American Journal of Histology and Cytology

Histological and ultrastructural studies of myocardium lesions produced by hair dye (para-phenylenediamine) in rats

Research Article of American Journal of Histology and Cytology Histological and ultrastructural studies of myocardium lesions produced by hair dye (para-phenylenediamine) in rats Aisha D. Alalwani Department of Biology, Faculty of Sciences, University of Jeddah, Saudi Arabia. Para-phenylenediamine (PPD) is an aromatic compound and usually applied in a few commercial and mechanical products. Additionally, ladies apply henna for coloring their hair, which contains PPD as one of the components. Henna is used in East Africa, India, and Middle Eastern countries as a part of their culture. However, it is rarely found in western countries. The aim of this study was to examine the effect of two different doses of PPD topical application on the skin of female rats. The microscopic analysis indicates that the body and heart weights were affected. Histopathological studies on cardiac fibers showed that the treatment of rats with 1.5 ml of PPD resulted in increased cytoplasmic vacuolization and the loss of myocardial cells with a globular nucleus. Additionally, an increased dose of 3 ml PPD showed several signs of cardiotoxic effects on increased cytoplasmic vacuolization and myofibrillar loss. Congestion, focal necrosis, swollen mitochondria, and lymphoid infiltration were also observed. Considering the findings of this study, it is concluded that the chronic usage of PPD promotes histopathological alterations in heart tissue of rats. Keywords: Para-phenylenediamine, rats, myocardium, cardiac fibers, histopathology ...

Leprosy in the Process of Development of Peripheral Neuropathy

Review Article of American Journal of Histology and Cytology Leprosy in the Process of Development of Peripheral Neuropathy Araújo, S.K.B.S¹; Dias, W.L.R²; Cavalcanti, A.V.F.S³; Silva, G.K.L.F4; Torres, R.L.N5; Mendonça, E.F6. 1,2,3,4,5Acadêmicos do Curso de Bacharelado em Enfermagem–ASCES-UNITA. 6Docente do Curso de Bacharelado em Enfermagem–ASCES-UNITA Introduction: Stigmatized since the biblical times, the leprosy is developed by Mycobacterium leprae, havinglike a of yours principal characteres the impairment of peripheral nerves that can to initiate in deficients and fisics deformities. Objectives: Analise the process of the peripheral neuropathy by leprosy. Methodology: It’s a revision of integrative role, whose reviewed articles were captured through database analysis: LILACS and SciELO. The descriptor sutilized were:Leprosy, Peripheral nerves, Quality of life and Treatment. The analysis were realized on october, 2017; the criterions of the included were published articles in portuguese and english, in 2012 to 2017, being excluded all that haven’t adequation at thematic of study. By means of the criterions defined were found 9 publisheds, but, only 2 articles met the criterions of the principal objective. Results and Discussions: The diagnostic of the patology is clinic starting by thermics stimuli, tactille and painful. In the start of the disease, the Scwann’s cells are the firsts commited, but, progressively the axonies also can to be affecteds. The neuropathy of the leprosy is of cronic course, presenting some acute phases. The principal nerves achieved are ulnar in the elbow, median in the handle and fingers, superficial radial in the handle, tibial in the tarsal tunnel, fibular, tunnel straight-fibular, beyond of the sensitives branches: saphenous, superficial peroni and sural. She cans to pass by evolutions and a present differentiated characteres, necessiting of accompaniment constant. The realization of neurologic exam like palpation of nerves and profund reflexions, sensitive mapping, motor voluntary test, dermatologic avaliations and anamnese, support in the early ...

