Global Journal of Molecular Biology


Research Article of Global Journal of Molecular Biology DIVERSITY AND POPULATION STRUCTURE OF LOCAL AND EXOTIC LABLAB PURPUREUS ACCESSIONS IN KENYA AS REVEALED BY MICROSATELLITE MARKERS Eliezah M. Kamau1*, Miriam G. Kinyua2, Charles N. Waturu1, Oliver Kiplagat2, Bramwel W. Wanjala1, Robert K. Kariba3 and David R. Karanja1 1Kenya Agriculture and Livestock Research Organization. P.O. Box 57811-00200, Nairobi, Kenya. 2University of Eldoret, School of Agriculture and Biotechnology. P.O. BOX 1125, Eldoret, Kenya. 3World Agroforestry P.O. BOX 30677-00100, Nairobi Lablab purpureus is an important pulse crop in some parts of sub-Saharan Africa and Asia but has largely remained underutilized. Understanding the genetic diversity is prerequisite for genetic improvement and utilization of this leguminous crop. The relationships of the local lablab genotypes and those collected from other diverse geographic origins including the wild accessions remain unknown in Kenya. The study was undertaken to determine genetic diversity and population structure of germplasm accessions collected from Kenya and other global regions. Eight simple sequence repeat primer pairs were used to genotype the 189 lablab accessions. A total of 39 alleles were revealed by eight SSR with an average of 4.88 alleles per polymorphic loci. The average PIC was 0.42. The gene diversity among the accessions ranged from 0.26 to 0.52 with an average of 0.38, indicating moderate genetic diversity. Germplasm collected from Kenya showed a moderate genetic diversity of 0.36. Higher genetic diversity (He<0.5) was detected within the Ethiopian and South Africa populations. Analysis of molecular variance (AMOVA) revealed that 94% of the allele diversity was attributed to individuals within populations while only 6% was distributed among the populations. The Bayesian model-based Structure method and Principal coordinate analysis (PCoA) scatter plot clustered the accessions into three groups  with germplasms collected from Kenya showing distribution among all the three groups. The wild accessions clustered mainly with ...

Population Structure And Diversity Among Improved Cowpea Varieties From Senegal Based On Microsatellite Markers

Research Article of Global Journal of Molecular Biology Population Structure And Diversity Among Improved Cowpea Varieties From Senegal Based On Microsatellite Markers Awa Sarr1,2 , Amy Bodian1, Bassiaka Ouattara3, Mouhamadou Moussa Diangar4, Mbaye Ndoye Sall1, Elisabeth A.M.C. Diop1, Diaga Diouf2, Ndiaga Cissé1 1Centre d’Etude Régional pour l’Amélioration de l’Adaptation à la Sécheresse (CERAAS), Institut Sénégalais de Recherches Agricoles (ISRA), Thiès, Sénégal; 2 Laboratoire Campus de Biotechnologies Végétales, Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Cheikh Anta Diop (UCAD), Dakar Fann, Sénégal; 3 Université de Fada N’Gourma (U-FDG), Fada N’Gourma, Burkina Faso; 4 Institut Sénégalais de Recherches Agricoles (ISRA), ISRA CNRA de Bambey, ISRA/Center of Excellence of CERAAS, BP53, Diourbel, Senegal Cowpea (Vigna unguiculata) is used in West Africa for both human consumption and animal feeding. Despite its importance, the production of the crop is hampered by biotic and abiotic constraints. To overcome these constraints, the Senegalese Institute of Agricultural Research (ISRA) has set up a breeding program since 1960 leading to the release of productive varieties. Information of the genetic diversity for these varieties could improve the effectiveness of further breeding programs. The objectives of this study was to assess the genetic diversity and the population structure of 11 cowpea improved varieties from ISRA collection. Twelve (12) polymorphic microsatellite markers were used and the analysis of genotypic data showed a total of 39 alleles ranging from 2 (SSR6288, SSR6311 and SSR6827) to 6 (SSR6807) per locus. Polymorphism rate ranged from 8.33% (Mougne and Kelle) to 58.33% (Mouride) with an average of 31.06%. Intra varietal genetic diversity was very weak (0.012 to 0.091). The genetic similarity revealed that Melakh and Pakau varieties were genetically the closest while Bambey 21 and Sam were the most distant. These results are confirmed by the dendrogram, which also showed that Thieye, Leona, Kelle, Sam, Mougne and ...

The role of Zi2 during neural tube and neural crest development

Research Article of Global Journal of Molecular Biology The role of Zi2 during neural tube and neural crest development Gerald Muça* Department of Morfofunctional Modules, Faculty of Veterinary Medicine, Agricultural University of Tirana, Koder Kamez, 2021 Tirana-Albania The transcription factor Zic2 is member of Zic family, at early stages it has been involved in several processes during embryonic development and later on in morphogenesis and organogenesis. An important role has been attributed to Zic2 during the development of the neural system. It has been involved in neural tube and neural crest formation. Both process structures will form the central and peripheral neural system. Mutation of Zic2 provokes holoprosencephaly in humans and in mouse also spina bifida. To date, there is not well elaborated the specific mechanisms under which Zic2 affect neural tube formation and the differences may exist between mouse and human phenotype. Almost the same ambiguity is for the specific role of Zic2 during neural crest development. Here is given are resumed latest studies and are given new insight about the role of Zic2 in these two processes and its new target genes. Keywords: Zic2; neural tube; neural crest; cell proliferation; development ...

