Bio-enhancement effect of Bos primigenius indicus urine isolates on Curcumin anticancer activity using different human cell line models of A549, Hep-G2, MCF-7, Jurkat and K562
The study is conducted to explore possible utilization of cow urine and understanding complete biochemical compounds involved in possible therapeutic applications of cow urine therapy which was used from ancient times. In the present experiment inductively coupled plasma mass spectrometry (ICP-MS) and Gas Chromatography-Mass Spectrometry (GC-MS) methods was used to analyze the complete mineral and compound detection. The test conducted using MTT method using human cancer A549, Hep-G2, MCF-7, Jurkat and K562 cell lines as part of the in vitro preclinical characterization of Cow urine extracted Curcumin and pure Curcumin compounds and compared against positive control Sodium lauryl Sulphate (SLS). More than 100% increment in cell killing at a concentration of 1.25 mg/ml recorded in the cell line for the cow urine extracted Curcumin and less than 70% for the pure Curcumin. Positive control SLS exhibited nearly 100% of killing cells.
Antibacterial, antifungal and wound healing potentials of extract and fractions of Nauclea diderrichii root bark
gar dilution technique to determine the inhibition zone diameters (IZD) and minimum inhibitory concentrations (MICs). The excision wound model was used to assess the wound healing properties of MDE (5, 10 and 20%) and compared it with that of bacitracin and neomycin powder (BNP, standard) in rats. Phytochemical screening and acute toxicity test were performed using standard procedures. Results showed that extract and fractions exhibited antimicrobial effect with IZD (mm, 40 mg/ml) against tested organisms. The EF exhibited the best antimicrobial activity with IZD of 30, 8, 15, 8 and 12 against Staphylococcus aureus, Staphylococcus cohnii, Pseudomonas aeruginosa, Klebsiella aerogenes and Aspergillus flavus, respectively. The EF also gave an MIC (mg/ml) of 0.63, 20.00, 1.25, 20.00 and 0.63 for S. aureus, S. cohnii, P. aeruginosa, K. aerogenes, and A. flavus, respectively. The MDE showed a comparable wound healing effect with BNP, although with better effect on day 20 with 100% wound contraction while BNP gave 93.79%, post wound excision. Therefore, N. diderrichii root bark possesses antibacterial, antifungal and wound healing properties.
Ethnomedicinal uses of indigenous plant species in Mogalakwena Municipality of Waterberg District, Limpopo Province South Africa
Various indigenous cultures in the Limpopo Province of South Africa are transitioning from a traditional lifestyle to a westernised one. This leads to a loss of cultural identity and knowledge. Consequently, an ethnobotanical survey was conducted to investigate the medicinal uses of indigenous plants in the Mogalakwena Local Municipality of the Waterberg District in Limpopo Province, South Africa, an area experiencing rapid transition to a western lifestyle. The study interviewed 30 traditional healers via a semi-structured questionnaire. Thirty five indigenous plant species were documented to be used in the treatment of 37 ailments. These species are distributed among 22 families, of which Asteraceae (5 species), Fabaceae (4 species) and Hyacinthaceae (3 species) are the most prominent. The remaining families are represented by either a single or two species. Bark and roots accounted for 50% of the material used, followed by leaves (12%) and tuber (8%), amongst other. Preparation of remedies was mainly through decoctions (40%), followed by infusions (33.3%), paste (8.3%), and fumes through burning (6.7%). The high percentage of bark and roots use is an area of concern. Furthermore this study found many instances of unsustainable harvesting techniques. These issues need immediate attention for the long term conservation of a number of threatened species identified in this study.
