify results about the project and well being of spectators. Results and discussion: With a presence of 5 to 10 people on average at each session it is observed that the patients leave a troubled emotional state to a state of relaxation, joy, tranquility. Idleness gives way to laughter and distraction facilitating the release of hormones that will aid in the healing process. This relationship has achieved positive results, since it allows the withdrawal of patients from their routines and integrates the varied possibilities offered by the cinema, making them see and participate in a universe beyond the corridors of the hospital. Conclusion: The use of cinema serves as a facilitator of the treatment process, besides generating aggrandizement for the academic area by encouraging students to participate in humanized professionals.