Hematological parameters and sorological detection of canine hemoparasitoses with SNAP 4DX

Research Article of Global Journal of Molecular Biology Hematological parameters and sorological detection of canine hemoparasitoses with SNAP 4DX Teixeira C.L1; Picanço K.R2; Silva F.J3; Alves A.J4; Souza Neto Júnior J.C5 1Zootechnist and Specialization in Clinical and Toxicology Analysis Student; 2Clinical and Toxicology Analysis Specialist; 3Specialization in Clinical and Toxicology Analysis Student - UFPE; 4Full Professor – UFPE; 5Doctor of Veterinary Medicine - UFRN Introduction: The SNAP 4Dx is a rapid test device that uses ELISA (Enzyme-Linked Immunosorbent Assay) technology manufactured by the IDEXX Laboratory (Maine, USA) for rapid diagnosis of hemoparasitosis in dogs. SNAP® ELISA technology utilizes unique conjugate and substrate that amplifies the results. The bidirectional flow process evidences the antigen-antibody binding, providing high sensitivity and reliable results. Objective: evaluation of hematological parameters and serological detection of hemoparasitoses in dogs with Snap 4Dx. Methodology: A cross - sectional, descriptive and observational clinical research was carried out between September 2014 and October 2014 at Pet Dream Veterinary Medical Clinic, located in the city of Recife / PE. The hemogram was performed using a manual technique using blood collected with EDTA. The detection of the antigen of Dirofilaria immitis and the antibodies of Anaplasma phagocytophilum, Anaplasma platys, Borrelia burgdorferi, Ehrlichia canis and Ehrlichia ewingii, was performed with SNAP 4DX Plus device. Results and Discussion: 62 dogs of different races were used, without specifying age or sex, attended at the Clinic. The blood samples were collected by venipuncture cephalic or jugular, according to the size of the animal. The samples were packed in eppendorf microtubes containing EDTA as anticoagulant. The differential count was performed through blood smearing, stained in panopticus, observed with a 100X immersion objective. The rapid test for serological diagnosis showed the following result: Ehrlichia canis (32.25%), Anaplasma platys (6.45%), Dirofilaria immitis (6.45%). Discussion: The results found in the ...

Dr. Hala Abdel Wahab
Ph.D., Université de Paris-Sud, France, Associate Professor, Department of Plant Pathology Head of Lab. “Molecular Diagnostic of Plant Diseases” Faculty of Agriculture, Ain Shams University, Cairo, Egypt

Dr. Shahin Gavanji
Chairman of “WAMS National Council of Iran at World Academy of Medical Sciences (WAMS), Chairman WAMS Oncology Research Group of Iran, 3-Young Researchers and Elite Club, Khorasgan Branch, Islamic Azad University, Khorasgan, Isfahan, Iran, Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan

Dr. Richa Madan Lomash
Research Fellow, Receptor Biology Unit (NINDS), NIH, Bethesda (MD), USA

Dr. Alessandra Pelagalli
School of Veterinary Medicine, University of Naples

Professor, Department Of Chemistry, Sri Vidya Nikethan Engineering College.Tirupathi ,INDIA

Dr Jahwarhar Izuan Bin Abdul Rashid
Department of Chemistry & Biology, Centre for Defence Foundation Studies (CDFS), Defence National University of Malaysia

Dr. Omi Laila Majeed
Division of Basic Sciences and Humanities, Sher-e-Kashmir University of Agricultural Sciences and Technology

Dr. Mohammad Mehdizadeh
PhD of Weed Science, University of Mohaghegh Ardabili

Dr Amanjot Kaur Riar
Brigham and Women’s Hospital- Harvard Medical School, Department of Medicine, Harvard New Research Building USA

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1.Teixeira C.L; Picanço K.R; Silva F.J; Alves A.J; Souza Neto Júnior J.C.Hematological parameters and sorological detection of canine hemoparasitoses with SNAP 4DX.Global Journal of Molecular Biology, 2018, 1:5. DOI: 10.28933/gjmb-2018-05-1805 
2.Melo T.S; Oliveira C.V.J; Amaral J.P.D; Lima G.M.S; Sena K.X.F.R. To evaluate the antimicrobial potential of Streptomyces spp. J181 against clinical isolates of S. pyogenes.Global Journal of Molecular Biology, 2018, 1:4. DOI: 10.28933/gjmb-2018-05-1804 

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Global Journal of Molecular Biology