The State of Chhattisgarh has about 44% of its geographical Area Covered with forests. The Koria district in Chhattisgarh lies between 22058’ to 23049’ North latitudes and 810 33’ to 82045’ East longitude. The average rainfall is 121.36 cm. The forest area is 81.23% of Total dist. area. The annual mean temperature is 240C. The temperature varies between 16.20C to 310C. Geologically the area is dominated by upper Gondwana rocks. Which are rich in coal deposit. The highest mountain ranges of the region occupy the northern part of the district. The district Koria has a very rich flora exhibiting diversity specially of medicinal plants. There is no comprehensive description of the flora of the district is available . The district has a tribal population using enormous range of plants for their basic needs, sustenance and livelihood.“Keeping these points in view” the Present paper deals with diversity of the medicinal plants of the district and their ecological status. Vegetational analysis of Bharatpur block revealed some interesting observations on phytosociological characters enumerate medicinal plants belonging to Bharatpur block enumerate 80 medicinal plants were recorded. the common plant species showing maximum frequency were Tribulus terrestris (90%), Vicia sativa (80%) and Jatropha curcas, Cleome gynandra and Blumea lacera (70%). At this block, Woodfordia fruticosa, Xanthium strumarium and Pergularia extensa were showing maximum frequency of 80%, 70% and 60% respectively. Woodfordia fruticosa showed high density also. Triumfetta rhomboidea showed maximum density and abundance but Bacopa monnieri and Vanda roxburghii showed maximum abundance.It was abstracted that Bacopa monnieri, Costus speciosus, Curculigo orchioides, Curcuma amada and Embelia robusta were the rare species. They exhibited only 20% frequency. Leea macrophylla was also very rare having only 10% frequency and only density of 1. Curculigo orchioides and Pterocarpus marsupium were among the less abundant species at this site.
Ethnomedicinal uses of exotic plant species in Mogalakwena Municipality of Waterberg District, Limpopo Province South Africa
Traditional medicine still constitutes a large part of the primary health care sector in South Africa. However, limited information exists about the ethnomedicinal uses of exotic plant species. Thus an ethnobotanical survey was conducted to investigate its use in Mogalakwena Local Municipality of Waterberg District of the Limpopo Province, South Africa. This was done by interviewing 30 traditional health practitioners, from 15 villages between April and November 2015. The study documented 8 exotic plant species that are used to treat various diseases. The documented species are distributed across 7 families, of which the Asteraceae (2) is the most prominent, while the remaining families are represented by single species. The plant parts, most used was roots (36.4%), followed by fruits (27.3%), whole plant (18.1%), whereas leaves and flower contributed 9.1% each. It was further evidenced that preparation of remedies were mainly through infusions (30%) and decoctions (30%), administered orally. It is concluded that exotic plants now form an integral part of the materia medica of Bapedi traditional health practitioners. It is further concluded that with proper and careful management exotic plant species can reduce overexploitation of heavily harvested indigenous medicinal plants in South Africa.
Diabetes and arterial hypertension resorts of treatments and plants used for their treatments in three phytogeographic areas of Cameroon
There are three principal resorts of diseases’ treatment in Africa that include the mixed traditional and conventional medicine, the pure traditional medicine and the pure conventional medicine. The objective of this study was to determine the importance of resorts of treatment for selecting efficacious medicinal plants used for the management of diabetes and/or arterial hypertension in Cameroon. The resorts of diabetes and/or arterial hypertension treatment preferred by 1131 Cameroonians, selected in 58 socio-cultural groups and plants used were evaluated. This sample of interviewees was distributed as follow: 293 in coastal dense humid rain forests (phytogeographic area 1), 577 in continental dense humid rain forests (phytogeographic area 2) and 561 in Guinean and Soudano-Zambesian savannahs (Phytogeographic area 3). The combination of traditional and conventional medicine was the most favorable therapeutic resort of diabetes and/or arterial hypertension treatment (58,89%), following by pure traditional medicine (38,72 %) and pure conventional medicine (2,39%). These main resorts present manifold variances in many social groups including. The more important variances include Self herbal medication and consultation of traditional healers (21,75%); self herbal medication and consultation of medical doctors (33,08%); traditional healers, self herbal medication and consultation of medical doctors (22,64%). The percentages in brackets correspond to the proportion of interviewees recorded. The interviewees who preferred traditional medicine know more available and efficacious medicinal plants. Therefore with their aid 33 plants which relieve the health condition of some diabetic and/or hypertensive suspected or indirect and confirmed patients were recorded. The most important of these plants that include Azadirachta indica, Momordica charantia, Phyllanthus amarus, Phyllanthus niruri, Laportea ovalifolia, Ceiba pentandra, Allium cepa, Persea americana and Catharanthus roseus relieve the followed up diabetic with hypertension patients. These plants revealed interesting for the potential management of diabetes and hypertension. Meanwhile the recorded plants needed to be detailed investigated for their…
The present investigation has been studied with the green synthesis of silver nanoparticles (AgNPs) using medicinally valued Adhatoda vasica -Nees and to evaluate the antibacterial and anticancer activity against HEP-G2 (Human epithelium cells of liver cancer) cell lines. The UV-Vis spectroscopy results show a strong resonance centered on the surface of silver nanoparticles at 420 nm. Fourier transform infrared (FT-IR) spectroscopy study demonstrates A. vasica aqueous extract acted as the reducing and stabilizing agent during the synthesis. The X-ray diffraction (XRD) analysis confirmed that the synthesized AgNPs are single crystalline face-centered cubic in structure, average crystal size 21 nm. Scanning electron microscope–energy dispersive spectroscopy (SEM-EDS) image confirmed synthesis of relatively uniform nanoparticles. The EDS analysis of the nanoparticles dispersion, using a range of 2-4 keV, confirmed the presence of elemental silver, without any contamination. The antibacterial activities were carried out against pathogenic bacteria. The maximum zone of inhibition was observed in the synthesized AgNPs (10µg/mL) against Staphylococcus sp. (16mm), Klebsiella sp. (14.5mm). The cytotoxicity activity as evidence by MTT assay with HEP-G2 cell lines. The synthesized AgNPs are ready for the application in the field of nanomedicine against pathogenic bacteria and very good anticancer drug.
As per WHO, in South-east Asia region alone 96 million people are estimated to be suffering from diabetes. Due to sedentary lifestyle and stress full nature of work, associated risk factors such as being overweight or obese, the number of diabetic patients is increasing. Diabetes is an important cause of blindness, kidney failure, lower limb amputation and other long-term consequences that impact on quality of life significantly. Continuous research is being done by scientists of various fields in order to achieve an effective cure of the disease and to improve quality of life. A great deal of work has also been done by Ayurvedic research scholars on various herbal and mineral drugs to find an effective treatment for diabetes generally reffered to as Prameha. Shilajatu is one such drug which has been described for the management of Prameha in Ayurveda texts. The present study was conducted to evaluate the efficacy of Shilajatu in the improving the quality of life in patients of Prameha. Twenty two diabetic patients were selected for the study. The results were assessed in terms of symptomatic relief on the basis of scoring system, as per the Questionnaire on Quality of Life. Statistically highly significant improvement (p
Bhaishajya kalpana is a very well developed sub-discipline of Ayurveda entirely devoted to drug formulations. Sharangadhara Samhitha is an epitome of Ayurvedic literature of medieval India. Since the drug manufacturing part is elaborately dealt in this, it is considered as an authentic text book of Bhaishajya Kalpana. It is one pioneering work, aptly included in the list of Laghutrayi, the other two being Bhavaprakasha & Madhava nidana. Acharya Sharngadhara, son of Damodara is considered as the author of this text. Except giving out his name as the author at the commencement of the text, he has not furnished any information about himself or his other works. Sharngadhara Samhita composed of 3 parts or khandas in which 32 chapters and 2600 verses are explained. This book is designed in a simple and easy language to serve as a handbook and thus it shines in the pure hearts of Scholars and Practitioners.
Bharat ,now known as India, by the world, is full of knowledge with advances since thousands of years. The nation has its own culture and healthy socio-economic life style since ancient time..This review article is concerned with its medical facilities that were available since ancient time in this continent. Ayurved is special boon to all the world by Bharat.Ayurved is not only the system of medicine but is a science of life…a direction to live healthy and long life.