In Vivo effects of dosage of leaf, bark and root extracts of V. paradoxa on diarhoea-induced albino rats

In Vivo effects of dosage of leaf, bark and root extracts of V. paradoxa on diarhoea-induced albino rats

M. B. FALANA 1*, M. O. BANKOLE2 and A. M. OMEMU3
1*Department of Biological Sciences, AL- Hikmah University Ilorin, P.M.B 1601, Nigeria. 2Department of Microbiology, Federal University of Agriculture, Abeokuta (FUNAAB), P.M.B 2240, Nigeria.3Department of Food Service and Tourism, Federal University of Agriculture, Abeokuta (FUNAAB), P.M.B 2240, Nigeria.

International Journal of Traditional and Complementary Medicine

In vivo study of different dosages (50mg/kg and 100mg/kg) of various solvent extracts (water, methanol, omidun and sterile omidun) of Leaf, Root and Bark of Vitellaria paradoxa were examined on male and female albino rats induced with diarrhoea- causing microorganisms (Enterohaemorrhagic Escherichia coli (EHEC ATCC 43889), Enteropathogenic Escherichia coli (EPEC ATCC 43887), Salmonella typhi, and Escherichia coli ATCC 25922). The rats were randomly assigned into four groups (A-D), group A rats infected with Enteropathogenic Escherichia coli (EPEC ATCC 43887) and later treated with ciprofloxacin, group B rats were infected with EPEC only, group C rats were infected with EPEC and treated with 50mg/kg concentration of methanol extract of leaf of V. paradoxa, while group D rats were not infected nor treated. The procedure was repeated for other test diarrhoea-causing microorganisms and 100mg/kg concentrations of all the solvent extracts of root and bark respectively. The rats were observed hourly for six hours for the presence or absence of diarrhoea. Haematological studies was conducted on the blood samples of the rats. All extracts of V. paradoxa have anti-diarrhoea effect at both concentrations tested against all the tested diarrhoeagenic bacteria. Although the type of diarrhoea-inducing microorganisms did not significantly affect the activity of the plant parts and 50mg/ml concentration of water extract exhibited more significant reduction in diarrhoea symptoms than 100mg/ml concentration of other solvent extracts. Bark and leaf extracts of V. paradoxa was also more effective at reducing symptoms of diarrhoea in the experimental rats than root extracts. Levels of the major blood parameters (White Blood Cell, Red Blood Cell, Haemoglobulin and Packed Cell Volume) were not significantly different (P>0.05) among the rats treated with the plant parts but slightly higher in rats treated with bark, followed by root and leaves.

Keywords: Omidun, Vitellaria paradoxa, Diarrhoeagenic

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How to cite this article:
M. B. FALANA, M. O. BANKOLE and A. M. OMEMU. In Vivo effects of dosage of leaf, bark and root extracts of V. paradoxa on diarhoea-induced albino rats.International Journal of Traditional and Complementary Medicine 2018, 3:8. DOI: 10.28933/ijtcm-2018-07-0401


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