Global Journal of Molecular Biology

Research Article of Global Journal of Molecular Biology Population Structure And Diversity Among Improved Cowpea Varieties From Senegal Based On Microsatellite Markers Awa Sarr1,2 , Amy Bodian1, Bassiaka Ouattara3, Mouhamadou Moussa Diangar4, Mbaye Ndoye Sall1, Elisabeth A.M.C. Diop1, Diaga Diouf2, Ndiaga Cissé1 1Centre d’Etude Régional pour l’Amélioration de l’Adaptation à la Sécheresse (CERAAS), Institut Sénégalais de Recherches Agricoles (ISRA), Thiès, Sénégal; 2 Laboratoire Campus de Biotechnologies Végétales, Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop (UCAD), Dakar Fann, Sénégal; 3 Université de Fada N’Gourma (U-FDG), Fada N’Gourma, Burkina Faso; 4 Institut Sénégalais de Recherches Agricoles (ISRA), ISRA CNRA de Bambey, ISRA/Center of Excellence of CERAAS, BP53, Diourbel, Senegal Cowpea (Vigna unguiculata) is used in West Africa for both human consumption and animal feeding. Despite its importance, the production of the crop is hampered by biotic and abiotic constraints. To overcome these constraints, the Senegalese Institute of Agricultural Research (ISRA) has set up a breeding program since 1960 leading to the release of productive varieties. Information of the genetic diversity for these varieties could improve the effectiveness of further breeding programs. The objectives of this study was to assess the genetic diversity and the population structure of 11 cowpea improved varieties from ISRA collection. Twelve (12) polymorphic microsatellite markers were used and the analysis of genotypic data showed a total of 39 alleles ranging from 2 (SSR6288, SSR6311 and SSR6827) to 6 (SSR6807) per locus. Polymorphism rate ranged from 8.33% (Mougne and Kelle) to 58.33% (Mouride) with an average of 31.06%. Intra varietal genetic diversity was very weak (0.012 to 0.091). The genetic similarity revealed that Melakh and Pakau varieties were genetically the closest while Bambey 21 and Sam were the most distant. These results are confirmed by the dendrogram, which also showed that Thieye, Leona, Kelle, Sam, Mougne and ...

Research Article of Global Journal of Molecular Biology The role of Zi2 during neural tube and neural crest development Gerald Muça* Department of Morfofunctional Modules, Faculty of Veterinary Medicine, Agricultural University of Tirana, Koder Kamez, 2021 Tirana-Albania The transcription factor Zic2 is member of Zic family, at early stages it has been involved in several processes during embryonic development and later on in morphogenesis and organogenesis. An important role has been attributed to Zic2 during the development of the neural system. It has been involved in neural tube and neural crest formation. Both process structures will form the central and peripheral neural system. Mutation of Zic2 provokes holoprosencephaly in humans and in mouse also spina bifida. To date, there is not well elaborated the specific mechanisms under which Zic2 affect neural tube formation and the differences may exist between mouse and human phenotype. Almost the same ambiguity is for the specific role of Zic2 during neural crest development. Here is given are resumed latest studies and are given new insight about the role of Zic2 in these two processes and its new target genes. Keywords: Zic2; neural tube; neural crest; cell proliferation; development ...

Research Article of Global Journal of Molecular Biology Hematological parameters and sorological detection of canine hemoparasitoses with SNAP 4DX Teixeira C.L1; Picanço K.R2; Silva F.J3; Alves A.J4; Souza Neto Júnior J.C5 1Zootechnist and Specialization in Clinical and Toxicology Analysis Student; 2Clinical and Toxicology Analysis Specialist; 3Specialization in Clinical and Toxicology Analysis Student - UFPE; 4Full Professor – UFPE; 5Doctor of Veterinary Medicine - UFRN Introduction: The SNAP 4Dx is a rapid test device that uses ELISA (Enzyme-Linked Immunosorbent Assay) technology manufactured by the IDEXX Laboratory (Maine, USA) for rapid diagnosis of hemoparasitosis in dogs. SNAP® ELISA technology utilizes unique conjugate and substrate that amplifies the results. The bidirectional flow process evidences the antigen-antibody binding, providing high sensitivity and reliable results. Objective: evaluation of hematological parameters and serological detection of hemoparasitoses in dogs with Snap 4Dx. Methodology: A cross - sectional, descriptive and observational clinical research was carried out between September 2014 and October 2014 at Pet Dream Veterinary Medical Clinic, located in the city of Recife / PE. The hemogram was performed using a manual technique using blood collected with EDTA. The detection of the antigen of Dirofilaria immitis and the antibodies of Anaplasma phagocytophilum, Anaplasma platys, Borrelia burgdorferi, Ehrlichia canis and Ehrlichia ewingii, was performed with SNAP 4DX Plus device. Results and Discussion: 62 dogs of different races were used, without specifying age or sex, attended at the Clinic. The blood samples were collected by venipuncture cephalic or jugular, according to the size of the animal. The samples were packed in eppendorf microtubes containing EDTA as anticoagulant. The differential count was performed through blood smearing, stained in panopticus, observed with a 100X immersion objective. The rapid test for serological diagnosis showed the following result: Ehrlichia canis (32.25%), Anaplasma platys (6.45%), Dirofilaria immitis (6.45%). Discussion: The results found in the ...

Research Article of Global Journal of Molecular Biology To evaluate the antimicrobial potential of Streptomyces spp. J181 against clinical isolates of S. pyogenes Melo T.S1; Oliveira C.V.J2; Amaral J.P.D3; Lima G.M.S4; Sena K.X.F.R4 1Student of the Pharmacy Course, UFPE; 2Student of the Postgraduate Program in Biological Sciences, UFPE; 3Student of the Biomedicine Course, UFPE; 4Teacher/Researcher of the Department of Antibiotics, UFPE. Introduction: Streptomyces comprise the genus of actinobacteria most studied, as they have the ability to synthesize a variety of bioactive metabolites, of these many are antibiotics. Streptococcus pyogenes is a gram-positive coccus belonging to Group A of Lancefield. Among streptococci, it is the most relevant clinical agent in infections of the oral cavity due cases of streptococcal pharyngotonsillitis and its sequels: rheumatic fever and glomerulonephritis. They are also related to other pathologies such as endocarditis, septic arthritis, cellulitis, pyoderma and scarlatina. Objective: To evaluate the antimicrobial potential of Streptomyces spp. J181 against clinical isolates of S. pyogenes. Methodology: Blocks of 10 mm diameter obtained from the cultivation of the actinobacteria seeded on ISP-2 agar were transferred to Petri dishes containing 18 mL of blood agar previously seeded with S. pyogenes, then the plates were incubated at 35 ° C ± 1 ° C for 24 hours. After the incubation period, the inhibition halos were read in millimeters. To assess the susceptibility profile, the antibiogram of the bacterial isolates was carried out according to the recommendations of the Clinical & Laboratory Standards Institute. The tests were performed in triplicate, the results expressed in average plus standard deviation and the coefficient of variation adopted was ≤ 10%. Results and Discussion: The susceptibility profile of S. pyogenes isolates didn’t show resistance to the evaluated antibiotics, resulting in sensitivity to therapy commonly adopted in the medical clinic. The lineage of Streptomyces spp. J181 ...