In Silico Characterization of Nbs-lrr Gene Family in Vitis Vinifera Genome

Research Article of American Journal of Histology and Cytology In Silico Characterization of Nbs-lrr Gene Family in Vitis Vinifera Genome Silva, J.B.1, Silva, R.L.O2, Silva, I.L3, Silva, K.A4, Moura, M.T5, Benko-Iseppon, A.M6 ¹Mestranda do Programa de Pós-Graduação em Ciências Biológicas – UFPE, 2Pós-Doutoranda do Departamento de Ciências Biológicas da UFPE, 3Estudante do Curso de Bacharelado em Biomedicina – UNINASSAU Campus Campina Grande, 4Bacharel em Ciências Biológicas - UFPE; 5Pós-Doutorando do Departamento de MedicinaVeterinária da UFRPE, 6Docente/Pesquisadora do Departamento de Genética da UFPE Introduction: During evolution, plants developed defense mechanisms against pathogen attack, including an infinity of molecular processes that are triggered by pathogen exposure, such as resistance gene synthesis (R). The NBS-LRR gene family is known as one of the most representative families in the R gene class, in which its protein domains are related to one form of plant defense mechanisms. Objective: To identify and characterize candidate sequences of the NBS-LRR gene family in the Vitis vinifera genome. Methodology: Initially, a seed sequence was selected from those curated in the NBS-LRR family and deposited in the UniProt database. This sequence was aligned via tBLASTn against the V. vinifera genome deposited in the NCBI, by adopting a cut-off of e-value ≥ e-10. The sequences were annotated, translated and had its conserved protein domains identified by both ORF Finder and CD-search tools, respectively. Finally, the prediction of the isoelectric point was performed, and the molecular weight was also estimated using the JVirGel 2.0 software. Moreover, the subcellular localization was carried out by the Cell-PLoc 2.0 software. Results and Discussion: A total of 40 candidate sequences were retrieved that are related to the gene of interest. The translated proteins showed a variation from 338 to 944 aa in size. A total of 33 complete NBS domains were found, from which 16 sequences ...

Cell Death Pathways on Cancer Therapy

Review Article of American Journal of Histology and Cytology Cell Death Pathways on Cancer Therapy Neto, P. P. M 1; Silva, M. R. P 2; Soares, C. L. R 1; Lucena, C. C. O 3; Silvino, P 4; Silva, T. G 5 1Postgraduate Program of Biological Sciences, Federal University of Pernambuco - UFPE, 2Undergraduate student of Biological Sciences, Federal University of Pernambuco – UFPE; 3Postgraduate Program of Pharmaceutical Sciences, Federal University of Pernambuco - UFPE; 4 Postgraduate Program of Northeast Biotechnology Network - RENORBIO, Federal University of Pernambuco - UFPE; 5 Professor/Researcher on Department of Antibiotics, Federal University of Pernambuco - UFPE. Introduction: Cancer is a disease characterized by uncontrolled cell proliferation. The development of new drugs effective in the cancer treatment seeks the selectivity in inducing cell death. Different cell death pathways are known to date, the main ones are apoptosis, necrosis and autophagy. Objective: To describe the main cell death regulatory pathways and the possible targets of new anticancer drugs. Methodology: A bibliographic review of the scientific literature published in international journals was carried out. Results and Discussion: Apoptosis is a programmed cell death that controls the balance between death and tissue proliferation, and cancer cells are able to evade this mechanism. It can be induced by the extrinsic pathway, where receptors of the TNF- or CD95 family are activated on the cell surface, which subsequently activate cytosolic proteases, the caspases; or by the intrinsic pathway, where apoptosis is induced by the release of apoptogenic factors by mitochondria. Bcl-2 family proteins are highly involved in this pathway. Autophagy is a type of death in which the cell eliminates unhealthy cytoplasmic components by lysosomal degradation. The autophagic process is highly controlled, among others, by components of the PI3K-Akt-mTOR pathway, becoming key targets in anticancer therapy via autophagy induction. Finally, ...

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1.Santos, G. A.; Ramos, L. V. S.; Santana, J. F.; Lima, L. F. A; França, L. C.; Maia, C. S. Differential Diagnosis for Incisive Channel Cysts.American Journal of Histology and Cytology, 2018, 1:3. DOI: 10.28933/ajohc-2018-05-1803 
2.Soares, T. A. B; Cursino, G. T; Donato H. M. G; Luis J. M. S; Ferreira K. M. L. M; Souza M. B. R. Genetic Factors and Clinical Characteristics of Early Alzheimer. American Journal of Histology and Cytology, 2018, 1:2. DOI: 10.28933/ajohc-2018-05-1802 

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